Induction of micronuclei and nuclear abnormalities by cyclophosphamide and colchicine inXenotoca melanosoma(Pisces, Goodeidae) from Lake La Alberca in Michoacán, México

Abstract

This study is a follow-up of previous research in which we described the frequency of spontaneous micronucleated erythrocytes (MNE) in the Goodeid Xenotocoa melanosoma collected from Lake La Alberca, located in the state of Michoacan, Mexico. In the present work, we measured micronuclei (MN) and nuclear abnormalities (NA) in erythrocytes of peripheral blood. Bioassays taken at 24 or 96 hours in either the cyclophosfamide (CP) or colchicine (COL) showed a significant increase in MN and BC (P values ranging from 0.0499 to 0.0036) compared with information from wild organisms collected over 3 years. Concentrationdependent and time-dependent responses support the proposal of using endemic Xenotoca melanosoma as a bioindicator of genotoxicity and cytotoxicity with a high transcendence for the health of the entire ecosystem and evaluation of the Lerma-Chapala watershed.

Potential Uses, Limitations, and Basic Procedures of Micronuclei and Nuclear Abnormalities in Buccal Cells

“…Indeed, MN presence has been used as a biomarker of genotoxicity in animals [27–31] and vegetables [32]. …”

“…The presence of MN can be evaluated in many tissues involving any dividing cell [ 25 ], for example, cervix epithelia [ 26 ], bladder, esophagus, and bronchial, nasal, and buccal mucosa [ 3 , 4 ]. Indeed, MN presence has been used as a biomarker of genotoxicity in animals [ 27 – 31 ] and vegetables [ 32 ].…”