Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria

Greenberg, E. Peter; Hastings, J. Woodland; Ulitzur, S.

doi:10.1007/bf00409093

Cited by 335 publications

(245 citation statements)

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“…Because V. harveyi is found in marine environments inhabited by many other species of bacteria, it is possible that V. harveyi monitors its environment for signals produced by other species of bacteria and produces light in response to these interspecies stimuli (6). We found that besides V. harveyi, only one species, V. parahaemolyticus, produced an AI-1-like activity, indicating that V. harveyi system 1 is highly specific.…”

mentioning

confidence: 75%

Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi

1997

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Different species of bacteria were tested for production of extracellular autoinducer-like activities that could stimulate the expression of the luminescence genes in Vibrio harveyi. Several species of bacteria, including the pathogens Vibrio cholerae and Vibrio parahaemolyticus, were found to produce such activities. Possible physiological roles for the two V. harveyi detection-response systems and their joint regulation are discussed.At least two species of marine bacteria, Vibrio fischeri and Vibrio harveyi, express bioluminescence in response to cell density. These two vibrios are found in different environments in the ocean. V. harveyi is found free-living in the sea as well as in the gut tracts of marine animals, where it exists at high population densities in association with other species of bacteria. V. fischeri is found in these habitats and also lives in pure culture as a light organ symbiont of various fish and squid (8). V. fischeri and V. harveyi accomplish density-dependent lux regulation by the synthesis, excretion, and detection of small signal molecules called autoinducers, which accumulate in the environment (9). The autoinducer that controls light production in V. fischeri is N-(3-oxohexanoyl)-L-homoserine lactone. Two autoinducers control density-dependent lux expression in V. harveyi. One of the V. harveyi autoinducers is N-(3-hydroxybutanoyl)-L-homoserine lactone (AI-1), and the second autoinducer (AI-2) remains to be identified. Recently, a number of other bacteria have been shown to control cell density-dependent functions through the excretion of and response to acyl-homoserine lactone autoinducers. Cell density-dependent regulation of lux expression is an example of a phenomenon called quorum sensing (5).Genetic analysis of the density-sensing apparatus of V. harveyi has shown that two independent density-sensing systems exist, and each is composed of a sensor-autoinducer pair; system 1 is composed of sensor 1 and AI-1, and system 2 is composed of sensor 2 and AI-2 (1, 2). The two density-sensing systems are redundant, because a null mutation in either system alone results in Lux ϩ strains, whereas null mutations in both systems render the cells dark and incapable of density sensing. In 1979 Greenberg et al. (6) reported that V. harveyi was stimulated to produce light following the addition of cellfree culture fluid from several species of nonluminous bacteria. At the time of that study it was not known that two autoinducer-detection systems existed in V. harveyi. Because V. harveyi mutants capable of responding to only AI-1 or AI-2 now exist, it is possible to determine through which pathway(s) the signals from these other organisms flow. Now that we also appreciate that many bacteria communicate intercellularly and control gene expression through the use of autoinducers, it is of interest to understand how they might accomplish crossspecies communication and use it for survival in various niches. In this study we have used V. harveyi sensor mutants as reporters for specific autoinduc...

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confidence: 75%

Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi

1997

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“…The strains used for the AI-2 bioassay, Vibrio harveyi BB170 (HAI-1 negative; AI-2 positive; CAI-1 positive), V. harveyi JMH597 (HAI-1 negative; AI-2 positive; CAI-1 negative) [30], and V. harveyi JAF548, in which bioluminescence is not under the control of QS systems due to the permanent activation of the QS-dependent bioluminescence repressor LuxO [31,32], were cultured in LB supplemented with 30 μg ml −1 of the antibiotic kanamycin (LB+Km) and in autoinducer bioassay medium (AB) [33]. …”

Section: Methodsmentioning

confidence: 99%

Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

Muras

Mayer

Romero

et al. 2018

Journal of Oral Microbiology

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Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method. Results: The addition of the cell extract from Tenacibaculum sp. 20J reduced biofilm formation in S. mutans ATCC25175 by 40–50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in S. oralis DSM20627 and S. dentisani 7747 biofilms. Conclusions: The ability of Tenacibaculum sp. 20J to interfere with AI-2 and inhibit biofilm formation in S. mutans was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required.

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“…Luria-Bertani medium (LB) contained 10 g bacto tryptone (Difco), 5 g yeast extract (Difco), and 10 g NaCl per liter (16). The recipe for autoinducer bioassay (AB) medium has been reported (17). Where specified, glucose was added from a sterile 20% stock to a final concentration of 0.5%.…”

Section: Methodsmentioning

confidence: 99%

Quorum sensing in Escherichia coli , Salmonella typhimurium , and Vibrio harveyi : A new family of genes responsible for autoinducer production

Surette

Miller²,

Bassler

1999

Proc. Natl. Acad. Sci. U.S.A.

872

691

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In bacteria, the regulation of gene expression in response to changes in cell density is called quorum sensing. Quorum-sensing bacteria produce, release, and respond to hormone-like molecules (autoinducers) that accumulate in the external environment as the cell population grows. In the marine bacterium Vibrio harveyi two parallel quorum-sensing systems exist, and each is composed of a sensor-autoinducer pair. V. harveyi reporter strains capable of detecting only autoinducer 1 (AI-1) or autoinducer 2 (AI-2) have been constructed and used to show that many species of bacteria, including Escherichia coli MG1655, E. coli O157:H7, Salmonella typhimurium 14028, and S. typhimurium LT2 produce autoinducers similar or identical to the V. harveyi system 2 autoinducer AI-2. However, the domesticated laboratory strain E. coli DH5␣ does not produce this signal molecule. Here we report the identification and analysis of the gene responsible for AI-2 production in V. harveyi, S. typhimurium, and E. coli. The genes, which we have named luxS V.h. , luxS S.t. , and luxS E.c. respectively, are highly homologous to one another but not to any other identified gene. E. coli DH5␣ can be complemented to AI-2 production by the introduction of the luxS gene from V. harveyi or E. coli O157:H7. Analysis of the E. coli DH5␣ luxS E.c. gene shows that it contains a frameshift mutation resulting in premature truncation of the LuxS E.c. protein. Our results indicate that the luxS genes define a new family of autoinducer-production genes.

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Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria

Cited by 335 publications

References 14 publications

Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi

Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi

Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

Quorum sensing in Escherichia coli , Salmonella typhimurium , and Vibrio harveyi : A new family of genes responsible for autoinducer production

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