Induction of glycolipid biosynthesis by sodium butyrate in HeLa cells

Fishman, Peter H.; Simmons, James H.; Brady, Roscoe O.; Freese, Ernst

doi:10.1016/s0006-291x(74)80205-6

Cited by 113 publications

(33 citation statements)

References 8 publications

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“…subtilis: 13 nmol of glycine/(min A600), 3.3 nmol ATP/A655, and 221 nmol 02/(min A600); forE. coli: 35 nmol glycine/(min A600), 3.6 nmol of ATP/AooO and 273 nmol 02/(min A600).…”

Section: Resultsmentioning

confidence: 98%

Inhibitory Effects of Lipophilic Acids and Related Compounds on Bacteria and Mammalian Cells

Sheu

Salomon

Simmons

et al. 1975

Antimicrob Agents Chemother

155

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The inhibitory effect of lipophilic acids, antimicrobial food additives, and analgesics-antipyretics was examined at concentrations from 0.1 to 100 mM in bacteria ( Bacillus subtilis and Escherichia coli ) and mammalian cells (HeLa, human fibroblasts, and mouse neuroblastoma cells). Most compounds inhibit the growth of HeLa cells about as efficiently as that of B. subtilis . However, butyrate and propionate, as well as acetaminophen, antipyrene, phenacetin, and salicylamide, inhibit HeLa at millimolar concentrations whereas, at least 10 times higher concentrations are needed to inhibit B. subtilis . The concentrations needed to inhibit growth by 50% decrease with increasing octanol-water partition coefficients of the compound. Growth of E. coli is inhibited similar to that of B. subtilis by all compounds except butylbenzoate, decanoate, and linoleate which cannot penetrate the lipopolysaccharide layer. All growth inhibitors inhibit amino acid uptake into bacteria and their vesicles, and oxygen consumption in bacteria. In HeLa cells or human fibroblasts, neither amino acid uptake nor adenine 5′-triphosphate synthesis are inhibited by fatty acids at concentrations that completely inhibit growth. Short chain fatty acids (propionate, butyrate, and pentanoate) induce in HeLa the formation of cell processes. In neuroblastoma cells, grown in the presence of 10% fetal calf serum, butyrate also induces such processes which slowly continue to grow in length for at least 7 days; these processes differ in speed of formation, width, and cycloheximide susceptibility from the thin processes produced by serum deprivation alone.

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“…subtilis: 13 nmol of glycine/(min A600), 3.3 nmol ATP/A655, and 221 nmol 02/(min A600); forE. coli: 35 nmol glycine/(min A600), 3.6 nmol of ATP/AooO and 273 nmol 02/(min A600).…”

Section: Resultsmentioning

confidence: 98%

Inhibitory Effects of Lipophilic Acids and Related Compounds on Bacteria and Mammalian Cells

Sheu

Salomon

Simmons

et al. 1975

Antimicrob Agents Chemother

155

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“…As in other butyrate phenomena (13,17,20), the relative specificity of the butyrate effect is apparent because only the In contrast to the number of receptors, the basal and fluoride-stimulated activity of adenylate cyclase is not increased in butyrate-treated cells, which indicates that adenylate cyclase is a separate molecule from the f-adrenergic receptor and presumably under'independent regulatory control (24,25). At present, the adenylate cyclase from untreated and butyratetreated cells is being studied to examine other factors that might control the activity of the adenylate cyclase in HeLa cells or the efficiency of its coupling to the fl-adrenergic receptor.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, sodium butyrate has been found to induce various changes in mammalian cells in culture (13)(14)(15)(16)(17)(18)(19)(20).…”

mentioning

confidence: 99%

Induction of functional beta-adrenergic receptors in HeLa cells.

Tallman¹,

Smith²,

Henneberry³

1977

Proc. Natl. Acad. Sci. U.S.A.

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HeLa cells contain fl-adrenergic receptors that are characterized by specific binding of 1{3H]dihydroalprenolol, increased 3':5'-cyclic AMP production in intact cells after incubation with 1-isoproterenol, and increased adenylate cyclase [ATP pyrophosphate-lyase (cyclizing) (6,7) and supersensitivity after denervation and other manipulations (8, 9) and the role of modulators in the production of intracellular "second messengers."Because of the widespread distribution of fl-adrenergic receptors, which are important in catecholamine-mediated physiological events, and the apparent tight coupling between these receptors and adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.11, this system is a valuable model for the study of biochemical factors regulating receptors. Through the use of radioactive fl-adrenergic antagonists it has been possible to characterize unambiguously a binding site of the fl-adrenergic receptor in various cell types and to correlate its binding characteristics with studies of fl-receptor-dependent activation of adenylate cyclase (7,8,(10)(11)(12). Because it is possible to study the binding site of the fl-adrenergic receptor separately from the ability of fl-adrenergic agonists to activate adenylate cyclase, independent evaluation of the number of receptors and their ability to function can be made (12).To further elucidate the regulatory mechanisms controlling Abbreviation: cAMP, adenosine 3':5'-cyclic monophosphate.

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“…Butyrate is known to alter many processes in cultured cells, including his tone acetylation and phosphorylation [Boffa el al., 1981], DNA synthesis [Fallon and Cox, 1979], se cretion of sulfated glycoproteins [Heifitz and Prager, 1981], and production of sialyl-transferase [Fishman et al, 1974], Furthermore, Morrow et al [1983] found that butyrate altered the glycosylation of glycoprotein hormone a-subunit in glucose-starved Chang liver cells, perhaps by causing fucosylation of the glycopro tein. To eliminate the possibility that butyrate might be altering the composition of the hCG(3-like material produced by cervical carcinoma cells, we decided to omit butyrate from all incubations.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Production of hCGβ-Like Material by CaSki Human Cervical Carcinoma Cells in Roller Bottle Culture

et al. 1986

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This study was aimed at optimizing large-scale roller bottle culture conditions for CaSki human cervical carcinoma cells, to produce ectopic hCGβ-like material in quantities sufficient for subsequent characterization studies. Several cell culture techniques contributed to the achievement of this goal: (1) use of serum-free culture medium; (2) use of intermittent recovery periods in presence of serum; and (3) ultrafiltration of the serum-free medium pool for initial concentration of 100-fold.

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Induction of glycolipid biosynthesis by sodium butyrate in HeLa cells

Cited by 113 publications

References 8 publications

Inhibitory Effects of Lipophilic Acids and Related Compounds on Bacteria and Mammalian Cells

Inhibitory Effects of Lipophilic Acids and Related Compounds on Bacteria and Mammalian Cells

Induction of functional beta-adrenergic receptors in HeLa cells.

Optimization of the Production of hCGβ-Like Material by CaSki Human Cervical Carcinoma Cells in Roller Bottle Culture

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