Induction of lactoferrin gene expression by innate immune stimuli in mouse mammary epithelial HC-11 cells

Li, Yin; Limmon, Gino V.; Imani, Farhad; Teng, Christina T.

doi:10.1016/j.biochi.2008.04.014

Cited by 23 publications

(20 citation statements)

References 52 publications

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“…For instance lactoferrin expression is known to be induced during mastitis (e.g., [140]) and lactoferrin expression increases dramatically after treatment of mammary epithelial cells with bacteria lipopolysaccharides or double stranded RNA through PKC, NF-kappaB, and MAPK pathways [141]. Transcript abundance of LTF is also under control of a specific miRNA (miR-214) [142].…”

Section: Regulation Of Milk Protein Expressionmentioning

confidence: 99%

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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Section: Regulation Of Milk Protein Expressionmentioning

confidence: 99%

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Bionaz¹,

Hurley²,

Loor³

2012

Milk Protein

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“…Moreover, the Lf gene is regulated by innate immune stimuli and cRel/NF-jB binding sites have been identified in the promoter of the bovine Lf gene and proved to be highly responsive to LPS induction (Zheng et al 2005). Recently, it has been shown that Lf expression is induced in the bovine mammary gland in response to Staphylococcus aureus and Escherichia coli infection (Griesbeck-Zilch et al 2008) and in normal murine mammary gland epithelial cells by LPS, a major component of the outer membrane of gram negative bacteria, or dsRNA which mimics viral infection (Li et al 2009). Until now, no data are available concerning the putative presence of inflammatory response elements and their eventual functionality in the human Lf and DLf promoter regions.…”

Section: Introductionmentioning

confidence: 98%

Discrimination and evaluation of lactoferrin and delta-lactoferrin gene expression levels in cancer cells and under inflammatory stimuli using TaqMan real-time PCR

et al. 2010

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The lactoferrin gene is known to be expressed either constitutively or under inducible conditions such as hormonal stimuli or inflammation. Its transcription from alternative promoters leads to two products, lactoferrin (Lf) and delta-lactoferrin (DeltaLf) mRNAs the expressions of which are altered during oncogenesis. The comparison of the two enhancer/promoter regions revealed that the two isoforms might be differentially trans-activated. Nevertheless, concomitant expression of both transcripts has been found in some normal tissues and in a subset of breast cancer cell lines and biopsies. Moreover, we found putative inflammatory response elements in both P1 and P2 promoter regions suggesting that both Lf and DeltaLf might be upregulated under inflammatory stimuli. Therefore, a duplex Taqman gene expression assay has been developed and used to profile mRNA expression of the Lf gene in the case of cancer and under inflammatory conditions. Discrimination between the two transcripts is achieved by using a primer pairs/probe set within exon 1beta for DeltaLf and a primer pairs/probe set within exon 1 and exon 2 for Lf. In this study, we confirmed that Lf/DeltaLf Taqman gene expression assay is a powerful tool to investigate the expression of both Lf and DeltaLf transcripts. We also showed that lymphocytes and leukocytes isolated from fresh human blood expressed an extremely high level of DeltaLf messengers. An extensive series of cancer cell lines has been studied confirming that both P1 and P2 promoter regions of the Lf gene are downregulated or silenced in the case of cancer. Furthermore, using stimulation by bacterial lipopolysaccharides (LPS), we showed that in MDA-MB-231 and HT-29 epithelial cells, Lf expression is strongly increased with a higher expression level in MDA-MB-231 whereas DeltaLf expression is not. These results suggest that the NF-kappaB/cRel response elements present in the P1 promoter region are functional whereas those present in the P2 promoter region are not and show that DeltaLf is not regulated in inflammatory conditions.

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“…Primers designed by Li et al (2009) were used to detect and amplify the lactoferrin gene (forward primer 5'-AAACAAGCATCGGGATTCCAG-3'…”

Section: Real-time Pcrmentioning

confidence: 99%

Lactoferrin affects the adherence and invasion of Streptococcus dysgalactiae ssp. dysgalactiae in mammary epithelial cells

O’Halloran

Beecher

Chaurin

et al. 2016

Journal of Dairy Science

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Streptococcus dysgalactiae spp. dysgalactiae is an important causative agent of bovine mastitis worldwide. Lactoferrin is an innate immune protein that is associated with many functions including immunomodulatory, antiproliferative and antimicrobial properties. This study aimed to investigate the interactions between lactoferrin and a clinical bovine mastitis isolate, S. dysgalactiae spp. dysgalactiae DPC5345. Initially a deliberate in vivo bovine intramammary challenge was performed with S. dysgalactiae DPC5345. Results demonstrated a significant difference in lactoferrin mRNA levels in milk cells between the control and infused quarters 7 hours post infusion. Milk lactoferrin levels in the S. dysgalactiae DPC5345 infused quarters were significantly increased compared to control quarters at 48 h post infusion. In vitro studies demonstrated that lactoferrin had a bacteriostatic effect on the growth of S. dysgalactiae DPC5345 and significantly decreased the ability of the bacteria to internalise into HC-11 mammary epithelial cells. Confocal microscopy images of HC-11 cells exposed to S. dysgalactiae and lactoferrin further supported this effect by demonstrating reduced invasion of bacteria to HC-11 cells. The combined data suggests that a bovine immune response to S. dysgalactiae infection includes a significant increase in lactoferrin expression in vivo and, based on in vitro data, lactoferrin limits mammary cell invasion of this pathogen by binding to the bacteria and preventing its adherence.

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Induction of lactoferrin gene expression by innate immune stimuli in mouse mammary epithelial HC-11 cells

Cited by 23 publications

References 52 publications

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Milk Protein Synthesis in the Lactating Mammary Gland: Insights from Transcriptomics Analyses

Discrimination and evaluation of lactoferrin and delta-lactoferrin gene expression levels in cancer cells and under inflammatory stimuli using TaqMan real-time PCR

Lactoferrin affects the adherence and invasion of Streptococcus dysgalactiae ssp. dysgalactiae in mammary epithelial cells

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