Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles

Irani, Shiva; Shahmirani, Zhohreh; Atyabi, Seyed Mohammad; Mirpoor, Shahriar

doi:10.5114/aoms.2015.48221

Cited by 50 publications

(41 citation statements)

References 30 publications

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“…The treatment of SnO 2 NPs also showed significant morphological changes on cancer cell, which include cell membrane disruption, nuclear condensation, nuclear augmentation and finally significant percentage of cancer cell death. Similar results are reported, where NPs caused nuclear fragmentation, disintegration and cell death in cancer cells ,,,…”

Section: Resultsmentioning

confidence: 99%

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

et al. 2019

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Biocompatible SnO 2 NPs, were synthesized by simple and facile ultrasonic technique, and further studied for antibacterial, anticandidal and invitro cytotoxic activity. The obtained size of synthesized SnO 2 NPs, was 5-30 nm and characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). The evaluation of antibacterial and anticandidal activity of the SnO 2 NPs, was done by determining the minimum inhibitory concentration (MIC), and minimum bactericidal/fungicidal concentration (MBC/MFC), against Escherichia coli and Candida albicans, respectively. The MIC of 0.5 mg/ml and MBC of > 1 mg/ml for E. coli, while as the MIC of 8 mg/ml and MFC > 16 mg/ml for C. albicans, was obtained. Maximum activity for both, bacteria and Candida was achieved, however, to the best of our knowledge, this is the first report of anticandidal activity exhibited by SnO 2 NPs. Treatment of synthesized SnO 2 NPs against the colorectal (HCT-116) cancer cell line, decreased the cell survivability in a dose-dependent manner, as lower dose showed decrease of 73.00%, whereas, higher dosage caused a significant 31.34% decrease in the cell viability. The obtained results confirmed, that the prepared SnO 2 NPs can be the future broad-spectrum antibacterial, anticandidal and anticancer agent, and can be further explored for application in the biomedical field.promising potential for use in the pharmaceutical and biomedical applications. Supporting information summaryThe experimental section includes the detailed procedure for synthesis, characterization, antibacterial, anticandidal and cytotoxic activities of Tin Oxide NPs (SnO 2 NPs), which can be find in supporting information.

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Section: Resultsmentioning

confidence: 99%

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

et al. 2019

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“…The ROS intensity in glioblastoma cells showed the highest value in the conditions when the cells treated by cold plasma and AuNPs reached the lowest cell viability . Irani et al demonstrated the synergy of cold plasma and 55 nm AuNPs increased apoptotic cell death in colorectal cancer. These results are in agreement with the proposal by Kong et al…”

Section: Introductionmentioning

confidence: 99%

Cold Plasma Accelerates the Uptake of Gold Nanoparticles Into Glioblastoma Cells

Cheng

Rajjoub

Sherman

et al. 2015

Plasma Processes & Polymers

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The gold nanoparticles and cold plasma have been widely investigated separately in cancer therapy. In this paper, the two agents were combined in order to achieve a more profound understanding of their interactions in glioblastoma (U87) and normal human astrocytes (E6/E7). Gold nanoparticles were endocytosed at an accelerated rate in the U87 cell membrane due to the plasma treatment. This caused many divots across the cell membrane which made it more porous. On the contrary, in E6/E7 cells, there was no significant difference for penetration of gold nanoparticles or the cell membrane morphology. As proven in previous experiments the addition of the nanoparticles decrease cell viability, allowing the cold plasma to be more efficient. Present work demonstrated the synergistic effect of the two agents, which can be correlated in order to maximize the efficiency.

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“…Meanwhile, AuNPs are known to be readily manufactured and designed for targeting delivery of various compounds into cells. Therefore, they have emerged as a promising reagent, combined with CAP, for anti-cancer therapy in recent studies 4,9,10 . In our previous study, we explored the potential of a combination treatment of CAP with gold nanoparticles, which showed promising synergistic cytotoxicity to U373MG Glioblastoma multiforme (GBM) cells 11 .…”

Section: Introductionmentioning

confidence: 99%

Low dose Cold Atmospheric Plasma induces membrane oxidation, stimulates endocytosis and enhances uptake of nanomaterials in Glioblastoma multiforme cells

Liu

Scally

et al. 2019

Preprint

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Cold atmospheric plasma (CAP) has demonstrated synergistic cytotoxic effects with nanoparticles, especially promoting the uptake and accumulation of nanoparticles inside cells.However, the mechanisms driving the effects need to be explored. In this study, we investigate the enhanced uptake of theranostic nanomaterials by CAP. Numerical modelling of the uptake of gold nanoparticle into U373MG Glioblastoma multiforme (GBM) cells predicts that CAP may introduce a new uptake route. We demonstrate that cell membrane repair pathways play the main role in this stimulated new uptake route, following non-toxic doses of dielectric barrier discharge CAP (30 s, 75 kV). CAP treatment induces cellular membrane damage, mainly via lipid peroxidation as a result of reactive oxygen species (ROS) generation. Membranes rich in peroxidated lipids are then trafficked into cells via membrane repairing endocytosis. We confirm that the enhanced uptake of nanomaterials is clathrin-dependent using chemical inhibitors and silencing of gene expression. Therefore, CAP-stimulated membrane repair increases endocytosis and accelerates the uptake of gold nanoparticles into U373MG cells after CAP treatment. Our data demonstrate the utility of CAP to model membrane oxidative damage in cells and characterise a previously unreported mechanism of membrane repair to trigger nanomaterial uptake which will be useful for developing more efficient deliveries of nanoparticles and pharmaceuticals into cancer cells for tumour therapy and diagnosis. This mechanism of RONS-induced endocytosis will also be of relevance to other cancer therapies that induce an increase in extracellular RONS.

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Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles

Cited by 50 publications

References 30 publications

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

Cold Plasma Accelerates the Uptake of Gold Nanoparticles Into Glioblastoma Cells

Low dose Cold Atmospheric Plasma induces membrane oxidation, stimulates endocytosis and enhances uptake of nanomaterials in Glioblastoma multiforme cells

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