Induction of chromosomal aberrations by the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl in cultured human lymphocytes

Abstract

The genotoxicity of the rhodium(III) complex cis-[Rh(biq)(2)Cl(2)]Cl (complex R) in cultured human lymphocytes was studied using the chromosome aberrations (CAs) assay. Lymphocyte cultures were initiated from two adult healthy non-smoking male volunteers and were exposed to the complex for a duration of 3 or 20 h prior to cell collection. The reduction in mitotic indices (MI) and the induction of CAs represented the toxic and clastogenic effects of the different treatments, respectively. Complex R proved to be an intermediate toxic clastogen with a MI(50) of 1.0 microg/ml and a minimum positive dose (MPD) of 0.1 microg/ml. Like bleomycin, complex R exerted its clastogenic effects without the need for metabolic activation and induced CAs of all types in lymphocytes treated in the G(2) and late S phases and, therefore, can be considered a radiomimetic. In addition, it induced more total CAs of chromatid-type than of chromosome-type. The reduction in the frequencies of CAs following the 20 h treatment as compared with those induced following the 3 h treatments indicated that human lymphocytes in the presence of complex R can partially tolerate the lesions involved in CA production. Based on the biological effects of complex R and the similarities between its functional group and that of bleomycin, possible mechanisms for complex R genotoxicity are discussed.

Mutagenicity and DNA-damaging potential of clenbuterol and its metabolite 4-amino-3,5-dichlorobenzoic acid in vitro

“…Since 4-ADBA was solved in 0.01% DMSO, the latter was used as a solvent control. A lymphocyte culture treated with 10 μg/ml bleomycin for 3 h in vitro (Sadiq et al, 2000) served as a positive control.…”

“…Solvent control was the lymphocyte culture incubated with DMSO (0.01%). Positive control was the lymphocyte culture treated with bleomycin in concentration 10 μg/ml for 3 h in vitro (Sadiq et al, 2000).…”

Genotoxicity assessment in iron deficiency anemia patients using sister chromatid exchanges and chromosomal aberrations assays

“…In the last 2 h of incubation, Colcemid (0.1 g/mL) was added to arrest the cells in metaphase. Cultured cells were harvested and fixed as previously described [20,21]. The cellular suspension was then dropped on prechilled microscope slides to obtain metaphase spreads.…”

The Effects of Pegylated Interferon Alpha-2a and Alpha-2b Therapy on Chromosomal Aberrations and Mitotic Index in Patients with Chronic Hepatitis B

“…19 Cytogenetic analysis of metaphase chromosome spreads in point of chromosomal aberrations and analysis of mitotic index in cultured cells can be used for detecting the genotoxic effect of an agent. 20,21 Chromosomal aberration is a term that used to define deletion, duplication, translocation and breakage of chromosomes. Mitotic index (MI) is a measure for the proliferation status of a cell population.…”