Induction of mud crab (<i>Scylla paramamosain</i>) tropomyosin and arginine kinase specific hypersensitivity in BALB/c mice

Li, Bing; Yu, Huilin; Cao, Min‐Jie; Cai, Qiu-Feng; Lin, Jiang-Wei; Su, Wen‐Jin

doi:10.1002/jsfa.4565

Cited by 8 publications

(11 citation statements)

References 32 publications

(40 reference statements)

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“…This was the same phenomenon that appeared in our experiment. Furthermore, there were no typical allergic symptoms in mice according to the evaluation of allergic symptoms [6]. Thus, crayfish GIG may possibly contain one or more hazardous substances that can cause rhabdomyolysis in some mice.…”

Section: Discussionmentioning

confidence: 94%

“…The test results of the experimental groups were determined to be positive if the numerical value of sera IgE exceeded 2.1 times the values obtained in the control groups [6]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The animals were fed under controllable temperature (22 ± 2 °C), humidity (50−70 %), and lighting (12 h-light and 12 h-dark cycle, lights were turned on at 7:00 a. m.). Food and water were provided ad libitum [6].…”

Section: Animalsmentioning

confidence: 99%

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Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

et al. 2015

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

et al. 2015

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“…There are 3 previous elegant studies that reported on the development of shrimp allergy and 1 on mud crab allergy using mouse models with BALB/c and C3H/HeJ mice [25,26,27,28]. Thus, Capobianco et al [27] reported a shrimp tropomyosin allergy mouse model using C3H/HeJ mice with oral exposure to purified shrimp tropomyosin along with cholera toxin adjuvant; their readouts of allergenicity included sIgE (OD values; no titers), spleen-cell IL-4 production and systemic anaphylactic symptom scores upon OC.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are relatively few efforts reported in the literature on the development of animal models. Three reported mouse models of shrimp tropomyosin allergy and 1 of mud crab allergy share the following features: (1) the use of adjuvants (cholera toxin/complete Freund's adjuvant) and (2) the use of either systemic (intraperitoneal injections) or oral exposure to induce sensitization [25,26,27,28]. These models are highly valuable and useful for studying SF allergy mechanisms in the context of coexposure of allergens with powerful adjuvants.…”

Section: Introductionmentioning

confidence: 99%

An Adjuvant-Free Mouse Model of Transdermal Sensitization and Oral Elicitation of Anaphylaxis to Shellfish

Parvataneni

Gonipeta

Acharya

et al. 2015

Int Arch Allergy Immunol

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Background: Shellfish (SF) allergy is a leading cause of systemic anaphylaxis in humans. An adjuvant-free mouse model to evaluate allergenicity and oral anaphylaxis to SF is currently unavailable. Here, we tested the hypothesis that transdermal exposure (TDE) to SF protein extract (SFPE) not only elicits a systemic allergic immune response but also will clinically sensitize mice for oral anaphylaxis. Methods: Adult BALB/c female mice (6-8 weeks of age) were exposed to saline or SFPE once a week for 4 weeks using a transdermal sensitization method. Systemic SF-specific IgE, IgG1 and IgG2a and total (t)IgE responses were measured using ELISA. Systemic anaphylaxis upon oral SFPE administration was assessed according to clinical symptoms and the hypothermia shock response (HSR). Using individual mouse data, the correlation between the readouts of allergenicity was determined using Pearson's analysis. Spleen-cell IL-4 and IFN-γ responses were determined using primary cell culture and ELISA. Results: TDE to SFPE resulted in marked systemic specific (s)IgE, tIgE, IgG1 and IgG2a responses. Oral challenge with SFPE in sensitized mice (but not controls) elicited systemic anaphylactic clinical reactions and HSR. A strong correlation was observed between sIgE, tIgE and HSR. Spleen cells isolated from allergic mice (but not controls) exhibited memory IL-4 and IFN-γ cytokine responses. Conclusion: We report a novel adjuvant-free mouse model of SF allergy with robust quantifiable and correlated readouts of allergenicity that may be used in basic biomedical, preclinical and applied food/nutrition research on SF allergy.

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Current Immunological and Molecular Biological Perspectives on Seafood Allergy: A Comprehensive Review

Leung

Wai

Shu

et al. 2012

Clinic Rev Allerg Immunol

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Seafood is an important component in human diet and nutrition worldwide. However, seafood also constitutes one of the most important groups of foods in the induction of immediate (type I) food hypersensitivity, which significantly impacts the quality of life and healthcare cost. Extensive efforts within the past two decades have revealed the molecular identities and immunological properties of the major fish and shellfish allergens. The major allergen involved in allergy and cross-reactivity among different fish species was identified as parvalbumin while that responsible for shellfish (crustaceans and mollusks) allergy was identified as tropomyosin. The cloning and expression of the recombinant forms of these seafood allergens facilitate the investigation on the detailed mechanisms leading to seafood allergies, mapping of IgE-binding epitopes, and assessment of their allergenicity and stability. Future research focusing on the immunological cross-reactivity and discovery of novel allergens will greatly facilitate the management of seafood allergies and the design of effective and life-long allergen-specific immunotherapies.

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Induction of mud crab (Scylla paramamosain) tropomyosin and arginine kinase specific hypersensitivity in BALB/c mice

Abstract: These results indicated that a BALB/c mouse model for sensitization to TM and AK from mud crab was successfully established, and the Th2 response was observed, displaying increased immunoglobulin E levels, together with the production of interleukin 4 and allergic symptoms.

Cited by 8 publications

References 32 publications

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

An Adjuvant-Free Mouse Model of Transdermal Sensitization and Oral Elicitation of Anaphylaxis to Shellfish

Current Immunological and Molecular Biological Perspectives on Seafood Allergy: A Comprehensive Review

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