INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION

Laining, Asda; Usman, Usman; Syah, Rachman

doi:10.15578/iaj.11.1.2016.23-30

Cited by 4 publications

(7 citation statements)

References 20 publications

(24 reference statements)

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“…Hormon yang digunakan pada penelitian ini adalah salmon gonadotropin releasing hormon analog (sGnRH-a). Dosis yang dicobakan adalah dosis yang direkomendasikan oleh Laining et al (2016). Aplikasi hormon dilakukan secara injeksi sekali per minggu sebanyak 3x berdasarkan penelitian sebelumnya pada induk udang windu betina alam (Laining et al, 2015).…”

Section: Pendahuluanunclassified

“…Apabila induk jantan yang sama mengeluarkan lagi spermatofornya pada shock elektrik ke-2 dan ke-3, maka berturut-turut induk tersebut dikategorikan melakukan rematurasi ke-1 dan ke-2. Induk jantan mengeluarkan spermatofor dengan cara di-shock elektrik sebanyak tiga kali dengan interval satu minggu (Laining et al, 2016;Prasetyo et al, 2017).…”

Section: Pengamatan Perkembangan Spermatoforunclassified

“…Jumlah induk yang mengeluarkan spermatofor terbanyak pada fase maturasi, rematurasi ke-1 dan rematurasi ke-2 terjadi pada udang yang diinjeksi 0,2 mL/kg bobot udang (OV-2) diikuti OV-1 dan AB. Hasil ini berbeda dengan yang ditemukan Laining et al (2016) yang menyatakan penyuntikan sGnRH-a dengan dosis 0,1 mL/kg menghasilkan induk jantan (hasil budidaya) yang melepaskan spermatofor setelah diberikan kejutan listrik sebesar 80,8% lebih tinggi dibandingkan dosis 0,2 (46%) dan 0,3 (42,3%). Perbedaan tersebut diduga karena induk apkiran membutuhkan hormon gonadotropin yang lebih banyak dibandingkan induk yang tidak diapkir sehingga dosis yang dibutuhkan untuk perkembangan spermatofornya lebih tinggi.…”

Section: Performa Induk Jantan Pasca Injeksi Sgnrh-a Tahapunclassified

“…Hormon sintetis lainnya yang sudah secara luas diaplikasikan pada ikan dan krustase adalah kombinasi salmon gonadotropin releasing hormone analogue (sGnRH-a) dengan domperidone atau yang populer dikenal dengan nama ovaprim (Cejko et al, 2012;Genz et al, 2014;Jamroz et al, 2008;Zadmajid, 2016). Penelitian terkini pada udang windu jantan hasil budidaya menunjukkan bahwa hormon kombinasi sGnRH-a dan domperidone dapat diaplikasikan pada konsentrasi 0,1-0,2 mL/kg udang windu (Laining et al, 2016). Pada penelitian tersebut, injeksi pada konsentrasi 0,1 mL/kg diperoleh jumlah induk yang menghasilkan spermatofor sebanyak 80,8% lebih tinggi dari induk yang diablasi sebesar 46,1% dan pada dosis 0,2 mL/kg; berat spermatofor udang lebih besar (0,155 g) dibandingkan dosis lainnya.…”

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SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE STIMULASI PEMATANGAN SPERMATOFOR UDANG WINDU (Penaeus monodon) APKIRAN TANPA ABLASI

