Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

Abstract

Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method. Results: The addition of the cell extract from Tenacibaculum sp. 20J reduced biofilm formation in S. mutans ATCC25175 by 40–50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in S. oralis DSM20627 and S. dentisani 7747 biofilms. Conclusions: The ability of Tenacibaculum sp. 20J to interfere with AI-2 and inhibit biofilm formation in S. mutans was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required.

Microbiome Profiling Reveals a Microbial Dysbiosis During a Natural Outbreak of Tenacibaculosis (Yellow Mouth) in Atlantic Salmon

“…strain 20J have demonstrated tha this species contains a novel acyl homoserine lactone (AHL) that has broad-spectrum quorum quenching activity against virulent Escherichia coli (Mayer et al, 2015). Purified or crude cell extracts from the same species were also able to reduce biofilm formation of Streptococcus mutans (Muras et al, 2018) and inhibit the production of AHL by the fish pathogen Edwardsiella tarda (Romero et al, 2014). The ability of the dominant T. maritimum strains described herein to competitively inhibit other taxa within the oral cavity requires further targeted investigation.…”

Integrated Succinylome and Metabolome Profiling Reveals Crucial Role of S-Ribosylhomocysteine Lyase in Quorum Sensing and Metabolism of Aeromonas hydrophila*

“…AI-2 Activity Assay-AI-2 production was measured by site-directed mutagenesis of luxS using the Vibrio harveyi BB170 bioluminescence reporter assay, as previously described (41). All the strains were cultured overnight and cells were pelleted by centrifugation at 12,000 ϫ g for 5 min, followed by filtration of the supernatant through a 0.22-m sterile filter (Millipore).…”

Multiple Quorum Quenching Enzymes Are Active in the Nosocomial Pathogen Acinetobacter baumannii ATCC17978

“…The QQ enzyme Aii20J (Mayer et al, 2015 ) was added with every medium exchange at a concentration of 20 μg/mL. For each condition two coverslips were stained with crystal-violet (Muras et al, 2018b ) and another two were stained with LIVE/DEAD® BacLight™ Bacterial Viability Kit (Invitrogen) and observed with a Leica TCS SP5 X confocal laser scanning microscope (Leica Microsystem Heidelberg GmbH, Mannheim, Germany) with an HC PL APO 10 × /0.4 CS objective.…”