Induction of immediate early genes and apoptosis after ischemia/reperfusion in fatty liver rats

Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury: a possible correlation with the proliferation or apoptosis of intestinal epithelial cells

“…Recently, immediate early genes are thought to play an important role in the process of inducing apoptosis or the regeneration of various cells, and indeed, transcriptional activation of AP-1 family encoded by immediate early genes was observed in the I/R injured neurons [12], myocardium [13], kidney [14], and liver [15][16][17][18]. In a rat intestinal I/R injury model, apoptosis and regeneration of the IECs were observed after reperfusion [19,20].…”

Effects of preconditioning on ischemia/reperfusion injury of hepatocytes determined by immediate early gene transcription

“…The induction of apoptosis may correlate with the severity of tissue ischemic damage. We have previously reported that enhanced and persistent expression of IEGs was associated with severe injury and poor survival in experimental animals subjected to hepatic I‐R, liver transplantation, or hemorrhagic shock 8,18,19. Thus, it is reasonable to assume that IPC may lead to a reduction in IEG expression, which, subsequently, results in significant reduction of apoptosis after I‐R.…”

“…Preparation of total RNA and Northern blot analysis were carried out as described previously 8,18,19. Total RNA was extracted from the frozen liver specimen with Isogen (Amersham, Sydney, Australia) and the liquid was size‐fractionated by 1% (w/v) agaroseformaldehyde gel electrophoresis, transferred onto Hybond‐N nylon membranes (Amersham) by capillary transfer, using 10 × SSC (1 × SSC = 0.15 M NaCl, and 0.015 M sodium citrate), and baked for 2 h and 80°C.…”

“…We have previously shown, in experimental models, that the expression of c‐fos and c‐jun increases in the liver after various insults, including liver ischemia, liver transplantation, and even hemorrhagic shock 8,18,19. In these models, increased expressions of IEGs was associated with severe hepatic damage, followed by liver regeneration.…”

“…[12][13][14][15][16][17] We have previously shown, in experimental models, that the expression of c-fos and c-jun increases in the liver after various insults, including liver ischemia, liver transplantation, and even hemorrhagic shock. 8,18,19 In these models, increased expressions of IEGs was associated with severe hepatic damage, followed by liver Abstract Ischemia reperfusion (I-R) of the liver induces various events leading to cell death (apoptosis) and subsequent cells proliferation. Recent experimental studies have described the protective effect of ischemic preconditioning (IPC) on I-R injury of the liver.…”