Immune Responses to Lipoteichoic Acid: Comparison of Antibody Responses in Rabbits and Mice

Jackson, Dianne E.; Wicken, A. J.; Jackson, G. D. F.

doi:10.1159/000232757

Cited by 4 publications

(6 citation statements)

References 8 publications

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“…The litres of anti-LTA antibodies in the sera of animals which showed mild or moderate clinical signs were not significantly different from those in the groups which developed severe or fatal shock. In each of the dosage groups both thiol-sensitive and thiol-resis tant (shown in previous studies to be IgM and IgG, respectively) [5,10] antibodies to LTA were present. There was no significant correlation between the levels of anti-LTA activity present at the time of the i.v.…”

Section: Induction Of the Hypersensitive Statementioning

confidence: 80%

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Induction of Hypersensitivity Reactions to <i>Lactobacillus fermentum</i> and Lipoteichoic Acid in Rabbits

Jackson¹,

Howlett²,

Wicken³

et al. 1981

Int Arch Allergy Immunol

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Regimens of intravenous injections of saline-washed Lactobacillus fermentum elicited hypersensitivity reactions in rabbits. Pathological investigation revealed evidence consistent with induction of aggregate anaphylaxis, characterised by acute cor pulmonale. Additional evidence of similar tissue injury was observed in livers of rabbits which had received several intravenous injections of L. fermentum. Deposition of immune complexes in kidney glomeruli was demonstrated in only 1 out of 11 animals. Skin testing experiments revealed that lipoteichoic acid was involved in type I and type III antibody-mediated hypersensitive states. The involvement of bacterial cell surface components and extracellular products in such reactions implies a potential role in host tissue injury.

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Section: Induction Of the Hypersensitive Statementioning

confidence: 80%

“…Studies in this laboratory [5] have shown intravenous (i.v.) injections of Lactobacillus fermentum into rabbits give rise to substan tial levels of IgM and lgG antibodies specif ic for LTA.…”

Section: Introductionmentioning

confidence: 96%

Induction of Hypersensitivity Reactions to <i>Lactobacillus fermentum</i> and Lipoteichoic Acid in Rabbits

Jackson¹,

Howlett²,

Wicken³

et al. 1981

Int Arch Allergy Immunol

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“…The structural features of the LTA molecule suggest similarities to many of the T-inde pendent antigens and it may be that LTA easily triggers B cells to specific IgM secre tion. Indeed, intensive courses of L. fermen tum cell injections have induced only IgM antibodies to LTA in some rabbits [3].…”

Section: Discussionmentioning

confidence: 99%

“…However, the class of specific antibodies during classical primary and subsequent re sponses to LTA has not been previously de scribed. In this and the accompanying pa pers [3,4] we report on several aspects of humoral immune responses to LTA as a bacterial cell surface antigen. The present report describes the primary and anamnestic response to LTA in rabbits, and so extends the immunobiological properties of LTA to include induction of IgM immunological memory in this species.…”

Section: Introductionmentioning

confidence: 98%

Induction of IgM Immunological Memory to Lipoteichoic Acids in Rabbits

Jackson¹,

Jackson²,

Wicken³

1981

Int Arch Allergy Immunol

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Intravenous immunisation of rabbits with 10⁹Lactobacillus fermentum cells elicited a response, specific for lipoteichoic acid (LTA), detectable as IgM plaque-forming cells (PFC) in the spleen by day 2 and as thiol-sensitive 19s antibodies in sera by day 3. Direct PFC responses peaked at day 6, with no indirect PFC demonstrable at that time. Specific IgG PFC appeared after 14 days. A second intravenous injection 5 weeks later induced a 10-fold higher IgM PFC response to LTA which reached a maximum on day 4. An enhanced specific IgG PFC response was also observed. Serum analysis showed further evidence of the anamnestic IgM response to LTA. The results are discussed in terms of the T-dependence of the LTA immunogen.

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“…Lipoteichoic acid (LTA) is an obligatory component of the membrane of Gram-positive bacteria, including staphylococci (10,11), and it is capable of eliciting specific antibodies (Ab) (10,(12)(13)(14). In this regard, immunization of mice with purified native LTA in adjuvant elicited an anti-LTA antibody response that inhibited adherence of Streptococcus pyogenes to pharyngeal epithelial cells (15).…”

mentioning

confidence: 99%

Novel Synthetic (Poly)Glycerolphosphate-Based Antistaphylococcal Conjugate Vaccine

et al. 2013

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g Staphylococcal infections are a major source of global morbidity and mortality. Currently there exists no antistaphylococcal vaccine in clinical use. Previous animal studies suggested a possible role for purified lipoteichoic acid as a vaccine target for eliciting protective IgG to several Gram-positive pathogens. Since the highly conserved (poly)glycerolphosphate backbone of lipoteichoic acid is a major antigenic target of the humoral immune system during staphylococcal infections, we developed a synthetic method for producing glycerol phosphoramidites to create a covalent 10-mer of (poly)glycerolphosphate for potential use in a conjugate vaccine. We initially demonstrated that intact Staphylococcus aureus elicits murine CD4؉ T cell-dependent (poly)glycerolphosphate-specific IgM and IgG responses in vivo. Naive mice immunized with a covalent conjugate of (poly)glycerolphosphate and tetanus toxoid in alum plus CpG-oligodeoxynucleotides produced high secondary titers of serum (poly)glycerolphosphate-specific IgG. Sera from immunized mice enhanced opsonophagocytic killing of live Staphylococcus aureus in vitro. Mice actively immunized with the (poly)glycerolphosphate conjugate vaccine showed rapid clearance of staphylococcal bacteremia in vivo relative to mice similarly immunized with an irrelevant conjugate vaccine. In contrast to purified, natural lipoteichoic acid, the (poly)glycerolphosphate conjugate vaccine itself exhibited no detectable inflammatory activity. These data suggest that a synthetic (poly)glycerolphosphate-based conjugate vaccine will contribute to active protection against extracellular Gram-positive pathogens expressing this highly conserved backbone structure in their membrane-associated lipoteichoic acid.

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Immune Responses to Lipoteichoic Acid: Comparison of Antibody Responses in Rabbits and Mice

Cited by 4 publications

References 8 publications

Induction of Hypersensitivity Reactions to <i>Lactobacillus fermentum</i> and Lipoteichoic Acid in Rabbits

Induction of Hypersensitivity Reactions to <i>Lactobacillus fermentum</i> and Lipoteichoic Acid in Rabbits

Induction of IgM Immunological Memory to Lipoteichoic Acids in Rabbits

Novel Synthetic (Poly)Glycerolphosphate-Based Antistaphylococcal Conjugate Vaccine

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