Induction of IFN-γ by a highly branched 1,3-β-d-glucan from Aureobasidium pullulans in mouse-derived splenocytes via dectin-1-independent pathways

Tada, Rui; Yoshikawa, Masashi; Ikeda, Futoshi; Adachi, Yoshiyuki; Kato, Yuichi; Kuge, Takao; Tanioka, Asuka; Ishibashi, Keiichiro; Tsubaki, Kazufumi; Ohno, Naohito

doi:10.1016/j.bbrc.2010.12.125

Cited by 18 publications

(13 citation statements)

References 36 publications

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“…Several molecules have been suggested as β‐glucan receptors, such as complement receptor type 3 (CR3), and dectin‐1 . Although the AP‐FBG receptor of PPDC has not been elucidated , we hypothesize that IL‐6 secretion was induced by stimulation of PPDCs by AP‐FBG via some kind of receptor.…”

Section: Discussionmentioning

confidence: 93%

Enhancement of Intestinal Immune Function in Mice by β‐D‐Glucan from Aureobasidium Pullulans ADK‐34

et al. 2013

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b-Glucans, glucose polymers that are the main constituents of the outer cell walls of micro-organisms such as fungi and yeast, are known to play an immunostimulatory role. We prepared b-glucan (b-(1-3),(1-6)-D-glucan) from an edible cultured fungus through fermentation techniques using a strain of Aureobasidium pullulans ADK-34. The purity of this b-glucan preparation (AP-FBG) was demonstrated to be high through various instrumental analyses. We then examined the effects of AP-FBG on intestinal immune systems. We prepared Peyer's patch (PP) cells and measured interleukin (IL)-5, IL-6, and IgA production in culture media with AP-FBG. We found that both cytokines and IgA increased; furthermore, IL-6 secreted by PP dendritic cells (PPDCs) cultured in the presence of AP-FBG significantly increased. We tested IgA production after oral administration of AP-FBG for 2 weeks and found that AP-FBG tended to promote the production of IgA in the small intestine. Interestingly, we observed a significant increase in IgA production in the small intestines of mice treated with cyclophosphamide (CY; an immunosuppressant) after oral administration of AP-FBG diet compared with CY-treated and control diet mice. Production of IL-6 and IgA by PP cells and IL-6 production by PPDCs in AP-FBG-fed and CY-treated mice also increased. These results demonstrate that AP-FBG has the ability to activate PPDC and induce IL-6 production and IgA secretion in PP cells. These abilities were more clearly expressed when AP-FBG was orally administered in a CY-induced immunosuppressed condition. Therefore, AP-FBG may be a useful ingredient for preparing functional foods with immunomodulatory activities.

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Section: Discussionmentioning

confidence: 93%

Enhancement of Intestinal Immune Function in Mice by β‐D‐Glucan from Aureobasidium Pullulans ADK‐34

et al. 2013

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“…Curdlan is a particulate β-glucan [ 35 , 36 ], whereas AP-PG is water soluble [ 8 , 9 ]. Futher, it has been reported that AP-PG does not exhibit binding activity to dectin-1 [ 37 ], while curdlan is known to be an agonist for dectin-1 and is recognized by dectin-1 [ 38 , 39 ]. The results of our preliminary experiments using antagonistic antibodies against CR3 and dectin-1 showed that the TRAIL induction after stimulation with AP-PG is mainly dependent on CR3.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of Macrophages with the β-Glucan Produced by Aureobasidium pullulans Promotes the Secretion of Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)

et al. 2015

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A β-glucan produced by Aureobasidium pullulans (AP-PG) is consisting of a β-(1,3)-linked main chain with β-(1,6)-linked glucose side residues. Various β-glucans consisting of β-(1,3)-linked main chain including AP-PG are believed to exhibit anti-tumor activities, and actually, anti-tumor activities of AP-PG in mice have been demonstrated. In this study, we demonstrate that stimulation with AP-PG induces TRAIL expression in mouse and human macrophage-like cell lines. TRAIL is known to be a cytokine which specifically induces apoptosis in transformed cells, but not in untransformed cells. The expression of TRAIL mRNA after stimulation with AP-PG was increased in RAW264.7 cells, Mono Mac 6 cells, and macrophage-differentiated THP-1 cells. The mRNA expression of TNF-α and FasL is only weakly increased after stimulation with AP-PG. The induction activity of TRAIL by curdlan, a bacterial β-glucan, was very similar to that by AP-PG in RAW264.7 cells, but weaker in macrophage-differentiated THP-1 cells. Activation of caspases was found in HeLa cells after treatment with the supernatant of cultured medium from AP-PG-stimulated Mono Mac 6 cells, and was inhibited by the anti-TRAIL neutralizing antibody. These findings suggest that the stimulation with AP-PG effectively induces TRAIL in macrophages, and that it may be related to apoptosis induction of tumor cells.

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“…Macrophages are important to activate the immune system in response to stimulation of β-glucans consisting of β-(1,3)-linked main chains, and CR3 (complement receptor 3) and dectin-1 (dendritic cell-associated C-type lectin-1) are known to be major receptors for recognition of these β-glucans in macrophages29. It has been reported that AP-PG does not exhibit binding activity to dectin-130, while curdlan is known to be an agonist for dectin-1 and is recognized by dectin-13132. This difference in dectin-1 binding affinities between curdlan and AP-PG may be related in the differences in the induction efficiency of ISGs.…”

Section: Discussionmentioning

confidence: 99%

“…The A. pullulans -produced β-glucan (AP-PG) consists of a β-1,3-D-glycosidic linked main chain and β-1,6-D-glycosidic linked side chains, and has an immune modulating activity3. A. pullulans -cultured fluid containing AP-PG as a main compound is thought to induce beneficial effects on health, and is marketed as a food supplement.…”

mentioning

confidence: 99%

Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines

Muramatsu¹,

Kawata

Aoki³

et al. 2014

Sci Rep

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A β-(1,3),(1,6)-D-glucan produced by A. pullulans (AP-PG) is known to be an immune stimulating agent. In this study, we demonstrate that the stimulation with AP-PG effectively induces the interferon (IFN) stimulated genes (ISGs) in macrophage-like cell lines. The ISGs, Mx1, ISG15, and viperin mRNAs were significantly increased in RAW264.7 cells after stimulation with AP-PG. The stimulation with AP-PG transiently induced IFN-β mRNA. However, the expression of viperin mRNA was also increased after stimulation with AP-PG even when new protein synthesis was completely blocked by treatment with cycloheximide. Further, in IFN-α receptor knockdown RAW264.7 cells, AP-PG stimulation more effectively induced viperin mRNA compared with that of IFN-α stimulation. The phosphorylation of Ser 727 in STAT1 involved in the enhancement of STAT1 activation was immediately increased after stimulation with AP-PG. In addition, viperin mRNA expression induced after stimulation with IFN-α was significantly increased by combined stimulation with AP-PG. These results suggest that stimulation with AP-PG effectively induces the ISGs through the induction of IFN and the enhancement of STAT1-mediated transcriptional activation.

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Induction of IFN-γ by a highly branched 1,3-β-d-glucan from Aureobasidium pullulans in mouse-derived splenocytes via dectin-1-independent pathways

Cited by 18 publications

References 36 publications

Enhancement of Intestinal Immune Function in Mice by β‐D‐Glucan from Aureobasidium Pullulans ADK‐34

Enhancement of Intestinal Immune Function in Mice by β‐D‐Glucan from Aureobasidium Pullulans ADK‐34

Stimulation of Macrophages with the β-Glucan Produced by Aureobasidium pullulans Promotes the Secretion of Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)

Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines

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