Induction of Heteroallelic Reversions and Lethality in Saccharomyces cerevisiae Exposed to Radiations of Various LET ( 60 Co g Rays, Heavy Ions, and π - Mesons) in Air and Nitrogen Atmospheres

Raju, M.R.; Gnanapurani, M.; Stackler, B.; Martins, B. I.; Madhvanath, U.; Howard, J.; Lyman, John; Mortimer, Robert

doi:10.2307/3573356

Cited by 27 publications

(3 citation statements)

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“…During GC, DNA sequence from a donor chromosome is transferred to a homologous acceptor region between sister chromosomes or chromatids. GC is evolutionarily important, especially for DNA repair during recombination 32 33 34 . The CO-associated conversion tracts (COCTs) were reported to be <1-kb long in Arabodopsis 35 and 2 kb in yeast 10 .…”

Section: Resultsmentioning

confidence: 99%

Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize

2015

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Meiotic recombination drives eukaryotic sexual reproduction and the generation of genome diversity. Tetrad analysis, which examines the four chromatids resulting from a single meiosis, is an ideal method to study the mechanisms of homologous recombination. Here we develop a method to isolate the four microspores from a single tetrad in maize for the purpose of whole-genome sequencing. A high-resolution recombination map reveals that crossovers are unevenly distributed across the genome and are more likely to occur in the genic than intergenic regions, especially common in the 5′- and 3′-end regions of annotated genes. The direct detection of genomic exchanges suggests that conversions likely occur in most crossover tracts. Negative crossover interference and weak chromatid interference are observed at the population level. Overall, our findings further our understanding of meiotic recombination with implications for both basic and applied research.

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Section: Resultsmentioning

confidence: 99%

Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize

2015

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“…(1 974), we measured the rate of crossing over induced by irradiation with X rays or fission neutrons. If induced rates/anlage cell/Gy of crossover in the fly are quantitatively close to those of crossover in yeast (NAKAI and MORTIMER 1969;RAJU et al 1971), such agreement suggests that our knowledge of the mechanism of mitotic recombination in yeast may be applicable to that in the fly.…”

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confidence: 61%

Induced rates of mitotic crossing over and possible mitotic gene conversion per wing anlage cell in Drosophila melanogaster by X rays and fission neutrons.

Ayaki¹,

Fujikawa²,

Ryo³

et al. 1990

Genetics

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As a model for chromosome aberrations, radiation-induced mitotic recombination of mwh and flr genes in Drosophila melanogaster strain (mwh +/+ flr) was quantitatively studied. Fission neutrons were five to six times more effective than X rays per unit dose in producing either crossover-mwh/flr twins and mwh singles-or flr singles, indicating that common processes are involved in the production of crossover and flr singles. The X-ray-induced rate/wing anlage cell/Gy for flr singles was 1 X 10(-5), whereas that of crossover was 2 x 10(-4); the former and the latter rate are of the same order of magnitude as those of gene conversion and crossover in yeast, respectively. Thus, we conclude that proximal-marker "flr" singles induced in the transheterozygote are gene convertants. Using the model based on yeast that recombination events result from repair of double-strand breaks or gaps, we propose that mitotic recombination in the fly is a secondary result of recombinational DNA repair. Evidence for recombinational misrepair in the fly is given. The relative ratio of radiation-induced mitotic crossover to spontaneous meiotic crossover is one order of magnitude higher in the fly than in yeast and humans.

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“…The mutant UVS-1 (rec-, uvr +) which shows LHR after UV irradiation exhibits NLHE after gamma exposure [23], This shows that conclusions based on UV data may not hold true in case of ionising radiation. The diploid strain BZ34 used in this paper has been extensively investigated for the induction of non-reciprocal recombination by ionising radiations [14,21]. However, no gamma-sensitive mutants have been studied for LHR.…”

Section: Genetic Control Of Lhrmentioning

confidence: 99%

Liquid holding recovery in stationary and log phase cultures of diploid yeast exposed to gamma and alpha radiations

Reddy

Rao

Murthy

1976

Radiat Environ Biophys

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The kinetics of liquid-holding recovery (LHR) in diploid yeast after gamma and alpha irradiation is studied. In case of stationary phase culture the rate and extent of LHR is found to be greater for gamma-ray-induced damage than for alpha-ray-induced damage. At 10% survival level, the half-time for recovery is 5.2 h for gamma-ray damage and 12 h for alpha-ray damage. Further, while the recovery factor for alpha damage reaches saturation at 5% survival level, that for gamma damage continues to increase as survival level decreases. Oxygen is required for the recovery process during LH after gamma irradiation. The cells can recover to the same extent from both oxygen-dependent and oxygen-independent components of damage. Log phase cells containing a high per cent of budding cells, however, exhibit negative liquid holding effect after gamma irradiation.

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Induction of Heteroallelic Reversions and Lethality in Saccharomyces cerevisiae Exposed to Radiations of Various LET ( 60 Co g Rays, Heavy Ions, and π - Mesons) in Air and Nitrogen Atmospheres

Cited by 27 publications

References 0 publications

Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize

Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize

Induced rates of mitotic crossing over and possible mitotic gene conversion per wing anlage cell in Drosophila melanogaster by X rays and fission neutrons.

Liquid holding recovery in stationary and log phase cultures of diploid yeast exposed to gamma and alpha radiations

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