Induction of collagenase production in Vibrio B-30

Abstract

The inducible nature of an extracellular collagenase produced by a marine Vibrio (Vibrio B-30, ATCC 21250) was demonstrated by observing the increase in extracellular collagenase activity after the addition of collagen to cell cultures in the latter part of the exponential growth phase. When collagenase-hydrolyzed collagen was added, the lag time required before collagenase production was detected decreased significantly compared with cultures receiving collagen. Cells

Purification and Characterization of an Extracellular Protease from the Fish Pathogen Yersinia ruckeri and Effect of Culture Conditions on Production

“…The production of the 47-kDa protease was influenced by the composition of the culture medium: growth and protease production were optimum in peptone medium whereas activity was not detected when the microorganism was grown in the presence of Casamino Acids, suggesting that intact peptides are necessary in the induction process. A similar behavior has been observed for A. salmonicida (9), Aeromonas liquefaciens (9), Serratia marcescens (4), Vibrio species (12), and E. chrysanthemi (41). By contrast, in the case of A. hydrophila, peptone was the best medium for protease production, although growth was not too prolific (34).…”

Proteinases of psychrotrophic bacteria: their production, properties, effects and control

“…The organism also produces an extracellular collagenase induced by peptone and collagen (or a high molecular weight fragment: Long et al 1981;Reid et al 1980;Keil-Dlouha et al 1976). The polar helical portion of the collagen molecule appears to be the true physiological inducer of collagenase (Dreisbach & Merkel, 1978). The collagenase is repressed by some amino acids, ammonium ions and glucose (Long et al 1981).…”

A polyphasic approach to the exploration of collagenolytic activity in the bacterial community associated with the marine sponge Cymbastela concentrica

“…For example, isolate I's 16S rRNA gene sequence is 99% identical to those of Vibrio crassostreae, which has been reported a pathogen of oysters (Faury et al, 2004) and Vibrio splendidus, which causes disease in turbot larvae. Other Vibrio and Bacillus species have also been reported to contain collagenase genes with potential roles in disease (Dreisbach & Merkel, 1978;Smith & Merkel, 1982;Mäkinen & Mäkinen, 1987;Lund & Granum, 1999).…”

“…They can cause tissue destruction and thus facilitate the invasion of a pathogen into internal parts of host tissue (Smith & Merkel, 1982;Harrington, 1996). The presence of collagenolytic bacteria in the marine environment is more widely distributed than previously thought (Dreisbach & Merkel, 1978;Takeuchi et al, 1992;Thomas et al, 2008). For example, Merkel et al (1975) found that 44% of marine isolates obtained from coastal waters were capable of producing collagenolytic enzymes.…”