Induction of lung-like cells from mouse embryonic stem cells by decellularized lung matrix

Kawai, Norikazu; Ouji, Yukiteru; Sakagami, Masaharu; Terauchi, Takashi; Sawabata, Noriyoshi; Yoshikawa, Masahide; Taniguchi, Shigeki

doi:10.1016/j.bbrep.2018.06.005

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…[90] These approaches provide unmatched pulmonary biomimetic geometry and an active ECM endowed with cellular responsiveness, thus being capable of guiding cell attachment and behavior, as well as supporting progenitor cell differentiation. [91,92] Decellularized lung ECM may also be further processed into other scaffold forms, such as hydrogels, [93,94] which can similarly be used to study lung cell-ECM interactions in vitro, or small 3D-fragments with preserved vasculature and airway structure, suitable for high-throughput analysis. [95] Relevantly, a number of reports have demonstrated that healthy and aged or diseased lungs respond to decellularization protocols differently and result in disparate cellular behaviors, [96][97][98][99] representing potentially useful models to study lung pathophysiology.…”

Section: Decellularized Ecm and Scaffold-based Modelsmentioning

confidence: 99%

Advanced In Vitro Lung Models for Drug and Toxicity Screening: The Promising Role of Induced Pluripotent Stem Cells

Moreira¹,

Müller

Costa³

et al. 2021

Advanced Biology

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Respiratory diseases are among the global leading causes of morbidity and mortality. Acute conditions arising from infection, such as pneumonia and tuberculosis, affect millions of people worldwide, the latter being the most common lethal infectious disease with 1.4 million annual deaths. [1] Upon infection, the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which caused a worldwide pandemic, leads to the clinical picture of the coronavirus disease-2019 (COVID-19) with possibly severe and life-threatening progression. Lung cancer is the most frequently diagnosed malignancy and the main cause of cancer-related death, [2] with markedly low survival rates especially when the diagnosis is performed at an advanced state of the disease. [3] Moreover, chronic respiratory diseases (CRDs), including chronic obstructive pulmonary disease (COPD), asthma, and interstitial lung disease (ILD), have consistently received less attention in comparison to other non-communicable diseases, continuing to exert a considerable socioeconomic impact. [4,5] Risk factors for the development of respiratory diseases include, for example, tobacco use and second-hand smoke, as well as exposure to air-pollutants, which has been accentuated with the widespread use of fossil fuels. [4] It is clear that a great number of these risks can be mitigated by lifestyle changes, promoted by anti-tobacco campaigns already in place at a global scale and by the use of renewable, clean energy sources, and two recent studies indicate that the age-standardized incidence and prevalence of CRDs has decreased from 1990 to 2017. [4,5] However, the risk of developing CRDs, particularly COPD, appears to increase steeply with age; [4,5] most strikingly, these diseases were the third leading cause of death in 2017 [4] and potentially in 2020. [6] In addition to microbial, environmental, and lifestyle-related factors, a large number of lung diseases have a genetic origin. [7] Cystic fibrosis (CF), for example, is an incurable hereditary disorder known to originate from different mutations in the gene coding for the CF transmembrane conductance regulator (CFTR), an ion transporter, resulting in severe alterations in pulmonary physiology that culminate in impaired lung function, respiratory distress and, ultimately, death. [8,9]

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Section: Decellularized Ecm and Scaffold-based Modelsmentioning

confidence: 99%

Advanced In Vitro Lung Models for Drug and Toxicity Screening: The Promising Role of Induced Pluripotent Stem Cells

