The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation

Salbego, Joseânia; Becker, Alexssandro Geferson; Gonçalves, Jamile Fabbrin; Menezes, Charlene; Heldwein, Clarissa G.; Spanevello, Rosélia Maria; Loro, Vânia Lúcia; Schetinger, Maria Rosa Chitolina; Morsch, Vera Maria; Heinzmann, Berta Maria; Baldisserotto, Bernardo

doi:10.1590/1982-0224-20130178

Cited by 37 publications

(41 citation statements)

References 44 publications

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“…The metabolic and antioxidant responses of fish exposed to anesthetics have been scarcely investigated (Azambuja et al, 2011;Becker et al, 2012Becker et al, , 2016Salbego et al, 2014Salbego et al, , 2015Toni et al, 2015). Transportation in closed systems leads to changes in the metabolic state of fish and may lead to oxidative damage through the production of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

“…In order to protect against oxidative stress, organisms have developed antioxidant defenses (Goyal et al, 2013) which include enzymes such as superoxide dismutase and catalase (Salbego et al, 2014. In addition, glutathione-S-transferase (GST) is an important detoxifying enzyme that is frequently induced under adverse conditions, such as poisoning.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, glutathione-S-transferase (GST) is an important detoxifying enzyme that is frequently induced under adverse conditions, such as poisoning. Activation of non-enzymatic antioxidant defenses, such as non-protein thiol groups (NPSH), are also important to aquatic organisms (Salbego et al, 2014.…”

Section: Introductionmentioning

confidence: 99%

“…Acetylcholinesterase (AChE) is an enzyme present in cholinergic synapses and at motor end plates, which is responsible for degrading acetylcholine (ACh) at the synaptic level, and is extremely important for many physiological functions in fish (Blenau et al, 2012;Salbego et al, 2014). The effect of anesthetics on AChE activity is poorly documented (Mazzanti et al, 1986;Lintern et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…N.E. Brown is also a suitable anesthetic and/or sedative for aquatic animals (Cunha et al, 2010b;Parodi et al, 2012;Salbego et al, 2014;Becker et al, 2016;Toni et al, 2015). Both extracts have been shown to be effective in minimizing the effects of transport stress in some fish species when added to the water during transportation (Cooke et al, 2004;Azambuja et al, 2011;Becker et al, 2012Becker et al, , 2016; however, the efficacy of EOL in altering biochemical parameters and oxidative stress apparently depends on the concentration administered (Salbego et al, 2014) and analysis of the effect of EUG on biochemical parameters after stress is limited.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

