Induction of GADD45α expression contributes to the anti-proliferative effects of polymethoxyflavones on colorectal cancer cells

Lee, Andy Chi Lung; Hsiao, Wei Chun; Wright, Duncan E.; Chong, Shin Yen; Leow, Siew Keng; Ho, Chi Tang; Kao, Cheng Fu; Lo, Yi Chen

doi:10.1016/j.jff.2013.01.003

Cited by 13 publications

(10 citation statements)

References 39 publications

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“…For the survival test, exponential-phase cultures were diluted to an appropriate concentration, and 200 cells were seeded onto plates containing various concentrations (10, 30, or 90 μM) of tangeretin; plates were then incubated for 3 days. The concentrations used were based on doses reported to be effective at treating cancer cells. , Relative survival rates of each experimental group were compared with the control group. Statistically significant differences between groups were analyzed by Student’s t -test, and differences were considered significant at p < 0.05.…”

Section: Methodsmentioning

confidence: 99%

Tangeretin Sensitizes SGS1-Deficient Cells by Inducing DNA Damage

Chong

2013

J. Agric. Food Chem.

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Tangeretin, a polymethoxyflavone found in citrus peel, has been shown to have antiatherogenic, anti-inflammatory, and anticarcinogenic properties. However, the underlying target pathways are not fully characterized. We investigated the tangeretin sensitivity of yeast (Saccharomyces cerevisiae) mutants for DNA damage response or repair pathways. We found that tangeretin treatment significantly reduced (p < 0.05) survival rate, induced preferential G1 phase accumulation, and elevated the DNA double-strand break (DSB) signal γH2A in DNA repair-defective sgs1Δ cells, but had no obvious effects on wild-type cells or mutants of the DNA damage checkpoint (including tel1Δ, sml1Δ mec1Δ, sml1Δ mec1Δ tel1Δ, and rad9Δ mutants). Additionally, microarray data indicated that tangeretin treatment up-regulates genes involved in nutritional processing and down-regulates genes related to RNA processing in sgs1Δ mutants. These results suggest tangeretin may sensitize SGS1-deficient cells by increasing a marker of DNA damage and by inducing G1 arrest and possibly metabolic stress. Thus, tangeretin may be suitable for chemosensitization of cancer cells lacking DSB-repair ability.

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Section: Methodsmentioning

confidence: 99%

Tangeretin Sensitizes SGS1-Deficient Cells by Inducing DNA Damage

Chong

2013

J. Agric. Food Chem.

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“…Recently, it was reported that nobiletin played an antitumor role. Nobiletin inhibits tumorigenesis and induces apoptosis of human cancer cells, including human osteosarcoma cells (8), human fibrosarcoma HT-1080 cells (9), and colorectal cancer cells (10). Nobiletin decreased the levels of phospho-ERK2 and phospho-AKT to attenuate metastasis in human cancer HepG2 cells (11).…”

Section: Highlightsmentioning

confidence: 99%

Nobiletin inhibits breast cancer via p38 mitogen-activated protein kinase, nuclear transcription factor-κB, and nuclear factor erythroid 2-related factor 2 pathways in MCF-7 cells

Liu

Wang

Tian

et al. 2018

Food & Nutrition Research

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IntroductionBreast cancer is one of the most commonly diagnosed cancers in women, with a high mortality rate.ObjectiveIn the present study, we evaluated the anticancer effect of nobiletin, a flavone glycoside, on the breast cancer cell line MCF-7.ResultCell viability and proliferation decreased and cell morphology changed from diamond to round after being treated with nobiletin. Nobiletin induced apoptosis of breast cancer MCF-7 cells via regulating the protein expression of Bax, Bcl-2, cleaved caspase-3, and p53. The expression of Bcl-2 decreased, while the expression of Bax and p53 increased in MCF-7 cells treated with nobiletin. Meanwhile, nobiletin inhibited cell migration by downregulating the protein expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). Moreover, phosphorylation of p38 was increased, and the translocation of p65 and nuclear factor erythroid 2-related factor 2 (Nrf2) to the nucleus was decreased, which suggested that the anticancer effects of nobiletin might at least partially rely on mediating the p38 mitogen-activated protein kinase, nuclear transcription factor-κB, and Nrf2 pathways in MCF-7 breast cancer cells.Conclusion and recommendationOur data showed that nobiletin was a potential antitumor drug, and it provided some experimental basis for the clinical application of tumor therapy.

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“…Lee et al [92] revealed that PMFmediated induction of GADD45α partially underlies the anti-proliferative effect of PMF on colorectal cancer cells. Nobiletin was found to act as an anti-carcinogenic compound through anti-proliferative activity, induction of apoptosis and cell cycle deregulation [93].…”

Section: Anti-proliferationmentioning

confidence: 99%

<i>Citrus</i> Flavonoids and Human Cancers

Ke¹,

Pan²,

Xu³

et al. 2015

JFNR

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Nowadays cancers pose a great threat to the health of human beings. Based on studies on the flavonoids and other bioactive compounds of Citrus fruits in current literature, it was widely suggested that the consumption of Citrus fruits is beneficial to the prevention and treatment of human chronic diseases including cancers. In the past decades, the study concerning Citrus flavonoids has covered various areas including the type, content and distribution of flavonoids in fruits; their variation between wild and cultivated genotypes; their antioxidant, antiinflammation, anti-aging, antimicrobial and anticarcinogenic activities. To enlighten future Citrus germplasm study, this review introduces briefly the functions of main types of Citrus flavonoids, including antioxidant, antiinflammation and anti-aging activities, and their relationships to human cancers. Most importantly, the mechanisms of action by which Citrus flavonoids play their roles in human cancer prevention and treatment were summarized.

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Induction of GADD45α expression contributes to the anti-proliferative effects of polymethoxyflavones on colorectal cancer cells

Cited by 13 publications

References 39 publications

Tangeretin Sensitizes SGS1-Deficient Cells by Inducing DNA Damage

Tangeretin Sensitizes SGS1-Deficient Cells by Inducing DNA Damage

Nobiletin inhibits breast cancer via p38 mitogen-activated protein kinase, nuclear transcription factor-κB, and nuclear factor erythroid 2-related factor 2 pathways in MCF-7 cells

<i>Citrus</i> Flavonoids and Human Cancers

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