Lung tissue engineering: An update

Tebyanian, Hamid; Karami, Ali; Nourani, Mohammad Reza; Motavallian, Ebrahim; Barkhordari, Aref; Yazdanian, Mohsen; Seifalian, Alexander M.

doi:10.1002/jcp.28558

Cited by 33 publications

(22 citation statements)

References 91 publications

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“…In recent decades, whole lung engineering has been developing as a promising approach in the field of pulmonary regenerative medicine 11,25,26 . Advances have been made in the utilization of decellularized lung scaffolds in organ replacement and regeneration.…”

Section: Discussionmentioning

confidence: 99%

Decellularization of porcine whole lung to obtain a clinical‐scale bioengineered scaffold

et al. 2021

J Biomedical Materials Res

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Whole‐organ engineering is emerging as an alternative source for xenotransplantation in end‐stage diseases. Utilization of decellularized whole lung scaffolds created by detergent perfusion is an effective strategy for organ replacement. In the current study, we attempted to decellularize porcine whole lungs to generate an optimal and reproducible decellularized matrix for future clinical use. Porcine whole lungs were decellularized via perfusion of various detergents (sodium dodecyl sulfate (SDS)/Triton X‐100, sodium lauryl ether sulfate (SLES)/Triton X‐100, dextrose/SDS/Triton X‐100 and dextrose/SLES/Triton X‐100) through the pulmonary artery and bronchus of the lung. The decellularized scaffolds were evaluated for decellularization efficiency, extracellular matrix (ECM) component preservation, xenoantigen removal and compatibility. The resulting lung scaffolds obtained from treatment with the dextrose/SLES/Triton X‐100 cocktail showed minimal residual cellular components and xenoantigens, including DNA and protein, and good preservation of ECM composition. Evaluation of the porcine lung ECM by specific staining and immunofluorescence confirmed that the three‐dimensional ultrastructure of the ECM was noticeably preserved in the SLES‐treated groups. In addition, the decellularized lung scaffolds originating from the dextrose/SLES/Triton X‐100 cocktail supported cell adhesion and growth. In summary, the novel detergent SLES alleviated the damage to retain a better‐preserved ECM than SDS. Sequential Triton X‐100 perfusion eliminated SLES. Moreover, performing dextrose perfusion in advance further protected scaffold components, especially collagen. We developed an optimal dextrose/SLES/Triton X‐100 cocktail method that can be used for the decellularization of porcine whole lung to obtain a clinical‐scale bioengineered scaffold.

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Section: Discussionmentioning

confidence: 99%

Decellularization of porcine whole lung to obtain a clinical‐scale bioengineered scaffold

et al. 2021

J Biomedical Materials Res

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“…To create hybrid materials for tissue engineering of the lungs, one must have a good understanding of the anatomy and physiology of this organ [175]. Anatomically, the lungs are divided into two sections-the proximal and distal airways.…”

