Induction of metallothionein and stimulation of calcification by dexamethasone in cultured clonal osteogenic cells, MC3T3-E1

Miyahara, Tetsuhiro; Nemoto, Masaru; Tukamoto, Shinichi; Yamada, Hiroshi; Kono, Hiroshi; Kuze, Syougo; Sudo, Hiroko; Yamamoto, S.

doi:10.1016/0378-4274(91)90200-p

Cited by 14 publications

(7 citation statements)

References 24 publications

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“…7). We believe that osteoblasts are resistant to both heavy metals as a result of the production of MT (Miyahara et al, 1991;Liu et al, 2004;Dohi et al, 2005). These results coincided with those from a previous study, which showed that the osteoblastic activity did not change in cadmium-and methyl-mercury-treated goldfish scales until after 36 h of incubation (Suzuki et al, 2004).…”

Section: T B T a C O N T R O Lsupporting

confidence: 94%

Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts

Suzuki

Tabata

Kambegawa³

et al. 2006

Life Sciences

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Section: T B T a C O N T R O Lsupporting

confidence: 94%

Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts

Suzuki

Tabata

Kambegawa³

et al. 2006

Life Sciences

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“…MT-1 and MT-2 are acute-phase proteins co-induced in response to various agents such as heavy metals, glucocorticoids, oxidative stress, and cytokines, and their individual functions are not clear. Miyahara et al observed that undifferentiated osteoblasts synthesized MT on exposure to dexamethasone with enhanced calcification in the presence of β-glycerophosphate 22. Furthermore, Lin et al suggested that ZnCl 2 is able to promote dental pulp stem cells (DPSCs) differentiation by upregulating MT 23.…”

Section: Discussionmentioning

confidence: 99%

Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells

et al. 2012

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ObjectivesWe analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization.Materials and MethodsInduced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data.ResultsSix hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.ConclusionsGenes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.

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“…A mouse immature osteoblast-like cell line, MC 3T3-E1 (American Type Culture Collection, Manassas, VA), was used for this study, which could be further differentiated by osteogenic factors (Jadlowiec et al, 2004;Miyahara et al, 1991) and widely used for studies on biological effects of fluid flow (Jackson et al, 2006;Liu et al, 2007). The cells were subcultured in a culture medium consisting of a-minimum essential medium (a-MEM, Gibco BRL, Grand Island, NY), 1% penicillin-streptomycin, and 10% fetal bovine serum (FSB; Gibco BRL) in a 378C incubator with a humidified atmosphere of 95% air and 5% CO 2 .…”

Section: Cell Seeding and Culturementioning

confidence: 99%

3D culture of osteoblast‐like cells by unidirectional or oscillatory flow for bone tissue engineering

Furukawa

Ushida

2009

Biotech & Bioengineering

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A medium perfusion system is expected to be beneficial for three-dimensional (3D) culture of engineered bone, not only by chemotransport enhancement but also by mechanical stimulation. In this study, perfusion systems with either unidirectional or oscillatory medium flow were developed, and the effects of the different flow profiles on 3D culturing of engineered bone were studied. Mouse osteoblast-like MC 3T3-E1 cells were 3D-cultured with porous ceramic scaffolds in vitro for 6 days under static and hydrodynamic conditions with either a unidirectional or oscillatory flow. We found that, in the static culture, the cells proliferated only on the scaffold surfaces. In perfusion culture with the unidirectional flow, the proliferation was significantly higher than in the other groups but was very inhomogeneous, which made the construct unsuitable for transplantation. Only the oscillatory flow allowed osteogenic cells to proliferate uniformly throughout the scaffolds, and also increased the activity of alkaline phosphatase (ALP). These results suggested that oscillatory flow might be better than unidirectional flow for 3D construction of cell-seeded artificial bone. The oscillatory perfusion system could be a compact, safe, and efficient bioreactor for bone tissue engineering.

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Induction of metallothionein and stimulation of calcification by dexamethasone in cultured clonal osteogenic cells, MC3T3-E1

Cited by 14 publications

References 24 publications

Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts

Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts

Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells

3D culture of osteoblast‐like cells by unidirectional or oscillatory flow for bone tissue engineering

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