Induction of cytosine-rich poly(A)+ RNAs in Chlamydomonas reinhardtii by cell wall removal

Su, Xing; Kaska, Deborah D.; Gibor, Aharon

doi:10.1016/0014-4827(90)90115-q

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Second, GLEtreated vegetative protoplasts proceed to synthesize and assemble new cell walls within 2 hr (Robinson and Schlosser, 1978). As first shown by Adair and Apt (1990) and Su et al (1990), this response initiates with the upregulation of wallspecific mRNA synthesis, facilitating the molecular identification of wall-specific genes.…”

Section: Introductionmentioning

confidence: 83%

“…VSP-1 mRNA levels were extremely low in vegetative cells, increased during the first 2 hr following GLE treatment of the cells, and then declined once wall regeneration was completed, as shown in Figure 2. This pattern of expression, in response to GLE treatment, is a diagnostic feature of a V wall structural protein-encoding sequence (Adair and Apt, 1990;Su et al, 1990).…”

Section: Vsp Cdna Clonesmentioning

confidence: 97%

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Isodityrosine Cross-Linking Mediates Insolubilization of Cell Walls in Chlamydomonas

Waffenschmidt¹,

Woessner²,

Beer³

et al. 1993

The Plant Cell

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Enzymatic removal of the cell wall induces vegetative Chlamydomonas reinhardtii cells to transcribe wall genes and synthesize new hydroxyproline-rich glycoproteins (HRGPs) related to the extensins found in higher plant cell walls. A cDNA expression library made from such induced cells was screened with antibodies to an oligopeptide containing the (SP)x repetitive domains found in Chlamydomonas wall proteins. One of the selected cDNAs encodes an (SP)x-rich polypeptide that also displays a repeated YGG motif. Ascorbate, a peroxidase inhibitor, and tyrosine derivatives were shown to inhibit insolubilization of both the vegetative and zygotic cell walls of Chlamydomonas, suggesting that oxidative cross-linking of tyrosines is occurring. Moreover, insolubilization of both walls was concomitant with a burst in H2O2 production and in extracellular peroxidase activity. Finally, both isodityrosine and dityrosine were found in hydrolysates of the insolubilized vegetative wall layer. We propose that the formation of tyrosine cross-links is essential to Chlamydomonas HRGP insolubilization.

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Section: Introductionmentioning

confidence: 83%

Section: Vsp Cdna Clonesmentioning

confidence: 97%

Isodityrosine Cross-Linking Mediates Insolubilization of Cell Walls in Chlamydomonas

Waffenschmidt¹,

Woessner²,

Beer³

et al. 1993

The Plant Cell

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show abstract

“…No VSP-3 m R N A is detected in zygotes. In gametes and asynchronous vegetative cells, levels of wallspecific transcripts are extremely low: gamete lytic enzyme (GLE) treatment of vegetative cells causes an induction of wall-specific mRNAs [ 1,48]. Figure 6E shows a time-course analysis of VSP-3 m R N A levels in vegetative cells recovering from GLE treatment.…”

Section: Gene Expression Studiesmentioning

confidence: 99%

Domain conservation in several volvocalean cell wall proteins

et al. 1994

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Domain conservation in several volvocalean cell wall proteinsWoessner, J.P.; Molendijk, A.J.; van Egmond, P.; Klis, F.M.; Goodenough, U.W.; Haring, M.A. Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Key words: cell wall, HRGPs, domain conservation, exon shuffling, protein evolution, Chlamydomonas AbstractBased on our previous work demonstrating that (SerPro)x epitopes are common to extensin-like cell wall proteins in Chlamydomonas reinhardtii, we looked for similar proteins in the distantly related species C. eugametos. Using a polyclonal antiserum against a (SerPro)l 0 oligopeptide, we found distinct sets of stage-specific polypeptides immunoprecipitated from in vitro translations of C. eugarnetos RNA. Screening of a C. eugametos cDNA expression library with the antiserum led to the isolation of a cDNA (WP6) encoding a (SerPro)×-rich multidomain wall protein. Analysis of a similarly selected cDNA (VSP-3) from a C. reinhardtii cDNA expression library revealed that it also coded for a (SerPro)x-rich multidomain wall protein. The C-terminal rod domains of VSP-3 and WP6 are highly homologous, while the N-terminal domains are dissimilar; however, the N-terminal domain of VSP-3 is homologous to the globular domain of a cell wall protein from VoIvox carteri. Exon shuffling might be responsible for this example of domain conservation over 350 million years of volvocalean cell wall protein evolution.

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Volvocine cell walls and their constituent glycoproteins: an evolutionary perspective

Woessner

Goodenough

1994

The Protistan Cell Surface

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Induction of cytosine-rich poly(A)+ RNAs in Chlamydomonas reinhardtii by cell wall removal

Cited by 7 publications

References 22 publications

Isodityrosine Cross-Linking Mediates Insolubilization of Cell Walls in Chlamydomonas

Isodityrosine Cross-Linking Mediates Insolubilization of Cell Walls in Chlamydomonas

Domain conservation in several volvocalean cell wall proteins

Volvocine cell walls and their constituent glycoproteins: an evolutionary perspective

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