Induction Of Dna Synthesis In Lymphocytes In Vitro By Various Bacteria, With Special Reference To Neisseria Gonorrhoeae, In Patients With Uro-Arthritis (Reiter's Disease)

Rosenthal, L; Danielsson, Dan

doi:10.3109/03009747809098845

Cited by 8 publications

(6 citation statements)

References 18 publications

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“…Pathologic specimens suggested that events similar to those noted at mucosal surfaces occurred after dissemination. For example, gonococci have been seen inside A cells of the synovial membrane (14), and gonococcal peptidoglycan monomers have been implicated in cell damage at synovial membranes (42,47 …”

Section: S109mentioning

confidence: 99%

Gonococcal and meningococcal pathogenesis as defined by human cell, cell culture, and organ culture assays

Stephens

1989

Clin Microbiol Rev

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Human cells, cell cultures, and organ cultures have been extremely useful for studying the events that occur when gonococci and meningococci encounter human mucosal surfaces. The specificity and selectivity of these events for human cells are striking and correlate with the adaptation of these pathogens for survival on human mucous membranes. To colonize these sites, meningococci and gonococci have developed mechanisms to damage local host defenses such as the mucociliary blanket, to attach to epithelial cells, and to invade these cells. Attachment to epithelial cells mediated by pili, and to some types of cells mediated by PIIs, serves to anchor the organism close to sources of nutrition and allows multiplication. Intracellular invasion, possibly initiated by the major porin protein, may provide additional nutritional support and protection from host defenses. Mucosal invasion may also result in access of gonococci and meningococci to the bloodstream, leading to dissemination.

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Section: S109mentioning

confidence: 99%

Gonococcal and meningococcal pathogenesis as defined by human cell, cell culture, and organ culture assays

Stephens

1989

Clin Microbiol Rev

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“…Gonococci have many antigens in common with meningococci. Rosenthal and Danielsson (1978) found a close antigenic relationship between these microorganisms. Therefore the possibility of an immune response developing as a result of meningococcal infection cannot be excluded.…”

Section: Discussionmentioning

confidence: 81%

“…The bacterial antigens were prepared by Dan Danielsson, Orebro, Sweden. The procedures used have been described in detail elsewhere (Rosenthal and Danielsson, 1978). PPD tuberculin was obtained from Statens Seruminstitut, Copenhagen, as a solution of 1 mg/ml in phosphate buffer, without addition of preservative.…”

Section: Bacterial Antigensmentioning

confidence: 99%

Lymphocyte reactivity in patients with gonococcal urethritis.

Rosenthal¹,

Sandström²

1978

Sexually Transmitted Infections

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SUMMARY Lymphocyte reactivity to virulent gonococcal antigen T2 and the non-pathogenic Neisseria pharyngis (NPN) has been studied by using the 14C-thymidine uptake in cell cultures from 42 patients with gonococcal urethritis and from 18 controls. The DNA synthesis in cell cultures with T2 antigen was higher in 21 female patients than in the 18 controls. No differences in DNA synthesis were observed in antigen-stimulated cell cultures from patients with single or multiple infections, from patients with urogenital complications, or from controls. Gonococcal antibodies in the serum were detected by the gonococcal complement-fixation test (GCFT). A study of the possible correlation between the outcome of the serological test and the cellular response to gonococcal antigen showed that 14C-thymidine uptake in lymphocyte cultures from male patients with negative GCFT, stimulated with T2 antigen, was much lower than the thymidine uptake in stimulated cell cultures from all the other male and female patients (P<0 001). The DNA synthesis was higher in cell cultures from seronegative women than from seronegative men (P<00 1). A significant difference (P<001) was also noted in the lymphocyte reactivity to gonococcal antigen between controls and all patients, except in those men who gave negative results to the serological tests. There were no differences between these two groups with respect to the thymidine uptake in NPN-stimulated cell cultures.

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“…A dilution of 1:500 of this antigen was found to be optimal in pilot tests. Later on, one strain of Neisseria gonorrhoeae of colony morphology type T2 whole cells was prepared as described elsewhere (Rosenthal and Danielsson, 1978). at a concentration of approximately 2 x 106 per culture.…”

Section: Methodsmentioning

confidence: 99%

“…Triplicate cell cultures containing 3 x 105 E cells/tube with and without antigen were set up. E 0-1 ,uCi of '4C-thymidine in 0 05 ml of phosphate buffered saline was added to each culture on day 5, and the radioactivity was measured in an Intertechnique (Nano teknik, Sweden) liquid scintillation X~c ounter (Rosenthal and Danielsson, 1978).…”

Section: Methodsmentioning

confidence: 99%