Induction of Leptomeningeal Cells Modification Via Intracisternal Injection

A Widespread Neurogenic Potential of Neocortical Astrocytes Is Induced by Injury

“…We asked whether VLMCs may contribute to the observed cortical neurogenesis in injured Rbpj-κ fl/fl mice. For this purpose, we achieved selective targeting of VLMCs through intracisternal delivery of endoxifen ( Zamboni et al., 2020 ) and subjected the mice to stab wound injury ( Figures 2 B and 2H–2J). The lesioned cortices of these animals were devoid of Ascl1 + or Dcx + neural progenitor cells, suggesting that VLMCs do not have neurogenic potential (n = 5; Figure 2 K).…”

“…For local recombination of cortical astrocytes, 0.5 μL of Gfap-Cre adenoassociated virus (> 10 13 GC) was injected using a 36G blunt needle (World Precision Instruments, WPI) mounted on a micromanipulator, and infused at 100 nl/min (+0.5 mm antero-posterior, +2.5 mm medio-lateral from Bregma, and −0.5 mm deep from dura). For recombination of leptomeningeal, endoxifen (10 μl, 0.5 mg/ml) was injected manually into the cisterna magna using Hamilton syringes with a 30G beveled needle bent to 30° at 3 mm from the tip ( Zamboni et al., 2020 ). Finally, recombination of cells at, and around, the subventricular zone was achieved through intraventricular injection of endoxifen (2 μl, 0.5 mg/ml) at −0.2 mm antero-posterior, +1 mm medio-lateral from Bregma, and −1.75 mm deep from dura, and delivered using a 33G beveled needle (World Precision Instruments, WPI) mounted onto a micromanipulator.…”