Induction of Hairy Roots on Cultivated Soybean Genotypes and Their Use to Propagate the Soybean Cyst Nematode

Savka, Michael A.

doi:10.1094/phyto-80-503

Cited by 76 publications

(61 citation statements)

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“…Susceptibility to agrocin 84 and uptake of agrocin 84 and of agrocinopines were determined as described elsewhere (22). Separation and detection of opines by HVPE was performed as reported previously (39).…”

Section: Methodsmentioning

confidence: 99%

Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport

et al. 1993

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The acc region, subcloned from pTiC58 of classical nopaline and agrocinopine A and B Agrobacterium tumefaciens C58, allowed agrobacteria to grow using agrocinopine B as the sole source of carbon and energy. acc is approximately 6 kb in size. It consists of at least five genes, accA through accE, as defined by complementation analysis using subcloned fragments and transposon insertion mutations of acc carried on different plasmids within the same cell. All

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Section: Methodsmentioning

confidence: 99%

Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport

et al. 1993

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“…Agrobacterium rhizogenes strain K599 (Savka et al, 1990) was transformed with the binary vector (plasmid pCAMBIA1301 containing GFP and with the DR5 promoter driving expression of the GUS gene, a gift of Senthil Subramanian) by electroporation and grown at 28°C on Luria-Bertani medium (10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, and 15 g of agar per liter) agar plates containing 30 mg L 2 1 rifampicin and 50 mg L 2 1 kanamycin. Five days after planting, a suspension of transformed Agrobacterium sp.…”

Section: Roles Of Ethylene and Auxinmentioning

confidence: 99%

Identification of the Primary Lesion of Toxic Aluminum in Plant Roots

et al. 2015

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Despite the rhizotoxicity of aluminum (Al) being identified over 100 years ago, there is still no consensus regarding the mechanisms whereby root elongation rate is initially reduced in the approximately 40% of arable soils worldwide that are acidic. We used high-resolution kinematic analyses, molecular biology, rheology, and advanced imaging techniques to examine soybean (Glycine max) roots exposed to Al. Using this multidisciplinary approach, we have conclusively shown that the primary lesion of Al is apoplastic. In particular, it was found that 75 µm Al reduced root growth after only 5 min (or 30 min at 30 µm Al), with Al being toxic by binding to the walls of outer cells, which directly inhibited their loosening in the elongation zone. An alteration in the biosynthesis and distribution of ethylene and auxin was a second, slower effect, causing both a transient decrease in the rate of cell elongation after 1.5 h but also a longer term gradual reduction in the length of the elongation zone. These findings show the importance of focusing on traits related to cell wall composition as well as mechanisms involved in wall loosening to overcome the deleterious effects of soluble Al.

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“…However, the vectors would have to actually function in soybean under a variety of welldefined conditions. An advantage for H. glycines research was that transformed soybean roots could be kept in tissue culture (Savka et al 1990;Cho et al 2001). In addition, modifications of those procedures have led to the development of chimeras that had transformed roots and untransformed aerial portions of the plant (Collier et al 2005;Klink et al 2008Klink et al , 2009b.…”

Section: An Alternative Strategy For Developmental Genomics-based Scrmentioning

confidence: 99%

“…The most efficient hairy root transformation procedure in soybean for H. glycines research relies on the bacterium, A. rhizogenes strain K599 (Haas et al 1995). Initial tissue culture-based analyses using the hairy root system have been employed in studying H. glycines development (Savka et al 1990;Cho et al 2001;Li et al 2010a). The hairy root technology could be used for overexpression, promoter analyses and RNAi studies Limpkins et al (2004).…”

Section: An Alternative Strategy For Developmental Genomics-based Scrmentioning

confidence: 99%

Cell-Specific Studies of Soybean Resistance to Its Major Pathogen, the Soybean Cyst Nematode as Revealed by Laser Capture Microdissection, Gene Pathway Analyses and Functional Studies

Klink¹,

Matsye²,

Lawrence³

2011

Soybean - Molecular Aspects of Breeding

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Induction of Hairy Roots on Cultivated Soybean Genotypes and Their Use to Propagate the Soybean Cyst Nematode

Cited by 76 publications

References 0 publications

Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport

Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport

Identification of the Primary Lesion of Toxic Aluminum in Plant Roots

Cell-Specific Studies of Soybean Resistance to Its Major Pathogen, the Soybean Cyst Nematode as Revealed by Laser Capture Microdissection, Gene Pathway Analyses and Functional Studies

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