Induction of Lens Fibre Differentiation by Acidic and Basic Fibroblast Growth Factor (FGF)

Chamberlain, Coral G.; McAvoy, John W.

doi:10.3109/08977198909029122

Cited by 142 publications

(67 citation statements)

References 33 publications

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“…3), while aggregates containing higher numbers of 100,000 to 500,000 cells were not stable and demonstrated partial dissociation of cells into the surrounding gel. The generated lenslike structures demonstrated similar characteristics to lentoids reported in previously established culture systems, 6,[9][10][11][12] including an overall rounded appearance with diffuse transparency throughout that increased mainly within the outer periphery over the 26 day culture period. In addition, different-size MLE aggregates of 5000, 35,000, and 50,000 cells demonstrated a volume reduction to proportional half of the original volume at the end of the culture period.…”

Section: Matrigel Promotes Lentoid Formation Of Mle Aggregatessupporting

confidence: 62%

“…The observed nuclear loss along with an increase in bB1-crystallin and a decrease in a-SMA expression supports aggregate internal lens fiber differentiation that might lead to a tighter lens fiber cell packing and according to volume loss in our system. 21,27 When MLE aggregates were cultured in the presence of lens fiber differentiation supporting growth factor bFGF, 10,11,[28][29][30] the aggregate structures demonstrated an initial increase in aggregate size at 1-week bFGF treatment that seemed to be based on the addition of outer peripheral …”

Section: Discussionmentioning

confidence: 99%

“…The higher reduction of aSMA expression in the presence of bFGF matches the role of this growth factor in lens fiber formation and suggests increased appearance of lens fiber differentiation under these culture conditions. 2,10,11 …”

Section: Changes In Mle Aggregate Bb1-crystallin Expression In Presenmentioning

confidence: 99%

“…Increased MLE aggregate size and transparency in presence of bFGF With regard to previous studies that demonstrated promotion of LE-dependent lens fiber and lentoid formation by bFGF, 2,10,11 Matrigel culture of aggregates in regular medium was compared with culture in regular medium supplemented with 100 ng/mL bFGF (Fig. 5).…”

Section: -15mentioning

confidence: 99%

“…6,[9][10][11][12] To generate lens-like structures with consistent morphologies with regard to size, transparency, and participating cell types, we tested whether MLE cells were capable of forming lens-like structures after aggregation and culture in Matrigel similar to newt IPE cells.…”

Section: Matrigel Promotes Lentoid Formation Of Mle Aggregatesmentioning

confidence: 99%

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Intrinsic Lens Forming Potential of Mouse Lens Epithelial versus Newt Iris Pigment Epithelial Cells in Three-Dimensional Culture

HoffmannAndrea

NakamuraKenta²,

TsonisPanagiotis³

2014

Tissue Engineering Part C: Methods

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Adult newts (Notophthalmus viridescens) are capable of complete lens regeneration that is mediated through dorsal iris pigment epithelial (IPE) cells transdifferentiation. In contrast, higher vertebrates such as mice demonstrate only limited lens regeneration in the presence of an intact lens capsule with remaining lens epithelial cells. To compare the intrinsic lens regeneration potential of newt IPE versus mouse lens epithelial cells (MLE), we have established a novel culture method that uses cell aggregation before culture in growth factor-reduced MatrigelÔ. Dorsal newt IPE aggregates demonstrated complete lens formation within 1 to 2 weeks of Matrigel culture without basic fibroblast growth factor (bFGF) supplementation, including the establishment of a peripheral cuboidal epithelial cell layer, and the appearance of central lens fibers that were positive for aAcrystallin. In contrast, the lens-forming potential of MLE cell aggregates cultured in Matrigel was incomplete and resulted in the formation of defined-size lentoids with partial optical transparency. While the peripheral cell layers of MLE aggregates were nucleated, cells in the center of aggregates demonstrated a nonapoptotic nuclear loss over a time period of 3 weeks that was representative of lens fiber formation. Matrigel culture supplementation with bFGF resulted in higher transparent bigger-size MLE aggregates that demonstrated increased appearance of bB1-crystallin expression. Our study demonstrates that bFGF is not required for induction of newt IPE aggregate-dependent lens formation in Matrigel, while the addition of bFGF seems to be beneficial for the formation of MLE aggregate-derived lens-like structures. In conclusion, the three-dimensional aggregate culture of IPE and MLE in Matrigel allows to a higher extent than older models the indepth study of the intrinsic lensforming potential and the corresponding identification of lentogenic factors.

