A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds

Ulag, Songul; Şahin, Ali; Güncü, Mehmet Mucahit; Aksu, Burak; Ekren, Nazmi; Şengör, Mustafa; Kalaskar, Deepak M.; Gündüz, Oğuzhan

doi:10.1016/j.bprint.2021.e00173

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…[ 22 ]. Since the amount of PLA is high, the samples loaded with J (10–30%) have almost the same FT-IR spectra as the pristine PLA fiber, this being also in good agreement with other studies [10.3390/polym6010093] [ 23 ].…”

Section: Resultssupporting

confidence: 90%

Fabrication of Electrospun Juglans regia (Juglone) Loaded Poly(lactic acid) Scaffolds as a Potential Wound Dressing Material

et al. 2022

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Juglone (5-hydroxy-1,4-naphthoquinone) (J) is a naphthoquinone structured allelochemical that is mostly found in the roots, leaves, nut-hulls, bark, and wood of walnut (Juglans regia). In this study, the biocompatibility, mechanical, thermal, chemical, morphological, and antimicrobial properties of the poly(lactic acid) (PLA) (w/v)/J (10, 20, 30 mg) electrospun scaffolds were investigated. Based on the results of the study, it was shown that juglone addition increased the antimicrobial properties of the scaffolds against the Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), compared to the neat PLA film after 24 h of contact time. According to the tensile test results, the addition of J made the scaffolds more flexible but decreased the mechanical strength. The cytotoxicity properties of the J-added scaffolds demonstrated a toxic behavior on the first day of incubation. However, with an increase in the J ratio, the fibroblast cell metabolic activity increased for all incubation periods.

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Section: Resultssupporting

confidence: 90%

Fabrication of Electrospun Juglans regia (Juglone) Loaded Poly(lactic acid) Scaffolds as a Potential Wound Dressing Material

et al. 2022

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“…Peaks at 1083 and 1112 cm −1 indicate the presence of CF bonds. [ 37,38 ] In Figure 3A(d), the FTIR spectrum of the VAN showed characteristic peaks at 3277 cm −1 of OH − stretch and 1626 cm −1 of C and O stretch, 1525 cm −1 of C. [ 39 ] In Figure 3B(e), the FTIR spectrum of the PVA/GEL had a nearly equal spectrum with pure PVA with a peak at 1324 cm −1 . With the GEL addition, it can be observed that the absorption band at 3277 cm −1 shifted to 3270 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug‐Loaded Nanofibrous Patches

Cesur

Ilhan

Pilavci

et al. 2023

Macro Materials & Eng

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Corneal ulcer, which is brought on by a breach in the epithelial barrier, is a dangerous infection of the avascular corneal stroma. New treatment strategies are needed, suppressing the aggressive nature of the disease and including a combination of different drugs. In this study, vancomycin (VAN) and fluconazole (FLU) dual‐drug loaded dual‐layered polyvinyl alcohol and gelatin (PVA/GEL) nanofibrous patches are produced by electrospinning. Scanning electron microscopy (SEM) images show smooth surfaces are obtained for both pure and drug‐loaded nanofibrous patches. The tensile test results report that loading the FLU and VAN separately into the PVA/GEL patches decrease both the tensile strength and elongation at break and it is further reduced when combining two drug‐loaded layers in one patch. According to drug release results, the FLU and VAN‐loaded nanofibrous patches show a controlled release profile extending up to 96 h. Moreover, PVA/GEL/FLU, PVA/GEL/VAN, and PVA/GEL/FLU/VAN nanofibrous patches display significant antimicrobial activity against Candida albicans and Staphylococcus aureus. SEM, 4'‐6diamidynofenyloindol (DAPI) staining, and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay show that PVA/FLU and PVA/GEL/FLU/VAN nanofibrous patches have a superior effect on NIH3T3 cell spreading and proliferation. The novelty of this study lays in the development of a potential dual drug rapid treatment for corneal ulcers of aggressive nature.

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“…Instead of focusing on adding epithelial-originated cells, it was suggested that adding patterns to the surface of keratoprosthesis tends to restore functional epithelial coverage more efficiently (Brunette et al, 2017). In 2021, Ulag S et al reported a novel design of 3D printed PMMA/vancomycin (VAN) scaffolds to treat the Thiel-Behnke corneal dystrophy (Ulag et al, 2021). The scaffold consists of honeycomb structures that would allow better cell adhesion Frontiers in Bioengineering and Biotechnology frontiersin.org with ameliorated proliferation of cells (Ulag et al, 2021).…”

Section: Cell Involvementmentioning

confidence: 99%

“…In 2021, Ulag S et al reported a novel design of 3D printed PMMA/vancomycin (VAN) scaffolds to treat the Thiel-Behnke corneal dystrophy (Ulag et al, 2021). The scaffold consists of honeycomb structures that would allow better cell adhesion Frontiers in Bioengineering and Biotechnology frontiersin.org with ameliorated proliferation of cells (Ulag et al, 2021). However, further investigation is required as the antimicrobial activity of scaffold is not able to cover the full device due to undesired permeability of the materials (Ulag et al, 2021).…”

Section: Cell Involvementmentioning

confidence: 99%

“…The scaffold consists of honeycomb structures that would allow better cell adhesion Frontiers in Bioengineering and Biotechnology frontiersin.org with ameliorated proliferation of cells (Ulag et al, 2021). However, further investigation is required as the antimicrobial activity of scaffold is not able to cover the full device due to undesired permeability of the materials (Ulag et al, 2021). But it is anticipated that the characterized and quantified biochemical and biophysical cues of the anterior basement membrane of the cornea will facilitate epithelial coverage of the implant, as topographic cues impact essential phases of the corneal epithelial wound-healing process, translating into an improved rate of wound healing with subsequent stratification and maintenance of a healthy epithelium (Shen et al, 2017).…”

Section: Cell Involvementmentioning

confidence: 99%

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Advances in 3D bioprinting technology for functional corneal reconstruction and regeneration

Jia

Lau

et al. 2023

Front. Bioeng. Biotechnol.

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Corneal transplantation constitutes one of the major treatments in severe cases of corneal diseases. The lack of cornea donors as well as other limitations of corneal transplantation necessitate the development of artificial corneal substitutes. Biosynthetic cornea model using 3D printing technique is promising to generate artificial corneal structure that can resemble the structure of the native human cornea and is applicable for regenerative medicine. Research on bioprinting artificial cornea has raised interest into the wide range of materials and cells that can be utilized as bioinks for optimal clarity, biocompatibility, and tectonic strength. With continued advances in biomaterials science and printing technology, it is believed that bioprinted cornea will eventually achieve a level of clinical functionality and practicality as to replace donated corneal tissues, with their associated limitations such as limited or unsteady supply, and possible infectious disease transmission. Here, we review the literature on bioprinting strategies, 3D corneal modelling, material options, and cellularization strategies in relation to keratoprosthesis design. The progress, limitations and expectations of recent cases of 3D bioprinting of artifial cornea are discussed. An outlook on the rise of 3D bioprinting in corneal reconstruction and regeneration is provided.

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A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds

Cited by 8 publications

References 28 publications

Fabrication of Electrospun Juglans regia (Juglone) Loaded Poly(lactic acid) Scaffolds as a Potential Wound Dressing Material

Fabrication of Electrospun Juglans regia (Juglone) Loaded Poly(lactic acid) Scaffolds as a Potential Wound Dressing Material

A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug‐Loaded Nanofibrous Patches

Advances in 3D bioprinting technology for functional corneal reconstruction and regeneration

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