Moreira¹,

Müller

Costa³

et al. 2021

Advanced Biology

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“…The lung, however, develops in response to chemical signals within a highly dynamic mechanical environment of cyclic strain, pressure and a complex branching tubular architecture [ 169 ]. Indeed, it is well established that mechanical cues can impact progenitor cell fate [ 102 , [170] , [171] , [172] , [173] , [174] , [175] , [176] , [177] , [178] , [179] , [180] ] and emerging evidence suggests that the mechanical environment can be manipulated to produce predictable fate choices in stem and progenitor cells [ [181] , [182] , [183] , [184] ]. For example, the importance of biophysical manipulation associated with tissue structure was beautifully articulated by von Erlach et al ., who described a mechanistic link between cell geometry and lipid rafts within plasma membranes that play a key role in cell signalling and therefore, cell fate.…”

Section: Opportunities To Exploit Mechanical Cues For Improving Direcmentioning

confidence: 99%

Current strategies and opportunities to manufacture cells for modeling human lungs

Varma

Soleas

Waddell

et al. 2020

Advanced Drug Delivery Reviews

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Chronic lung diseases remain major healthcare burdens, for which the only curative treatment is lung transplantation. In vitro human models are promising platforms for identifying and testing novel compounds to potentially decrease this burden. Directed differentiation of pluripotent stem cells is an important strategy to generate lung cells to create such models. Current lung directed differentiation protocols are limited as they do not 1) recapitulate the diversity of respiratory epithelium, 2) generate consistent or sufficient cell numbers for drug discovery platforms, and 3) establish the histologic tissue-level organization critical for modeling lung function. In this review, we describe how lung development has formed the basis for directed differentiation protocols, and discuss the utility of available protocols for lung epithelial cell generation and drug development. We further highlight tissue engineering strategies for manipulating biophysical signals during directed differentiation such that future protocols can recapitulate both chemical and physical cues present during lung development.

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“…Decellularization has been appeared as promising approach in lung tissue engineering which this method can relatively maintain the extracellular matrix which help the cells for proliferation, migration, and differentiation. And, there is a believe that decellularization approaches may produce potential scaffolds to induce ES cells and iPS cells to differentiate lung cells (Kawai et al, 2018). Kawai et al studied the ability of decellularized lung scaffolds in ES cells differentiation into different kinds of lung cells.…”

Section: Differentiationmentioning

confidence: 99%

Lung tissue engineering: An update

Tebyanian

Karami

Nourani

et al. 2019

Journal Cellular Physiology

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Pulmonary disease is a worldwide public health problem that reduces the life quality and increases the need for hospital admissions as well as the risk of premature death.A common problem is the significant shortage of lungs for transplantation as well as patients must also take immunosuppressive drugs for the rest of their lives to keep the immune system from attacking transplanted organs. Recently, a new strategy has been proposed in the cellular engineering of lung tissue as decellularization approaches. The main components for the lung tissue engineering are: (1) A suitable biological or synthetic three-dimensional (3D) scaffold, (2) source of stem cells or cells, (3) growth factors required to drive cell differentiation and proliferation, and (4) bioreactor, a system that supports a 3D composite biologically active. Although a number of synthetic as well biological 3D scaffold suggested for lung tissue engineering, the current favorite scaffold is decellularized extracellular matrix scaffold. There are a large number of commercial and academic made bioreactors, the favor has been, the one easy to sterilize, physiologically stimuli and support active cell growth as well as clinically translational. The challenges would be to develop a functional lung will depend on the endothelialized microvascular network and alveolar-capillary surface area to exchange gas. A critical review of the each components of lung tissue engineering is presented, following an appraisal of the literature in the last 5 years. This is a multibillion dollar industry and consider unmet clinical need. K E Y W O R D S in vivo, lung, scaffold, stem cell, tissue engineering

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Induction of lung-like cells from mouse embryonic stem cells by decellularized lung matrix

Cited by 8 publications

References 40 publications

Advanced In Vitro Lung Models for Drug and Toxicity Screening: The Promising Role of Induced Pluripotent Stem Cells

Advanced In Vitro Lung Models for Drug and Toxicity Screening: The Promising Role of Induced Pluripotent Stem Cells

Current strategies and opportunities to manufacture cells for modeling human lungs

Lung tissue engineering: An update

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