Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts

Suzuki, Nobuo; Tabata, M.; Kambegawa, Akira; Srivastav, Ajai K.; Shimada, Atsuko; Takeda, Hiroyuki; Kobayashi, Masaki; Wada, Shigehito; Katsumata, Toshiyuki; Hattori, Atsuhiko

doi:10.1016/j.lfs.2005.10.004

Cited by 36 publications

(29 citation statements)

References 72 publications

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“…However, no effect has been observed in blood calcium content of DDT-treated flounders Platichthys flesus (Haux 1979), methoxychlor-treated northern puffer Sphaeroides maculatus (Eisler 1967), cadmium-exposed Oncorhynchus niloticus (Oner et al 2008) and bifenthrin-treated rainbow trout Oncorhynchus mykiss (Velisek et al 2009). Contrary to the present investigation, few workers have noticed elevated calcium levels of fish after exposure to pesticides (Sharma et al 1982;Suzuki et al 2006). Heteropneustes fossilis when subjected to Nerium indicum leaf extract exposure showed a decrease in serum phosphate content.…”

Section: Discussioncontrasting

confidence: 99%

“…In vertebrates, calcium plays a vital role in a variety of biological processes. Although there exist several reports regarding the impact of toxicants on electrolyte composition of the blood (Suzuki et al 2006;Rai et al 2009;Velisek et al 2009;Srivastav et al 2010;Mishra et al 2010a, b), there exist no information regarding the impact of green pesticides on blood electrolytes of fish. Hence, it was aimed in the present investigation to study the toxic effect of a green pesticide Nerium indicum leaf extract on the freshwater teleost Heteropneustes fossilis in terms of serum calcium and phosphate levels.…”

Section: Introductionmentioning

confidence: 99%

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Alterations in blood electrolytes of a freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide, Nerium indicum leaf extract

Prasad

Kumar

Mishra

et al. 2010

Fish Physiol Biochem

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The present study aimed at investigating the effects of Nerium indicum leaf extract on the blood electrolytes of Heteropneustes fossilis for short- and long term. Fish were subjected to Nerium indicum leaf extract for short term (11.27 mg/L i.e. 0.8 of 96 h LC₅₀) and long term (2.81 mg/L i.e. 0.2 of 96 h LC₅₀). Fish were killed on each time intervals from control and experimental (Nerium indicum) groups after 24, 48, 72, and 96 h in short-term exposure and after 7, 14, 21, and 28 days in long-term experiment. Blood samples were analyzed for calcium and inorganic phosphate levels. Acute exposure of Nerium indicum leaf extract caused a progressive decrease in the serum calcium levels after 48 h in fish H. fossilis, which persists till the close of the experiment (96 h). The serum inorganic phosphate levels remain unaffected till 48 h in the Nerium indicum leaf extract-exposed fish. After 72 and 96 h, the levels exhibit a decrease. Chronic Nerium indicum leaf extract treatment provoked a decrease in serum calcium levels at day 14. This decrease continues till 28 days. The serum phosphate level of the Nerium indicum leaf extract-treated fish decreases on day 14 and 21. However, on day 28, the levels become close to the normal values. We conclude that Nerium indicum leaf extract exposure alters the blood electrolytes of the fish, thus causing physiological disturbances which might affect seriously the normal vital functions, growth rate, reproduction, and their survival in nature.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alterations in blood electrolytes of a freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide, Nerium indicum leaf extract

Prasad

Kumar

Mishra

et al. 2010

Fish Physiol Biochem

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“…The enzyme activity in one scale can be detected by transferring it into a 96-well microplate and directly incubating it with the substrate in each well. Using this in vitro system, the effects of endocrine disrupters-such as bisphenol-A (Suzuki and Hattori, 2003), tributyltin (Suzuki et al, 2006), and polychlorinated biphenyl (Yachiguchi et al, 2014a)-and heavy metals (i.e., cadmium and organic mercury) (Suzuki et al, 2004;Suzuki et al, 2011b;Yachiguchi et al, 2014a) M) (Yachiguchi et al, 2014b). In the present study, we can sensitively evaluate the effect of seawater polluted by PAHs and NPAHs on osteoblasts for short incubation times.…”

Section: Discussionmentioning

confidence: 87%

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish,Carassius auratus

Suzuki¹,

Sato²,

Nassar³

et al. 2016

Zoological Science

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We have developed an original in vitro bioassay using teleost scale that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from 2 sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We can detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added into culture medium at dilution rates of 50, 100, and 500 and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. As a result, ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, although TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-B ligand) decreased significantly, as did the ALP enzyme activity. Actually, ALP activity decreased with selected PAHs and NPAHs treatments. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influenced bone metabolism in teleosts.

