Induction of benzo (a)pyrene monooxygenase in fish and the salmonella test as a tool for detecting mutagenic/carcinogenic xenobiotics in the aquatic environment

Kurelec, Branko; Matijašević, Zdenka; Rijavec, M.; Alačević, Marija; Britvić, Smiljana; Müller, Wernér E.G.; Ζahn, Rudolf K.

doi:10.1007/bf01685508

Cited by 61 publications

(12 citation statements)

References 15 publications

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“…The Salmonella/microsome test has been used by Ames et al (1975) and Paolini et al (1991) to assess the potentially mutagenic and carcinogenic intermediate metabolites resulting from xenobiotic biotransformation. The adaptation of "sh liver homogenates to the Salmonella/microsome (Kurelec et al, 1979;RodrmH guezAriza et al, 1995) and SOS-umu tests (Bihari et al, 1990) has been reported, although not yet extensively characterized.…”

Section: Introductionmentioning

confidence: 96%

Benzo[a]pyrene and β-Naphthoflavone Mutagenic Activation by European Eel (Anguilla anguilla L.) S9 Liver Fraction

Maria

Correia

Santos

2002

Ecotoxicology and Environmental Safety

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Is Anguilla anguilla L. (eel) liver ethoxyresorufin O-deethylase (EROD) induction absolutely necessary in order to convert promutagens as benzo[a]pyrene (BaP) into a mutagenic compound? Eels were exposed for 8 h to clean (control) and 0.3 microM beta-naphthoflavone (BNF)-contaminated water. The 8-h exposure to 0.3 microM BNF brought about a very high EROD induction (10 pmol/min/mg protein) compared to control animals (1 pmol/min/mg protein). The Ames test (Maron and Ames, 1983) was carried out with Salmonella typhimurium TA 98 strain (TA98 His-) and eel isolated S9 liver fraction was used as a metabolic BaP activator. The BaP and BNF dose range concentrations tested were 0 (blank), 0.015, 0.08, 0.15, 0.38, 0.75, 1.5, 3.8, and 7.5 microM/plate and 0 (blank), 0.412, 1.235, 3.704, 11.1, 33.0, and 100 nM BNF, respectively. A dose-response relationship between BaP concentration and mutagenic activity was observed in the presence of S9 fractions in control and 0.3 microM BNF-exposed eels. Significant positive results, as TA98 His+ revertants, were observed at 0.38, 0.75, 1.5, 3.8, and 7.5 microM BaP/plate induced by BNF S9 fractions. Significant BaP mutagenic activation by liver control S9 was detected only at 1.5, 3.8, and 7.5 microM/plate. The BaP 1.5, 3.8, and 7.5 microM/plate mutagenic activation by BNF S9 and control S9 were not significantly different. Relative to BNF activation, it was only possible to detect His+ reversion at 11.1 nM BNF concentration with 0.3 microM BNF-induced S9. The above results demonstrate that the eel S9 liver fraction has the capacity to biotransform high BaP concentrations and convert it into a mutagenic compound with or without previous liver BNF biotransformation induction. The same does not apply to low BaP concentrations, where liver S9 induction by BNF is necessary to promote mutagenesis.

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Section: Introductionmentioning

confidence: 96%

Benzo[a]pyrene and β-Naphthoflavone Mutagenic Activation by European Eel (Anguilla anguilla L.) S9 Liver Fraction

