Induction of Il-2 Receptor Expression in Vivo Response to Allogeneic Cells

Kroczek, Richard A.; Black, Christopher D. V.; Barbet, Jacques; Edison, Linette J.; Shevach, Ethan M.

doi:10.1097/00007890-198710000-00018

Cited by 14 publications

(12 citation statements)

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“…Treatment with anti-CD25 reduced this percentage to 4.6 Ϯ 1.0% ( p Ͻ 0.01). In addition to being expressed on the surfaces of Treg, CD25 is also transiently up-regulated on the surfaces of Teff after activation (35,36). To confirm that anti-CD25 treatment depleted Treg, we analyzed the proportion of diaphragm CD4 ϩ cells that expressed Foxp3 at 20 dpi.…”

Section: Depletion Of Naturally Occurring Treg During Muscle Infectionmentioning

confidence: 99%

Coordinated Control of Immunity to Muscle StageTrichinella spiralisby IL-10, Regulatory T Cells, and TGF-β

Beiting¹,

Gagliardo²,

Hesse

et al. 2007

The Journal of Immunology

129

135

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We previously demonstrated that IL-10 is critical in the control of acute inflammation during development of Trichinella spiralis in the muscle. In this study, we use gene-targeted knockout mice, adoptive transfer of specific T cell populations, and in vivo Ab treatments to determine the mechanisms by which inflammation is controlled and effector T cell responses are moderated during muscle infection. We report that CD4+CD25− effector T cells, rather than CD4+CD25+ regulatory T cells, suppress inflammation by an IL-10-dependent mechanism that limits IFN-γ production and local inducible NO synthase induction. Conversely, we show that depletion of regulatory T cells during infection results in exaggerated Th2 responses. Finally, we provide evidence that, in the absence of IL-10, TGF-β participates in control of local inflammation in infected muscle and promotes parasite survival.

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Section: Depletion Of Naturally Occurring Treg During Muscle Infectionmentioning

confidence: 99%

Coordinated Control of Immunity to Muscle StageTrichinella spiralisby IL-10, Regulatory T Cells, and TGF-β

Beiting¹,

Gagliardo²,

Hesse

et al. 2007

The Journal of Immunology

129

135

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“…NaTregs constitutively express CD25 (9,12), which is widely used to define these cells in naive and also inflammatory settings, although activated effector T cells (7,8) and perhaps certain inTregs also express CD25 (10). Recently, it was discovered that activated/memory subsets of naTregs express the integrin ␣ E (CD103)␤ 7 (21)(22)(23).…”

Section: In Secondary Lymphoid Organs Of Infected and Noninfected Recmentioning

confidence: 99%

“…In two separate experiments, cell isolates from liver, MLN, and spleen of infected mice (7,9, and 12 wk postinfection) and naive controls were stained with specific Abs for CD4, CD25, and CD103 and then analyzed by flow cytometry. Conformity of the infections was verified by individual worm and egg counts (data not shown).…”

Section: Egg-induced Inflammation Causes a Modest Increase In The Frementioning

confidence: 99%

“…An important regulatory role for CD4 ϩ CD25 ϩ T cells also was demonstrated in a model of filariasis, another chronic helminth infection (6). However, because CD25, the ␣-chain of the IL-2 receptor, also is expressed by activated effector T cells (7,8) and different populations of Tregs (9,10), the identity of the Treg population in schistosomiasis could not be determined by these experiments.…”

mentioning

confidence: 93%

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Naturally Occurring CD4+Foxp3+ Regulatory T Cells Are an Essential, IL-10-Independent Part of the Immunoregulatory Network inSchistosoma mansoniEgg-Induced Inflammation

Baumgart

Tompkins

Leng

et al. 2006

The Journal of Immunology

129

125

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In acute and chronic schistosomiasis, survival of the host requires a carefully balanced immune response against highly immunogenic parasite eggs. We characterized the phenotype, distribution, and functional role of CD4+Foxp3+ naturally occurring regulatory T cells (naTregs) in schistosome egg-induced inflammation. In adoptive transfer experiments and by intracellular staining for Foxp3, we demonstrate significant frequencies of naTregs in hepatic granulomas and draining lymphoid tissues of mice infected with the trematode Schistosoma mansoni. Strikingly, egg-induced inflammation does not change the normal ratio between naTregs and effector CD4+ T cells at the inflammatory site or in lymphoid organs in acute or chronic disease. However, increasing frequencies of CD103-expressing cells in the naTreg compartment indicate a change in phenotype for naTregs with disease progression. Because CD103 was described recently as an activation marker for naTregs, we speculate that naTregs in chronic schistosomiasis are potentially more suppressive. Furthermore, we found that most naTregs do not contribute to egg-induced IL-4 and IL-10 production. Importantly, depletion of CD25+ naTregs strongly enhances the frequency of IL-4-producing effector T cells in acute egg-induced inflammation. It does not change clonal expansion of activated CD4+ T cells. This regulation of egg-induced cytokine production does not require the presence of IL-10. These data demonstrate that naTregs limit egg-induced effector-cytokine production in our model. Our results identify naTregs as an important, IL-10-independent part of the regulatory network in schistosome egg-induced inflammation.

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“…Although injection of 3H-TdR in vivo may be the best approach to estimate the spontaneous rate of DNA synthesis in LN [8], we used the alternative method of Kroczek et al [3,9], LN cells were first col lected and 3H-TdR incorporation was determined during subsequent in vitro culture, cpm significantly (p < 0.01 ) increased at 10 days after transplantation of FI or F/D granulomas ( fig. 3a), indicating that prolif eration of LN cells occurred as the LN cell numbers increased at this time.…”

Section: Spontaneous and Pha-stimulated 3 H-tdr Incorporationmentioning

confidence: 99%

Immunosuppression and in vivo T Cell Function in Granulomatous Inflammation

Fujioka

Pincelli

Hashimoto

et al. 1989

Int Arch Allergy Immunol

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Subcutaneous grafts of fresh or freeze-dried hepatic schistosome egg granulomas in inbred mice caused increased numbers of total cells, T cell subsets, and elevated DNA synthesis in regional lymph nodes. This in vivo T cell activation was not apparent by in vitro tests. Spleen cells were unaffected both in vivo and in vitro. Immunosuppressive therapy with ciclosporin or monoclonal antibodies to T cell subsets profoundly depressed the T cell numbers and function, even when primed by a prior graft. Subsequent grafting partly reversed these effects, and T cells became activated. The findings indicate that in vitro assessment of activation and suppression of T cell function does not necessarily correspond to the events occurring in vivo.

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Induction of Il-2 Receptor Expression in Vivo Response to Allogeneic Cells

Cited by 14 publications

References 0 publications

Coordinated Control of Immunity to Muscle StageTrichinella spiralisby IL-10, Regulatory T Cells, and TGF-β

Coordinated Control of Immunity to Muscle StageTrichinella spiralisby IL-10, Regulatory T Cells, and TGF-β

Naturally Occurring CD4+Foxp3+ Regulatory T Cells Are an Essential, IL-10-Independent Part of the Immunoregulatory Network inSchistosoma mansoniEgg-Induced Inflammation

Immunosuppression and in vivo T Cell Function in Granulomatous Inflammation

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