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“…Trisubstituted organotins, tributyltin (TBT) and triphenyltin, have been shown to interfere with biomineralization in different species [Chagot et al, 1990;Adeeko et al, 2003;Tsukamoto et al, 2004;Suzuki et al, 2006;Sarpa et al, 2007;Salmela et al, 2008]. Of the organotins, TBT is estimated to cause the main risk for humans [Risk and Policy Analysts Ltd., 2005].…”

Section: Introductionmentioning

confidence: 99%

“…TBT has been shown to impair bone mineralization and to have adverse effects on osteoblast differentiation and activity, and calcium deposition in vivo and in vitro [Tsukamoto et al, 2004;Suzuki et al, 2006;Sarpa et al, 2007]. Furthermore, Tsukamoto et al [2004] observed that TBT decreases the expression of genes encoding tissue nonspecific alkaline phosphatase (Alpl) and osteocalcin (Ocn), both associated with mineralization, and inhibits Alpl activity in rat calvarial osteoblast-like cells.…”

Section: Introductionmentioning

confidence: 99%

Tributyltin Alters Osteocalcin, Matrix Metalloproteinase 20 and Dentin Sialophosphoprotein Gene Expression in Mineralizing Mouse Embryonic Tooth in vitro

Salmela

Alaluusua²,

Sahlberg³

et al. 2011

Cells Tissues Organs

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We showed in a previous in vitro study that tributyltin (TBT) arrests dentin mineralization and enamel formation in developing mouse tooth. The present aim was to investigate the effect of TBT on the expression of genes associated with mineralization of dental hard tissues. Embryonic day 18 mouse mandibular first molars were cultured for 3, 5 or 7 days and exposed to 1.0 µM TBT and studied by real-time quantitative polymerase chain reaction (RT-QPCR) for the expressions of osteocalcin (Ocn), alkaline phosphatase (Alpl), dentin matrix protein 1 (Dmp1), dentin sialophosphoprotein (Dspp) and matrix metalloproteinase 20 (Mmp-20).Ocn, Mmp-20 and Dspp, whose expressions showed changes in RT- QPCR, were further analyzed by in situ hybridization of tissue sections. In situ hybridization showed that TBT decreased Ocn expression in odontoblasts but increased the expression in the epithelial tooth compartment. In QPCR assays, the net effect in the whole tooth was increased expression. TBT also reduced Mmp-20 expression in ameloblasts and odontoblasts. Dspp expression varied but both QPCR assays and in situ hybridization showed a decreasing trend. TBT exposure had no clear effect on Alpl and Dmp1 expressions. Increased Ocn expression by epithelial enamel organ may inhibit dentin mineralization and enamel formation. Decreased Ocn, Mmp-20 and Dspp expressions in odontoblasts may indicate delayed cell differentiation, or TBT may specifically decrease the expression of genes involved in dentin mineralization. While decreased Mmp-20 expression by TBT in ameloblasts may impair enamel mineralization, the coincident reduction in Mmp-20 and Dspp expressions in odontoblasts may potentiate the delay of dentin mineralization.

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Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts

Cited by 36 publications

References 72 publications

Alterations in blood electrolytes of a freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide, Nerium indicum leaf extract

Alterations in blood electrolytes of a freshwater catfish Heteropneustes fossilis in response to treatment with a botanical pesticide, Nerium indicum leaf extract

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish,Carassius auratus

Tributyltin Alters Osteocalcin, Matrix Metalloproteinase 20 and Dentin Sialophosphoprotein Gene Expression in Mineralizing Mouse Embryonic Tooth in vitro

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