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Section: Matrigel Promotes Lentoid Formation Of Mle Aggregatessupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Changes In Mle Aggregate Bb1-crystallin Expression In Presenmentioning

confidence: 99%

Section: -15mentioning

confidence: 99%

Section: Matrigel Promotes Lentoid Formation Of Mle Aggregatesmentioning

confidence: 99%

See 3 more Smart Citations

Intrinsic Lens Forming Potential of Mouse Lens Epithelial versus Newt Iris Pigment Epithelial Cells in Three-Dimensional Culture

HoffmannAndrea

NakamuraKenta²,

TsonisPanagiotis³

2014

Tissue Engineering Part C: Methods

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FGF receptor-1 (flg) expression is correlated with fibre differentiation during rat lens morphogenesis and growth

Iongh¹,

Lovicu²,

Hanneken³

et al. 1996

Dev. Dyn.

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Spatio‐temporal distribution of acidic and basic FGF indicates a role for FGF in rat lens morphogenesis

Iongh

McAvoy

1993

Developmental Dynamics

111

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As part of an investigation into the role FGF in lens development, we have studied the distribution of both aFGF and bFGF during eye morphogenesis from embryonic days 10 to 18 (E10–E18) in the rat. For aFGF, reactivity was found only in ectoderm at E10, prior to contact between the optic vesicle and presumptive lens ectoderm. During lens placode formation (E11) there was a transient, diffuse reactivity for aFGF in anterior optic vesicle cells directly apposed to the labelled ectoderm of the lens placode. At E12 the diffuse reactivity of the lens placode had changed to a discrete localisation along the basolateral surfaces of differentiating cells in the lens pit. Similar reactivity was associated with neuroblasts along the inner margin of the optic cup. At the early lens vesicle stage (E13) the baso‐lateral aFGF‐like reactivity associated with elongating lens cells was more intense and extensive. From the late lens vesicle stage (E14) to E18, reactivity in the lens was increasingly restricted to the equatorial regions which incorporate the germinative and transitional zones. From E16 to E18, aFGF‐like reactivity in the retina was predominantly localised in the peripheral regions corresponding to the developing ciliary body and iris and in the central retina associated with ganglion cell axons. For bFGF, weak reactivity was detectable as early as E13 in the developing lens capsule and increased in intensity during lens development with the posterior capsule reacting more intensely than the anterior capsule. Retinal bFGF‐like reactivity was first detected at E14, associated with differentiating ganglion cells in the central retina. From E16 to E18 the retinal ganglion cells showed increasing reactivity and the pattern of reactivity followed the centro‐peripheral pattern of retinal development. Thus reactivity for aFGF is first detected in presumptive lens ectoderm and subsequently in optic vesicle cells which are closely associated with lens ectoderm. This raises the possibility that aFGF may be involved in inductive interactions between presumptive lens ectoderm and optic vesicle. Furthermore the localisation patterns established for both aFGF and bFGF during lens and retina morphogenesis suggest an important role for FGF in regulating their morphogenesis and growth. © 1993 Wiley‐Liss, Inc.

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Induction of Lens Fibre Differentiation by Acidic and Basic Fibroblast Growth Factor (FGF)

Cited by 142 publications

References 33 publications

Intrinsic Lens Forming Potential of Mouse Lens Epithelial versus Newt Iris Pigment Epithelial Cells in Three-Dimensional Culture

Intrinsic Lens Forming Potential of Mouse Lens Epithelial versus Newt Iris Pigment Epithelial Cells in Three-Dimensional Culture

FGF receptor-1 (flg) expression is correlated with fibre differentiation during rat lens morphogenesis and growth

Spatio‐temporal distribution of acidic and basic FGF indicates a role for FGF in rat lens morphogenesis

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