Induction of Hypersensitivity Reactions to &lt;i&gt;Lactobacillus fermentum&lt;/i&gt; and Lipoteichoic Acid in Rabbits

Jackson, Dianne E.; Howlett, C. R.; Wicken, A. J.; Jackson, G. D. F.

doi:10.1159/000232770

Cited by 7 publications

(3 citation statements)

References 7 publications

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“…LTA is known to bind to epithelial cells (4,26), erythrocytes (7,46), phagocytes (25), and human platelets (5). Hypersensitivity reactions and suppression of the immune response in laboratory animals have been demonstrated to be affected by LTA (14,23). LTA also acts as a nonspecific T-lymphocyte mitogen (6).…”

Section: Resultsmentioning

confidence: 99%

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Miörner¹,

Johansson²,

Kronvall³

1983

Infect Immun

106

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MATERIALS AND METHODS Bacterial strains. A total of 56 bacterial strains were included in the study. The bacterial species and number of strains were as follows: group A streptococci, 34; group C streptococci, 5; group G streptococci, 6; Klebsiella pneumoniae, 3; Pseudomonas aeruginosa, 4; and Escherichia coli, 4. The strains were kindly provided by J. Rotta, Prague, Czechoslovakia (28 strains), and L. W. Wannamaker, Minneapolis, Minn.(2 strains), or were obtained consecutively from clinical specimens sent to the Clinical Microbiology Laboratory, University Hospital, Lund, Sweden. Strains were stored at -90°C in Todd-Hewitt broth supplemented with fetal calf serum and on blood agar plates at 4°C. Bacterial strains were inoculated in Todd-Hewitt broth and incubated for 15 to 17.5 h at 37°C. Bacteria were harvested by centrifugation at 3,500 rpm for 10 min and washed twice in phosphate-buffered 336 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Resultsmentioning

confidence: 99%

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Miörner¹,

Johansson²,

Kronvall³

1983

Infect Immun

106

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“…Fol lowing a second injection of the highest dose of L. jermentum (4 X 1010 cells), all rabbits developed fatal reactions, and these responses were also observed in the majori ty of rabbits given 3 or 4 injections of lower doses of L. jermentum cells [13]. No such phenomena were observed in mice following any regimen of L. jermentum cell injections.…”

Section: Discussionmentioning

confidence: 93%

Immune Responses to Lipoteichoic Acid: Comparison of Antibody Responses in Rabbits and Mice

Jackson¹,

Wicken²,

Jackson³

1981

Int Arch Allergy Immunol

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Humoral immune responses to lipoteichoic acid (LTA) as a surface antigen of Lactobacillus jermentum were assessed in rabbits and mice. Intravenous injection of rabbits with whole bacteria was effective, over a wide range of doses (10⁴ to 10¹⁰ cells), in eliciting antibodies to LTA. In mice, significant levels of anti-LTA antibodies were induced only following intraperitoneal injection of 10⁸ to 10⁹L. fermentum cells. Rabbit antibodies to LTA were predominantly of the IgM class and were specific for the polyglycerol phosphate and carbohydrate moieties of the LTA. In contrast, both IgM and IgG anti-LTA antibodies were produced in the mouse, and antibody specificity was restricted to the polyglycerol phosphate sequence.

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“…However, the class of specific antibodies during classical primary and subsequent re sponses to LTA has not been previously de scribed. In this and the accompanying pa pers [3,4] we report on several aspects of humoral immune responses to LTA as a bacterial cell surface antigen. The present report describes the primary and anamnestic response to LTA in rabbits, and so extends the immunobiological properties of LTA to include induction of IgM immunological memory in this species.…”

Section: Introductionmentioning

confidence: 97%

Induction of IgM Immunological Memory to Lipoteichoic Acids in Rabbits

Jackson¹,

Jackson²,

Wicken³

1981

Int Arch Allergy Immunol

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Intravenous immunisation of rabbits with 10⁹Lactobacillus fermentum cells elicited a response, specific for lipoteichoic acid (LTA), detectable as IgM plaque-forming cells (PFC) in the spleen by day 2 and as thiol-sensitive 19s antibodies in sera by day 3. Direct PFC responses peaked at day 6, with no indirect PFC demonstrable at that time. Specific IgG PFC appeared after 14 days. A second intravenous injection 5 weeks later induced a 10-fold higher IgM PFC response to LTA which reached a maximum on day 4. An enhanced specific IgG PFC response was also observed. Serum analysis showed further evidence of the anamnestic IgM response to LTA. The results are discussed in terms of the T-dependence of the LTA immunogen.

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Induction of Hypersensitivity Reactions to <i>Lactobacillus fermentum</i> and Lipoteichoic Acid in Rabbits

Cited by 7 publications

References 7 publications

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci

Immune Responses to Lipoteichoic Acid: Comparison of Antibody Responses in Rabbits and Mice

Induction of IgM Immunological Memory to Lipoteichoic Acids in Rabbits

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