Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury: a possible correlation with the proliferation or apoptosis of intestinal epithelial cells

Shima, Yuichi; Tajiri, Tatsuro; Taguchi, Tomoaki; Suita, Sachiyo

doi:10.1016/j.jpedsurg.2005.12.025

Cited by 24 publications

(24 citation statements)

References 25 publications

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“…The data of histopathological assessments according to Park et al (1990)'s grading of the mucosal damage. This is in accordance with result of some investigators who reported non significant increase in the number of PCNA-positive cells in the section obtained after 240 min from reperfusion (Shima et al, 2006). Our study demonstrated that the expressing levels of PCNA after reperfusion were higher than the levels of control in both crypt and villus epithelial cells.…”

Section: Discussionsupporting

confidence: 93%

Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury

et al. 2015

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SUMMARY:The purpose of this study, ischemia reperfusion injury in rats, Potentilla fulgens is to investigate the protective effects. Wistar albino rats (n= 30) weighing 180-220 g were used in the experiment. Group 1 animals underwent sham laparotomy without ischemiareperfusion injury. Group 2 animals underwent laparotomy and occlusion of superior mesenteric arteries for 30 min followed by 20 min of reperfusion without pretreatment. The Potentilla fulgens group received 400 mg/kg/day Potentilla fulgens intraperitoneally 5 days before Ischemia-reperfusion injury. There was a significant difference between the group with ischemia-reperfusion group Potentilla fulgens (p<0.0001). In statistical analysis of the MDA level, data were obtained after a respective measurement in all groups. Potentilla fulgens group with ischemia-reperfusion group was a significant decrease in MDA (p<0.001). In the period after ischemia-reperfusion, marked PCNA immunoreactivities were observed in the nuclei of crypt and villus cell. In ischemia reperfusion group, the number of PCNA immunoreactivity is quite advanced and they extended throughout the middle part of the intestine folds. The number of TUNEL-positive nuclei were also developed. In ischemia-reperfusion plus P. fulgens group, the intestinal epithelium with only a few PCNA immunoreactive nuclei. TUNEL positive nuclei were noted in the gut lumen and mucosal close differentiated goblet cells. We showed that Potentilla fulgens extract significantly prevented mucosal lesions caused by intestinal ıschemia-reperfusion.

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Section: Discussionsupporting

confidence: 93%

Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury

et al. 2015

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“…Studies have shown that intestinal epithelial cell apoptosis is a significant contributor to mucosal barrier dysfunction. Immediate early genes, such as c- fos and c -jun are involved in the apoptosis of intestinal epithelial cells [22,23]. Some other factors, such as p38 MAPK, IL-6, HGF, and KGF, were also reported to be involved in the apoptosis of intestinal epithelial cells after I/R injury [24–27].…”

Section: Discussionmentioning

confidence: 99%

“…Inhibition of Notch signaling activation by γ-secretase inhibitors also lead to disturbed intestinal homeostasis [28–30]. The published studies have shown that the PCNA-positive cells increased at 1 to 6 h after reperfusion and TUNEL-positive cells increased later than PCNA-positive cells in a rat I/R model [22,23,31]. Our previous studies have investigated the role of Notch signaling in the proliferation of intestinal epithelial cells [19,32].…”

Section: Discussionmentioning

confidence: 99%

The Canonical Notch Signaling Was Involved in the Regulation of Intestinal Epithelial Cells Apoptosis after Intestinal Ischemia/Reperfusion Injury

Zhang¹,

Cheng²,

Xiao³

et al. 2014

IJMS

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Notch signaling plays a critical role in the maintenance of intestinal homeostasis. The aim of the present study was to investigate the role of Notch signaling in the apoptosis of intestinal epithelial cells after intestinal ischemia reperfusion (I/R) injury. Male C57BL/6 mice were subjected to sham operation or I/R injury. Intestinal tissue samples were collected at 12 h after reperfusion. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) staining showed that intestinal I/R injury induced significantly increased apoptosis of intestinal epithelial cells. Meanwhile, the mRNA expression of Jagged1, DLL1, Notch2, and Hes5, and protein expression of NICD2 and Hes5 were increased significantly after I/R injury in intestinal epithelial cells. In an in vitro IEC-6 culture model, flow cytometry analyses showed that inhibition of Notch signaling by γ-secretase inhibitor DAPT and the suppression of Hes5 expression using siRNA both significantly increased the apoptosis of IEC-6 cells under the condition of hypoxia/reoxygenation (H/R). In conclusion, the Notch2/Hes5 signaling pathway was activated and involved in the regulation of intestinal epithelial cells apoptosis in intestinal I/R injury.

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“…Many inflammatory responses, especially in gut, are mediated by activation of transcription factors such as AP-1 [13,14]. Gut ischemia/reperfusion (I/R) injury triggers AP-1 activation, and programmed cell death and subsequent cellular regeneration after I/R stress are related to expression patterns of some AP-1 proteins [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

c-Jun, Fra-2, and ATF-2 Immunoreactivity in the Jejunal Tissues of the Healthy Rat

Kekli̇koğlu

2008

Dig Dis Sci

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The aim of this study was to compare the localization of some activator protein-1 (AP-1) proteins in healthy rat jejunum. For this purpose, the AP-1 members c-Jun, Fra-2, and ATF-2 immunoreactivity (c-Jun-IR, Fra-2-IR, ATF-2-IR) in villus epithelial cells (ECs), intravillous lamina propria cells (LPCs), crypt cells (CCs), and smooth muscle cells (SMCs) were analyzed by immunohistochemical methods. Among all the cell groups, the lowest positivity ratio was found in c-Jun-IR and the highest positivity ratio was found in ATF-2-IR. For each group of ECs, LPCs, CCs, and SMCs, c-Jun-IR, Fra-2-IR, and ATF-2-IR were compared and statistically significant differences found. There were no significant differences among the cell groups with respect to c-Jun-IR and Fra-2-IR, but there was a statistically significant difference in ATF-2-IR. These findings suggest that each member of AP-1 is expressed differently and that ATF-2 is more active than c-Jun and Fra-2 in physiological conditions in healthy rat jejunum.

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Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury: a possible correlation with the proliferation or apoptosis of intestinal epithelial cells

Cited by 24 publications

References 25 publications

Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury

Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury

The Canonical Notch Signaling Was Involved in the Regulation of Intestinal Epithelial Cells Apoptosis after Intestinal Ischemia/Reperfusion Injury

c-Jun, Fra-2, and ATF-2 Immunoreactivity in the Jejunal Tissues of the Healthy Rat

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