Trismawanti¹,

Nawang²,

Laining³

2018

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Penggunaan induk pada pembenihan udang windu pada dasarnya hanya untuk periode yang singkat dan selanjutnya induk udang windu diapkir baik betina maupun jantan. Tujuan penelitian ini untuk mengevaluasi efek injeksi hormon kombinasi salmon gonadotrophin releasing hormone analogue (sGnRH-a) dan domperidone terhadap karakteristik spermatofor induk udang windu, Penaeus monodon yang telah diapkir dari unit pembenihan tanpa ablasi tangkai mata. Dua tahap penelitian dilakukan pada udang windu jantan yang diapkir dari unit pembenihan. Tahap pertama terdiri atas dua dosis 0,1 (OV-1) dan 0,2 (OV-2) mL/kg bobot udang dan ablasi mata (AB) sebagai kontrol. Pada tahap ke-2, dosis yang diaplikasikan adalah dosis terbaik dari tahap ke-1 (OV-3) dan ablasi sebagai kontrol (AB). Induk jantan apkir ditebar masing-masing dengan kepadatan 14 ekor untuk tahap ke-1 dan 12 ekor pada tahap kedua. Injeksi dilakukan tiga kali dengan interval satu minggu untuk kedua percobaan tersebut. Seminggu setelah suntikan terakhir, induk jantan diberi kejutan listrik untuk mengeluarkan spermatofornya. Pada percobaan pertama, jumlah induk jantan yang mengeluarkan spermatofor pada maturasi dan rematurasi pertama lebih banyak ditemukan pada perlakuan OV-2 daripada OV-1 dan AB. Induk jantan pada perlakuan OV-3 pada tahap ke-2 menghasilkan lebih banyak spermatofor (58,3%) dibandingkan dengan AB (50%). Hubungan antara bobot badan dan bobot spermatofor untuk total data pada tahap ke-1 sifatnya linier dengan nilai r= 0,74 sedangkan hubungan antara bobot spermatofor dan jumlah spermatozoa sifatnya tidak linier dengan nilai r= 0.14. Induk apkiran jantan udang windu masih dapat memproduksi spermatofor melalui stimulasi injeksi hormon sGnRH-a pada dosis 0,2 mL/kg bobot udang relatif lebih banyak dibandingkan ablasi.Broodstocks in black tiger hatcheries are commonly used for only a short period of time. Both female and male broodstocks are discarded due to reduced spawning frequencies. This recent study was an effort to explore whether discarded broodstocks could be re-maturated without eyestalk ablation. For the research purpose, a two-stage experiment was conducted on discarded male tiger shrimps to evaluate the effect of sGnRH-a injection on the shrimp reproductive performances. In the first stage, the experiment consisted of injection treatment of different doses of sGnRH-a at 0.1 (OV-1) and 0.2 (OV-2) mL/kg and eyestalk ablation (AB) as a control. For the second stage, the treatment consisted of injection of sGnRH-a optimum dose obtained from the first trial (0.2 mL/kg shrimp (OV-3) and ablation as the control (AB). The treated male stocks were then randomly placed in concrete tanks with a density of 14 males/tank for the first stage and 12 males for the second stage. The injections were carried-out three times with an interval of one week for both stages. A week after the last injection, males were electrically shocked to release their spermatophores. In the first stage, the number of males releasing spermatophores at maturation and first re-maturation was higher in OV-2 treatment than that of OV-1 and AB treatments. In the second trial, the number of males in OV-3 treatment released more spermatophore (58.3%) compared to AB which was 50%. The body and spermatophore weights correlation of the discarded tiger shrimp in the first trial followed a positive linear with an r-value of 0.74. However, the spermatophore weight and the sperm cell number was not positively correlated with an r-value of 0.14. The discarded male tiger shrimps produced a relatively higher spermatophore when induced with sGnRHa at a dose of 0.2 mL/kg than those of shrimp treated with ablation.

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Section: Pendahuluanunclassified

Section: Pengamatan Perkembangan Spermatoforunclassified

Section: Performa Induk Jantan Pasca Injeksi Sgnrh-a Tahapunclassified

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SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE STIMULASI PEMATANGAN SPERMATOFOR UDANG WINDU (Penaeus monodon) APKIRAN TANPA ABLASI

Trismawanti¹,

Nawang²,

Laining³

2018

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“…Nevertheless, the captive male broodstock has not provided adequate results, such as poor spermatophore quality, which causes the broodstock to be unable to perform natural mating in a controlled pond or tank. The slow maturation of the captive male broodstock gonads is also the reason for low natural mating (Laining et al, 2016). Artificial breeding has been conducted to improve natural mating; however, hatchability was very low and, in some cases, even did not hatch.…”