Section: Hybrid Biomaterials Applied For Lungsmentioning

confidence: 99%

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

2020

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In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorganic content, hybrid materials allow to mimic properties of various types of harder tissues. That leads to the concept of "one-matches-all" referring to materials possessing the same polymeric base, but different inorganic content to enable tissue growth and repair, proliferation of cells, and the formation of the ECM (extra cellular matrix). Furthermore, adding drug delivery carriers to coatings and scaffolds designed with such materials brings additional functionality by encapsulating active molecules, antibacterial agents, and growth factors. We discuss here materials and methods of their assembly from a general perspective together with their applications in various tissue engineering sub-areas: interstitial, connective, vascular, nervous, visceral and musculoskeletal tissues. The overall aims of this review are two-fold: (a) to describe the needs and opportunities in the field of bio-medicine, which should be useful for material scientists, and (b) to present capabilities and resources available in the area of materials, which should be of interest for biologists and medical doctors.Polymers 2020, 12, 620 2 of 30 allows to tailor functionalize the coatings, where their responsiveness to external stimuli is one of their biggest advantages.Polymers stand out as a special class of materials due to the flexibility of design of their blocks and various functionalities, which is brought by their versatile assembly or by introducing additional side-groups or blocks, in the case of block-co-polymers. In this regard, both synthetic and biocompatible polymers are available, and polymer engineering represents a growing area tailoring the needs often driven by applications. With all advantages and the flexibility of the design, there is one significant weakness of polymers-the softness. In regard with tissue engineering, that translates to the fact that some materials can match predominantly soft tissue, but it is difficult to tune mechanical properties of polymers to match the hardness of bones, tendons, etc. And since mechanical properties of materials affect and even drive cell and tissue growth, enhancement of mechanical properties of polymers and biomaterials is essential. Chemical crosslinking can be applied to address these challenges, but that solves problems only partially.To solve these challenges the so-called hybrid materials [6] are used, which possess both inorganic and organic contents. Increasingly, hybrid materials are used in designing the extracellular matrix. The hybrid matrix design for various tissue engineering applications should meet bio-medical requirements. On the one hand, it needs to be biocompatible and biodegradable, which is achieved through the po...

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“…Transplantation of lung suffers the shortage of donors as well as inevitable immunosuppressive drugs for patients. Lung tissue engineering shows an alternative to relieve the problems of shortage of lung transplantation (Tebyanian et al 2019 ) and is beneficial to the recovery of patients’ lung. Biological scaffolds made by three-dimensional printing technology combining regenerative cell population is an attracting candidate strategy for lung tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

“…For example, decellularized scaffolds from human or animals, collagen type I-GAG, gelatin, polyglycolic acid (PGA), poly-DL-lactic acid (PDLLA), poly lactic-co-glycolic acid (PLGA), polylactic acid (PLLA), Pluronic F-127 etc. (Tebyanian et al 2019 ; Wagner et al 2014 ) However, synthetic polymers sometimes fail to drive the differentiation of cultured cells (Lin et al 2006 ; Mondrinos et al 2006 ). In contrast, natural biomaterials are promising for constructing biomimetic extracellular matrix (ECM) for ex-vivo research of lung tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

et al. 2020

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A novel biomaterial ink consisting of regenerated silk fibroin (SF) and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose (OBC) nanofibrils was developed for 3D printing lung tissue scaffold. Silk fibroin backbones were cross-linked using horseradish peroxide/H 2 O 2 to form printed hydrogel scaffolds. OBC with a concentration of 7wt% increased the viscosity of inks during the printing process and further improved the shape fidelity of the scaffolds. Rheological measurements and image analyses were performed to evaluate inks printability and print shape fidelity. Three-dimensional construct with ten layers could be printed with ink of 1SF-2OBC (SF/OBC = 1/2, w/w). The composite hydrogel of 1SF-1OBC (SF/OBC = 1/1, w/w) printed at 25 °C exhibited a significantly improved compressive strength of 267 ± 13 kPa and a compressive stiffness of 325 ± 14 kPa at 30% strain, respectively. The optimized printing parameters for 1SF-1OBC were 0.3 bar of printing pressure, 45 mm/s of printing speed and 410 μm of nozzle diameter. Furthermore, OBC nanofibrils could be induced to align along the print lines over 60% degree of orientation, which were analyzed by SEM and X-ray diffraction. The orientation of OBC nanofibrils along print lines provided physical cues for guiding the orientation of lung epithelial stem cells, which maintained the ability to proliferate and kept epithelial phenotype after 7 days’ culture. The 3D printed SF-OBC scaffolds are promising for applications in lung tissue engineering. Electronic supplementary material The online version of this article (10.1007/s10570-020-03526-7) contains supplementary material, which is available to authorized users.

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Lung tissue engineering: An update

Cited by 33 publications

References 91 publications

Decellularization of porcine whole lung to obtain a clinical‐scale bioengineered scaffold

Decellularization of porcine whole lung to obtain a clinical‐scale bioengineered scaffold

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

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