Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines

Muramatsu, Daisuke; Kawata, Keiichiro; Aoki, Shigenobu; Uchiyama, Hirofumi; Okabe, Mitsuyasu; Miyazaki, Tatsuya; Kida, Hiroshi; Iwai, Atsushi

doi:10.1038/srep04777

Cited by 15 publications

(12 citation statements)

References 38 publications

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“…These pathways consist of the activation of transcription factors, such as nuclear factor kappa-light-chain-enhancer (NF-κB) of activated B cells and interferon regulatory factors (IRFs), which results in production of type I IFNs and pro-inflammatory cytokines. For instance, a type I IFN gene signature was promoted in human DCs that were stimulated with WGP and curdlan [69] as well as in human and murine macrophage cell lines stimulated with a β-glucan derived from A. pullulan [70]. More specifically, the intracellular receptors NOD1 and NOD2 can interact with the inflammasome NLRP3, which then leads to production of IL-1 cytokines [71].…”

Section: β-Glucan Receptorsmentioning

confidence: 99%

Consumption of β-glucans to spice up T cell treatment of tumors: a review

Graaff

Govers

Wichers

et al. 2018

Expert Opinion on Biological Therapy

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Adoptive T-cell treatments of solid cancers have evolved into a robust therapy with objective response rates surpassing those of standardized treatments. Unfortunately, only a limited fraction of patients shows durable responses, which is considered to be due to a T cell-suppressive tumor microenvironment (TME). Here we argue that naturally occurring β-glucans can enable reversion of such T cell suppression by engaging innate immune cells and enhancing numbers and function of lymphocyte effectors. Areas covered: This review summarizes timely reports with respect to absorption, trafficking and immune stimulatory effects of β-glucans, particularly in relation to innate immune cells. Furthermore, we list effects toward well-being and immune functions in healthy subjects as well as cancer patients treated with orally administered β-glucans, extended with effects of β-glucan treatments in mouse cancer models. Expert opinion: Beta-glucans, when present in food and following uptake in the proximal gut, stimulate immune cells present in gut-associated lymphoid tissue and initiate highly conserved pro-inflammatory pathways. When tested in mouse cancer models, β-glucans result in better control of tumor growth and shift the TME toward a T cell-sensitive environment. Along these lines, we advocate that intake of β-glucans provides an accessible and immune-potentiating adjuvant when combined with adoptive T-cell treatments of cancer.

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Section: β-Glucan Receptorsmentioning

confidence: 99%

Consumption of β-glucans to spice up T cell treatment of tumors: a review

Graaff

Govers

Wichers

et al. 2018

Expert Opinion on Biological Therapy

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“…The A. pullulans incubation fluid containing β-glucan as the main ingredient is consumed as a supplementary food in many countries as it represents the activation of the immune-stimulation. Also, A. pullulans incubation fluid is regarded as useful and effective for delaying the outbreaks of many diseases, and has been reported to show the activation of anti-tumor (Kimura et al, 2006), anti-allergy (Kimura et al, 2007), and anti-inflammation (Muramatsu et al, 2014) in the mouse model.…”

Section: Introductionmentioning

confidence: 99%

Effects of β-Glucan on the Release of Nitric Oxide by Macrophages Stimulated with Lipopolysaccharide

Choi

Lee

Hyeon

et al. 2016

Asian Australas. J. Anim. Sci

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This research analyzed the effect of β-glucan that is expected to alleviate the production of the inflammatory mediator in macrophagocytes, which are processed by the lipopolysaccharide (LPS) of Escherichia. The incubated layer was used for a nitric oxide (NO) analysis. The DNA-binding activation of the small unit of nuclear factor-κB was measured using the enzyme-linked immunosorbent assay-based kit. In the RAW264.7 cells that were vitalized by Escherichia coli (E. coli) LPS, the β-glucan inhibited both the combatant and rendering phases of the inducible NO synthase (iNOS)-derived NO. β-Glucan increased the expression of the heme oxygenase-1 (HO-1) in the cells that were stimulated by E. coli LPS, and the HO-1 activation was inhibited by the tin protoporphyrin IX (SnPP). This shows that the NO production induced by LPS is related to the inhibition effect of β-glucan. The phosphorylation of c-Jun N-terminal kinases (JNK) and the p38 induced by the LPS were not influenced by the β-glucan, and the inhibitory κB-α (IκB-α) decomposition was not influenced either. Instead, β-glucan remarkably inhibited the phosphorylation of the signal transducer and activator of transcription-1 (STAT1) that was induced by the E. coli LPS. Overall, the β-glucan inhibited the production of NO in macrophagocytes that was vitalized by the E .coli LPS through the HO-1 induction and the STAT1 pathways inhibition in this research. As the host immune response control by β-glucan weakens the progress of the inflammatory disease, β-glucan can be used as an effective immunomodulator.

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“…As noted above, the antiviral effects of APβG may also be realized through the expression of interferon-stimulated genes (ISGs) in macrophage-like cell lines. These findings suggest that APβG stimulation effectively promotes the expression of ISGs through inducing IFNs and enhancing STAT1-mediated transcriptional activity [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Biological Activity of High-Purity β-1,3-1,6-Glucan Derived from the Black Yeast Aureobasidium pullulans: A Literature Review

Suzuki¹,

Kusano

Kondo

et al. 2021

Nutrients

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The black yeast Aureobasidium pullulans produces abundant soluble β-1,3-1,6-glucan—a functional food ingredient with known health benefits. For use as a food material, soluble β-1,3-1,6-glucan is produced via fermentation using sucrose as the carbon source. Various functionalities of β-1,3-1,6-glucan have been reported, including its immunomodulatory effect, particularly in the intestine. It also exhibits antitumor and antimetastatic effects, alleviates influenza and food allergies, and relieves stress. Moreover, it reduces the risk of lifestyle-related diseases by protecting the intestinal mucosa, reducing fat, lowering postprandial blood glucose, promoting bone health, and healing gastric ulcers. Furthermore, it induces heat shock protein 70. Clinical studies have reported the antiallergic and triglyceride-reducing effects of β-1,3-1,6-glucan, which are indicators of improvement in lifestyle-related diseases. The primary and higher-order structures of β-1,3-1,6-glucan have been elucidated. Specifically, it comprises a single highly-branched glucose residue with the β-1,6 bond (70% or more) on a backbone of glucose with 1,3-β bonds. β-Glucan shows a triple helical structure, and studies on its use as a drug delivery system have been actively conducted. β-Glucan in combination with anti-inflammatory substances or fullerenes can be used to target macrophages. Based on its health functionality, β-1,3-1,6-glucan is an interesting material as both food and medicine.

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Stimulation with the Aureobasidium pullulans-produced β-glucan effectively induces interferon stimulated genes in macrophage-like cell lines

Cited by 15 publications

References 38 publications

Consumption of β-glucans to spice up T cell treatment of tumors: a review

Consumption of β-glucans to spice up T cell treatment of tumors: a review

Effects of β-Glucan on the Release of Nitric Oxide by Macrophages Stimulated with Lipopolysaccharide

Biological Activity of High-Purity β-1,3-1,6-Glucan Derived from the Black Yeast Aureobasidium pullulans: A Literature Review

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