Induction of members of the Fos/Jun family of immediate-early genes in identified hypothalamic neurons: in vivo evidence for differential regulation

Luckman, Simon M.; Dye, S.; Cox, Hilary J.

doi:10.1016/0306-4522(96)00076-0

Cited by 39 publications

(26 citation statements)

References 42 publications

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“…shown to be upregulated in the SON after dehydration (Carter and Murphy, 1990;Sharp et al, 1991;Luckman et al, 1996;Miyata et al, 2001b;Cunningham et al, 2004;Ji et al, 2005;Kawasaki et al, 2005;Penny et al, 2005;Gottlieb et al, 2006). Here we identify JunD as also being upregulated (supplemental Table 3, available at www.jneurosci.org as supplemental material), an observation that we confirmed by in situ hybridization and immunocytochemistry (Yao et al, unpublished observation).…”

Section: Discussionsupporting

confidence: 80%

Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A

Qiu¹,

Yao²,

Hindmarch³

et al. 2007

J. Neurosci.

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The supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamo-neurohypophyseal system (HNS) undergo a dramatic function-related plasticity during dehydration. We hypothesize that alterations in steady-state transcript levels might be partially responsible for this remodeling. In turn, regulation of transcript abundance might be mediated by transcription factors. We used microarrays to identify changes in the expression of mRNAs encoding transcription factors in response to water deprivation in the SON. We observed downregulation of 10 and upregulation of 28 transcription factor transcripts. For five of the upregulated mRNAs, namely gonadotropin inducible ovarian transcription factor 1 (Giot1), Giot2, cAMP-responsive element binding protein 3-like 1, CCAAT/enhancer binding protein ␤, and activating transcription factor 4, in situ hybridization was used to confirm the array results, demonstrating a significant increase in expression in SON and PVN magnocellular neurons (MCNs) after 3 d of water deprivation and, in some cases, upregulation in parvocellular PVN neurons. Using a viral vector expressing a potent inhibitor of cAMP-dependent protein kinase A (PKA), we show that the osmotically induced increase in the abundance of transcripts encoding Giot1 is mediated in vivo by the PKA pathway. We thus suggest that signaling pathways activated by dehydration in MCNs mediate transcription factor gene activation, which, in turn, regulate target genes that mediate HNS remodeling.

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Section: Discussionsupporting

confidence: 80%

Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A

Qiu¹,

Yao²,

Hindmarch³

et al. 2007

J. Neurosci.

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“…An absence of Fos in most ER␣ϩ/PjnϪ, ER␣Ϫ/Pjnϩ, and ER␣ϩ/Pjnϩ neurons does not necessarily indicate inactivity or unresponsiveness (10). First, neurons may be activated below the threshold necessary for Fos induction (52). Second, neurons may not express Fos despite being highly stimulated (53).…”

Section: Discussionmentioning

confidence: 98%

Hormonal-Neural Integration in the Female Rat Ventromedial Hypothalamus: Triple Labeling for Estrogen Receptor-α, Retrograde Tract Tracing from the Periaqueductal Gray, and Mating-Induced Fos Expression

Calizo

Flanagan‐Cato

2003

Endocrinology

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The lordosis reflex, a stereotypic posture adopted by female rats during sexual behavior, requires the convergence of a hormonal signal, estrogen, with a descending neural pathway from the ventromedial hypothalamic nucleus (VMH). The VMH contains at least three lordosis-relevant neural populations: estrogen receptor-alpha immunoreactive (ERalpha-IR) neurons, VMH neurons that project to the periaqueductal gray (PAG), and neurons that are ERalpha-IR and project to the PAG. Expression of Fos, a marker for neuronal activation, is increased in the VMH after mating. However, it is unknown which, if any, of these lordosis-relevant populations is activated. The majority of ERalpha-IR and projection neurons were not colocalized. Of the Fos-positive neurons, 41% neither contained ERalpha nor projected to the PAG, and 35% contained ERalpha but did not project to the PAG. Only 25% of Fos-positive neurons projected to the PAG, including projection neurons that expressed ERalpha. Our results suggest that mating activates several distinct VMH neuron types. However, ERalpha-IR neurons are activated to a greater extent compared with the PAG-projecting neurons.

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“…Application of cAMP agonists to in vitro cultures of fetal hypothalamus results in increased VP RNA levels, (31,32) and both the bovine (55) and rat (56) VP promoters respond positively to cAMP after transfection into cultured cells. Osmotic stimulation leads to the rapid phosphorylation of the cAMP response element binding protein (CREB) within the SON and PVN, (57) as well as the induction of mRNAs encoding members of the Fos/Jun family of transcription factors, (58) including c-fos, (59) which is known to be cAMP responsive through a number of cAMP response elements. (60) These transcription factors bind as dimers to the AP-1 DNA consensus sequence; dimers containing Fos B exhibit the highest DNA binding affinity and have the strongest transcriptional activity.…”

Section: Vp Gene Expression In Transgenic Ratsmentioning

confidence: 99%

Something fishy in the rat brain: molecular genetics of the hypothalamo-neurohypophysial system

et al. 1998

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Induction of members of the Fos/Jun family of immediate-early genes in identified hypothalamic neurons: in vivo evidence for differential regulation

Cited by 39 publications

References 42 publications

Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A

Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A

Hormonal-Neural Integration in the Female Rat Ventromedial Hypothalamus: Triple Labeling for Estrogen Receptor-α, Retrograde Tract Tracing from the Periaqueductal Gray, and Mating-Induced Fos Expression

Something fishy in the rat brain: molecular genetics of the hypothalamo-neurohypophysial system

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