Induction of Controlled Wound Healing with PMMA Segments in the Deep Stroma in Corneas of Hens

Merayo‐Lloves, Jesús; Blanco–Mezquita, T.; Ibares‐Frías, Lucía; Fabiani, L.; Alvarez-Barcia, Angel; Martinez-Garcia, C.

doi:10.1177/112067211002000108

Cited by 10 publications

(15 citation statements)

References 57 publications

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“…The wound-healing response around the channel after PMMA rings in this animal model was previously described [48]. Histologically, several findings underline the biocompatibility of both groups, since there was no evidence of an inflammatory response at 30 and 90 days.…”

Section: Groups/ Treatmentsupporting

confidence: 64%

“…Furthermore, the proportions of hen cornea layer [44,45] and the fibrillar collagen arrangement [46,47] are very similar to those of human cornea. Also, in previous experiments the hen was used as animal model to study the wound-healing response after intracorneal ring segment implantation by our group, so that the learning curve was already realized [48].…”

Section: Groups/ Treatmentmentioning

confidence: 99%

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Clinical and pathological effects of different acrylic intracorneal ring segments in corneal additive surgery

et al. 2010

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a b s t r a c tThe objective of this work was to evaluate the potential use of less stiff materials based on acrylic copolymers of methyl methacrylate/2-ethylhexyl acrylate (MMA/EHA) as devices to correct, stabilize and improve the effect of poly(methyl methacrylate) (PMMA) intracorneal ring segments. MMA/EHA and PMMA intracorneal ring segments were surgically implanted in the corneas of Lohmann Classic hens. The effects of the intracorneal ring segments were assessed by optical measurements and corneal tolerance was evaluated through biomicroscopic examination over a 90-day observation period and by conventional histology. The experimental results demonstrated that the intracorneal ring segments made of MMA/EHA copolymers provided a significant change in the corneal curvature and an improved in vivo response compared to those obtained for PMMA rings, which was attributed to the higher flexibility of the copolymeric materials, indicating that these systems might be considered suitable as an alternative to those currently used, for application in clinical practice.

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Section: Groups/ Treatmentsupporting

confidence: 64%

Section: Groups/ Treatmentmentioning

confidence: 99%

Clinical and pathological effects of different acrylic intracorneal ring segments in corneal additive surgery

et al. 2010

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“…Wound healing after ICRS implantation has been studied in different animal models, such as rabbits [17] and hens [22]. In our current study, we used the hen as an animal model because we could adapt the materials of human ICRS implantation to them.…”

Section: Discussionmentioning

confidence: 99%

“…Eleven time points after ICRS implantation or after channel preparation in control group 1 were examined histologically at 4, 12, 24, 48, and 72 hours, 7 days, and 1, 2, 3, 4 and 6 months. These times were chosen based on previous wound healing studies in hens and rabbits [17,22,23]. Enucleated eyes were fixed with 10 % buffered formalin and embedded in paraffin.…”

Section: Clinical Coursementioning

confidence: 99%

“…Most of the histological studies after ICRS implantation have been carried out in human corneal tissue after complications or when the cornea was removed for keratoplasty [18][19][20][21]. Histopathological studies in animals have been performed in rabbits [17] and hens [22]. However, there are relatively few studies that have reported the complete histological changes that occur during the wound healing process of both human and animal corneas [17,18,20,21].…”

Section: Introductionmentioning

confidence: 99%

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Tissue reaction after intrastromal corneal ring implantation in an experimental animal model

Ibares‐Frías

Gallego

Cantalapiedra-Rodríguez

et al. 2015

Graefes Arch Clin Exp Ophthalmol

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Purpose To evaluate corneal wound healing in the hen animal model after additive surgery with an intracorneal ring segment (ICRS). Methods We implanted one ICRS in each eye of 76 hens. In control group 1 (n=22 hens), the stromal channel was prepared but no ICRS was inserted. In control group 2 (n=2 hens), no surgery was performed. Animals were randomly separated into groups and euthanized after clinical follow-up of 4 and 12 hours, 1, 2, 3, and 7 days, and 1, 2, 3, 4, and 6 months. Corneas were stained with hematoxylin-eosin. Apoptosis was measured by terminal uridine nick end-labeling assays. Cell proliferation and myofibroblast-like differentiation were assayed by BrdU and α-smooth muscle actin immunofluorescence microscopy. Stromal matrix changes were documented by electron microscopy.Results Epithelial and stromal cell apoptosis around the ICRS-implanted and control group 1 eyes peaked at 12 hours, but continued for 72 hours. In ICRSimplanted eyes, epithelial and stromal proliferation was present at 12 and 24 hours, respectively, and peaked at 7 days and 72 hours, respectively. Some proliferation in the ICRS-implanted group continued through the 6-month follow-up, and myofibroblast-like cells differentiated one to three months after ICRS implantation. The segments rotated within the stroma as the limbal inferior angle approached the epithelium. Conclusions Wound healing after ICRS implantation in hen corneas was similar to that of other corneal surgical wounds in stages. However, there were some specific features related to the small size of the epithelial wound and the device permanently implanted inside the cornea.

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A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds

et al. 2021

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Induction of Controlled Wound Healing with PMMA Segments in the Deep Stroma in Corneas of Hens

Abstract: Wound healing was induced in the deep corneal stroma by means of PMMA segment implantation in a new animal model developed in hens.

Cited by 10 publications

References 57 publications

Clinical and pathological effects of different acrylic intracorneal ring segments in corneal additive surgery

Clinical and pathological effects of different acrylic intracorneal ring segments in corneal additive surgery

Tissue reaction after intrastromal corneal ring implantation in an experimental animal model

A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds

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