Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure

Ahmad, Iqbal; Hamid, Tariq; Fatima, Masroor; Chand, Hitendra S.; Jain, Swatantra Kumar; Athar, Mohammad; Raisuddin, Sheikh

doi:10.1016/s0304-4165(00)00098-2

Cited by 321 publications

(128 citation statements)

References 46 publications

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“…The oxidative metabolism of cells is a continuous source of ROS (resulting from the univalent reduction of O 2 ) that can damage most cellular components such as carbohydrates, lipids and proteins as well as promoting cell death (Ahmad et al, 2000;Morales et al, 2004). To protect themselves from these highly reactive intermediates, living organisms possess a biochemical defense system consisting of enzymatic and non-enzymatic antioxidants that scavenge any reactive species.…”

Section: Introductionmentioning

confidence: 99%

The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation

et al. 2014

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This study investigated the effects of the essential oil (EO) from Lippia alba on biochemical parameters related to oxidative stress in the brain and liver of silver catfish (Rhamdia quelen) after six hours of transport. Fish were transported in plastic bags and divided into three treatments groups: control, 30 µL L for three minutes), except for the control group. Fish transported in bags containing the EO did not have any alterations in acetylcholinesterase, ecto-nucleoside triphosphate diphosphohydrolase and 5'nucleotidase activity in the brain or superoxide dismutase activity in the liver. The hepatic catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), nonprotein thiol and ascorbic acid levels were significantly lower compared to the control group. However, the hepatic thiobarbituric acid-reactive substances, protein oxidation levels and the lipid peroxidation/catalase+glutathione peroxidase (LPO/CAT+GPx) ratio were significantly higher in fish transported with both concentrations of the EO, indicating oxidative stress in the liver. In conclusion, considering the hepatic oxidative stress parameters analyzed in the present experiment, the transport of previously sedated silver catfish in water containing 30 or 40 µL L -1 of EO from L. alba is less effective than the use of lower concentrations.Este estudo investigou os efeitos do óleo essencial (OE) de Lippia alba sobre parâmetros bioquímicos relacionados ao estresse oxidativo em cérebro e fígado de jundiá (Rhamdia quelen), após seis horas de transporte. Os peixes foram transportados em sacos plásticos e divididos em três tratamentos: controle, 30 μL L por três minutos), exceto para o grupo controle. Os peixes transportados em sacos contendo o OE não tiveram alterações na atividade da acetilcolinesterase (AChE), ecto-nucleosídeo trifosfato difosfohidrolase (NTPDase) e 5'nucleotidase, em cérebro ou superóxido dismutase (SOD) no fígado. O tiol não proteico (NPSH), os níveis de ácido ascórbico, catalase (CAT), glutationa-S-transferase (GST) e glutationa-peroxidase (GPx) hepáticos, foram significativamente mais baixos em comparação com o grupo controle. No entanto, as substâncias reativas ao ácido tiobarbitúrico (TBARS), os níveis de oxidação proteica e a taxa de peroxidação lipídica/catalase+glutationa peroxidase (LPO/ CAT+GPx) foram significativamente maiores nos peixes transportados com ambas as concentrações de OE, indicando estresse oxidativo no fígado. Em conclusão, considerando os parâmetros de estresse oxidativo do fígado analisados no presente experimento, o transporte de jundiás previamente sedados em água contendo 30 ou 40 µL L -1 de OE de L.alba é menos efetivo que utilizando concentrações menores.

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Section: Introductionmentioning

confidence: 99%

The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation

et al. 2014

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“…Should these antioxidant responses be overwhelmed by ROS, lipid peroxidation (LPO) and membrane damage will occur (Nakano et al, 1999). Indeed, the induction of these antioxidant responses and LPO have been commonly employed as biomarkers of oxidative stress (Di Giulio, 1991;Livingstone, 1993;Lackner, 1998;Ahmad et al, 2000) both under field and laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills

Woo

Liu

et al. 2006

Journal of Experimental Marine Biology and Ecology

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Chattonella marina, a red tide or harmful algal bloom species, has caused mass fish kills and serious economic loss worldwide, and yet its toxic actions remain highly controversial. Previous studies have shown that this species is able to produce reactive oxygen species (ROS), and therefore postulated that ROS are the causative agents of fish kills. The present study investigates antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to C. marina, compared with responses exposed to equivalent and higher levels of ROS exposure.Even though C. marina can produce a high level of ROS, gills and erythrocytes of sea bream exposed to C. marina for 1 to 6 h showed neither significant induction of antioxidant enzymes nor lipid peroxidation. Antioxidant responses and oxidative damage did not occur as fish mortality began to occur, yet could be induced upon exposure to artificially supplied ROS levels an order of magnitude higher. The result of this study implies that ROS produced by C. marina is not the principal cause of fish kills.

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“…CAT activity was assayed by the method with some modification as described by Ahmad et al, 2000). The assay mixture consisted of 0.1M phosphate buffer (pH 7.4), 0.019M hydrogen peroxide and 10% PMS in a final volume of 3 mL.…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

“…The enzyme activity was calculated as nmol CDNB conjugates/(min mg -1 protein) using amolar extinction coefficient of 9.6×103M −1 cm −1 . Glutathione peroxidase (GPx) activity was assayed according to the method described by Ahmad et al (2000) with some modifications. The assay mixture consisted of 0.1M phosphate buffer, 1mM EDTA, 1mM sodium azide, GR (1 IU mL -1 ), 1 mM GSH, 0.2 mM NADPH, 0.25 mM H 2 O 2 and PMS (10%) in a total volume of 2 mL.…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

Effect of battery-manufacturing effluent on endogenous antioxidant in freshwater fish

Ahmad

Ali

2013

Chemical Speciation & Bioavailability

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This study monitors and assesses the effect of battery-manufacturing effluent containing metals Pb, Zn and Cd on endogenous antioxidants. Malonaldehyde (MDA), reduced glutathione sulfyhydryl (GSH) and catalase (CAT) which are known biomarkers of effluent were exposed to 0%, 25%, 50%, 75% and 90% amendments for 74h on the gills, liver and kidneys of C. punctata. There was more metal Zn accumulation in the gills and GSH contents increased significantly in the gills (P<0.01), liver accumulation of Pb was found to be more (P<0.05), whereas lowest accumulation of Pb was found in kidneys and the highest accumulation of Cd (P<0.05). Over all amendments of the effluents, MDA contents were increased in the gills, liver and kidneys (P<0.01). GSH levels were decreased among the liver and kidneys compared to the gills (P<0.01) at 90% amendment. Effluent exposure caused a significant decrease in the activities of CAT in the gills, liver and kidneys (P<0.01, 0.05 and 0.05) of fish. Increased MDA activity was indicative of the formation of free radicals in the fish with exposure to amendments of battery manufacturing effluent, while increased levels of GSH pointed to the occurrence of a scavenging mechanism of free radicals.

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Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure

Cited by 321 publications

References 46 publications

The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation

The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation

Antioxidant responses and lipid peroxidation in gills and erythrocytes of fish (Rhabdosarga sarba) upon exposure to Chattonella marina and hydrogen peroxide: Implications on the cause of fish kills

Effect of battery-manufacturing effluent on endogenous antioxidant in freshwater fish

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