Section: Introductionmentioning

confidence: 99%

Improved reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon with 17α-methyltestosterone induction

Rosmiati

Parenrengi

Suryati

et al. 2022

Israeli Journal of Aquaculture - Bamidgeh

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The purpose of this study was to evaluate the influence of different 17αmethyltestosterone injection frequencies at optimal doses to improve the quality of spermatophore and reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon. Broodstocks of tiger shrimp weighing 51-65 g for males and 86-161 g for females were collected from ponds and stored in a 3,000 m 3 concrete tank. Male broodstocks were injected with 17α-methyltestosterone at the optimized protocol at 300 ng/100 g body weight at different injection frequencies, three times, four times, and five times at seven-day intervals. As a control, male broodstocks were injected with a saline solution to determine the spermatophore quality. Evaluation of the reproductive performance of the captive male was observed in the captive female broodstock tiger shrimp using artificial insemination. The weight of spermatophore (0.041 g), gonadosomatic index (7.71%), the total number of spermatozoa (64 × 10 6 cells/mL) significantly increased, and the percentage of abnormal spermatozoa significantly decreased by 73% as well as hatching rate increased to 43.14% at the injection frequency of four times compared to the other treatments. In conclusion, injection of 17α-methyltestosterone at the dosage of 300 ng/100 g body weight at the frequency of giving four times was effective enough to improve the reproductive performance of captive tiger shrimp. Therefore, the method proposed can be a strategy to produce better larvae and increase the income of the aquaculture business.

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CAROTENOID-ENRICHED DIET FOR PREMATURATION STAGE OF POND- REARED TIGER SHRIMP, Penaeus monodon: Part II. EFFECT ON GONADAL MATURATION AND BIOCHEMICAL PROFILES OF OOCYTES, SPERMATOPHORES AND HEPATOPANCREAS

Laining

Trismawanti

Undu

et al. 2022

Indonesian Aquaculture Journal

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Two-phase feeding trials were conducted to evaluate the effect of the carotenoid mixture on gonadal development and biochemical properties of several tissues of tiger shrimp. The treatments were diet enriched with (PC) and without carotenoid mixture (PO). Shrimp with an initial body weight of 31.7±1.3 g were allocated among four of 1,000 m2 concrete ponds with a density of 1 shrimp/m2 and fed tested diets for five months. Selected shrimps from the pond with a minimum weight of 80 g for females and 60 g for males were stocked into four 10-m3 concrete tanks at 15 pairs per tank. Natural mating rate and ovary development were not stimulated by the carotenoid supplement. However, it significantly improved (P<0.05) both ovary maturation and spermatophore formation of tiger shrimp from 76.7±1.4 to 86.7±0.0% and from 69.9 ±4.5 to 82.3±4.0%, respectively. Total carotenoid content in meat, oocyte and hepatopancreas of female tiger shrimp significantly (P<0.05) increased by supplementing carotenoid compared to the control diet. The total amino acid content in the spermatophore of shrimp fed the PC diet was significantly higher (73.82%) than in the PO diet (66.09%). The present study revealed the important effect of carotenoid feed during the pre-maturation stage on the reproductive performances of pond-reared tiger shrimp.

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INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION

Cited by 4 publications

References 20 publications

SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE STIMULASI PEMATANGAN SPERMATOFOR UDANG WINDU (Penaeus monodon) APKIRAN TANPA ABLASI

SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE STIMULASI PEMATANGAN SPERMATOFOR UDANG WINDU (Penaeus monodon) APKIRAN TANPA ABLASI

Improved reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon with 17α-methyltestosterone induction

CAROTENOID-ENRICHED DIET FOR PREMATURATION STAGE OF POND- REARED TIGER SHRIMP, Penaeus monodon: Part II. EFFECT ON GONADAL MATURATION AND BIOCHEMICAL PROFILES OF OOCYTES, SPERMATOPHORES AND HEPATOPANCREAS

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