et al. 2017

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The transport of live fish is a routine practice in aquaculture and constitutes a considerable source of stress to the animals. The addition of anesthetic to the water used for fish transport can prevent or mitigate the deleterious effects of transport stress. This study investigated the effects of the addition of eugenol (EUG) (1.5 or 3.0 µL L -1) and essential oil of Lippia alba (EOL) (10 or 20 µL L -1 ) on metabolic parameters (glycogen, lactate and total protein levels) in liver and muscle, acetylcholinesterase activity (AChE) in muscle and brain, and the levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and nonprotein thiol groups (NPSH) and activity of glutathione-S-transferase in the liver of silver catfish (Rhamdia quelen; Quoy and Gaimard, 1824) transported for four hours in plastic bags (loading density of 169.2 g L -1). The addition of various concentrations of EUG (1.5 or 3.0 µL L -1 ) and EOL (10 or 20 µL L -1 ) to the transport water is advisable for the transportation of silver catfish, since both concentrations of these substances increased the levels of NPSH antioxidant and decreased the TBARS levels in the liver. In addition, the lower liver levels of glycogen and lactate in these groups and lower AChE activity in the brain (EOL 10 or 20 µL L -1 ) compared to the control group indicate that the energetic metabolism and neurotransmission were lower after administration of anesthetics, contributing to the maintenance of homeostasis and sedation status.Keywords: anesthetic, metabolism, stress, fish transportation. Parâmetros bioquímicos do jundiá (Rhamdia quelen) após transporte com eugenol ou óleo essencial de Lippia alba adicionado à águaResumo O transporte de peixes vivos é uma prática rotineira na aquicultura e constitui uma fonte considerável de estresse para os animais. A adição de anestésicos na água utilizada para o transporte de peixes pode prevenir ou mitigar os efeitos nocivos do estresse de transporte. Este estudo investigou os efeitos da adição de eugenol (EUG) (1,5 ou 3,0 mL L ) em parâmetros metabólicos (níveis de glicogênio, lactato e proteína total) no fígado e músculos, a atividade da acetilcolinesterase (AChE) no músculo e no cérebro, e os níveis de proteína carbonil (PC), ácido tiobarbitúrico (TBARS) e grupos tióis não proteicos (NPSH) e atividade da glutationa-S-transferase no fígado de jundiás (Rhamdia quelen; Quoy e Gaimard, 1824) transportados por quatro horas em sacos plásticos (densidade de carga de 169,2 g L -1). A adição de várias concentrações de EUG (1,5 ou 3,0 mL L ) na água de transporte é aconselhável para o transporte do jundiá, uma vez que ambas concentrações destas substâncias aumentaram os níveis do antioxidante NPSH e diminuíram os níveis de TBARS no fígado. Além disso, os níveis mais baixos de glicogénio e lactato no fígado destes grupos e menor atividade de AChE no cérebro (EOL 10 ou 20 mL L -1 ) em comparação com o grupo controle indicam que o metabolismo energético e neurotransmissão foram menores após a administração...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

et al. 2017

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Enhancing anesthesia and minimizing toxicity: evaluation of clove, Cinnamon, and tea tree essential oils in nile tilapia (Oreochromis niloticus)

Bona,

Passos,

Coppo

et al. 2024

Aquacult Int

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Pre-sedation and transport of Rhamdia quelen in water containing essential oil of Lippia alba: metabolic and physiological responses

Becker

Parodi

Zeppenfeld

et al. 2015

Fish Physiol Biochem

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The effects of transporting silver catfish (Rhamdia quelen) for 6 h in plastic bags containing 0 (control), 30 or 40 µL/L of essential oil (EO) from Lippia alba leaves were investigated. Prior to transport, the fish in the two experimental groups were sedated with 200 µL/L of EO for 3 min. After transport, dissolved oxygen, carbon dioxide, alkalinity, water hardness, pH, temperature and un-ionized ammonia levels in the transport water did not differ significantly among the groups. However, total ammonia nitrogen levels and net Na(+), Cl(-) and K(+) effluxes were significantly lower in the groups transported with EO of L. alba than those in the control group. PvO2, PvCO2 and HCO3(-) were higher after transporting fish in 40 µL/L of EO of L. alba, but there were no significant differences between groups regarding blood pH or hematocrit. Cortisol levels were significantly higher in fish transported in 30 µL/L of EO of L. alba compared to those of the control group. The metabolic parameters (glycogen, lactate, total amino acid, total ammonia and total protein) showed different responses after adding EO to the transport water. In conclusion, while the EO of L. alba is recommended for fish transport in the conditions tested in the present study because it was effective in reducing waterborne total ammonia levels and net ion loss, the higher hepatic oxidative stress in this species with the same EO concentrations reported by a previous study led us to conclude that the 10-20 µL/L concentration range of EO and lack of pre-sedation before transport are more effective.

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The essential oil from Lippia alba induces biochemical stress in the silver catfish (Rhamdia quelen) after transportation

Cited by 37 publications

References 44 publications

Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

Enhancing anesthesia and minimizing toxicity: evaluation of clove, Cinnamon, and tea tree essential oils in nile tilapia (Oreochromis niloticus)

Pre-sedation and transport of Rhamdia quelen in water containing essential oil of Lippia alba: metabolic and physiological responses

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