Maria

Correia

Santos

2002

Ecotoxicology and Environmental Safety

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“…Sites S-3 and S-5 are the reference sites of low environmental stress, while S-l and S-2, the industryhrban sewage sites (the latter load will be reduced soon due to a functional sewer system to be introduced at the end of 1997), are known to be most exposed to genotoxic xenobiot~cs at the area selected. The state of induction of benzo(a)-pyrene monooxygenase (BaPMO) activity in livers of the fish species Blennius pavo ranges from 1.6 (reference sites) to 36.7 pm01 BaPOH min-' mg-' (industry/ urban sewage sltes) (Kurelec et al 1977); frequency of chromosomal aberrations In Mytilus galloprovincialis from 2.9 (reference sltes) to 9.6% (industryhrban sewage s~tes) (AI- Sabt~ & Kurelec 1985); mixed functional oxygenase (MFO) induction in the livers of carp Cyprinus carpjo using hexane extracts from reference sites, 2.1 to 46.2 pm01 BaPOH min-' mg-' (industry/ urban sewage sites) (Kurelec et al 1982); mutagenicity/carcinogenicity testing (Ames test) revealed only for the industryhrban sewage sites 60% revertants of Salmonella typhimurium (Kurelec et al 1979).…”

Section: Discussionmentioning

confidence: 99%

Accumulation of cadmium and zinc in the marine sponge Suberites domuncula and its potential consequences on single-strand breaks and on expression of heat-shock protein:a natural field study

Müller¹,

Batel²,

Lacorn³

et al. 1998

Mar. Ecol. Prog. Ser.

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The marine sponge Suberites domuncula (Porifera; Demospongiae) was used in a natural field study (in the vicinity of Rovinj, Croatia) as a bioindicator to assess the genotoxic risk of cadmium exposure for sponges as a model for invertebrates, and as an early stress marker of expression of heatshock protein 70 (M, 73 kDa; termed HSP73). Slgn~ficant differences in 'natural' cadmium levels of S. domunculd were found among sponges collected from differently polluted sites; in contrast zlnc levels were relatively similar. Sponges llving at polluted sites contained 15 to 24 times more cadmlum than sponges at unpolluted reference sites. The capacity of S. domuncula to accumulate cadmlun~ after an add~tlonal exposure to 5 mg I-' of cadmium for 5 d, was markedly higher (increase in cadmium content of 4 2 to 49 mg Cd kg-') for sponges collected from polluted sites compared to those from uncontaminated or less contaminated sites (increase of 8 to 29 mg Cd kg-'); a strong increase in cadmlum level was accompanied by a reduction in zinc level in the sponges from polluted sites. In this study we have used the rapid, sensitive and cheap Fast ~i c r o m e t h o d~ to determine the number of DNA single-stl-and breaks in S. domuncula collected from differently polluted sites and some of those sponges additionally exposed to cadmium. The strand scission factor (SSF) was found to depend on the pollution load; it further increased by 1.7-to 8.6-fold after additional exposure to cadmium (5 mg I-'). Similarly, the levels of HSP73 were found to be highest in S. domuncula living at polluted sites. Additional exposure of the sponges to cadmium strongly increased HSP73 level. This increase in HSP73 level was higher in sponges from polluted sites compared to those collected from uncontaminated or less contaminated control sites. From these data we conclude that the sponge S. domuncula is a useful bioindicator regarding the cadmium load of the aquatic environment.

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“…several species of fish have been shown to metabolize BP to products that are mutagenic to bacteria (Ahokas et al, 1977;Hintonet ~., 1978;Kurelec et al, 1979;Payne et al, 1979;Stegeman et al, 1978). In ------fact, hepatic microsomes from untreated European lake trout, scup and winter flounder activate BP to about the same extent as hepatic micro somes from PCB-induced rats.…”

Section: )mentioning

confidence: 99%

Xenobiotic monooxygenase activity and the response to inducers of cytochrome P-450 during embryonic and larval development in fish

Binder¹

1981

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Induction of benzo (a)pyrene monooxygenase in fish and the salmonella test as a tool for detecting mutagenic/carcinogenic xenobiotics in the aquatic environment

Cited by 61 publications

References 15 publications

Benzo[a]pyrene and β-Naphthoflavone Mutagenic Activation by European Eel (Anguilla anguilla L.) S9 Liver Fraction

Benzo[a]pyrene and β-Naphthoflavone Mutagenic Activation by European Eel (Anguilla anguilla L.) S9 Liver Fraction

Accumulation of cadmium and zinc in the marine sponge Suberites domuncula and its potential consequences on single-strand breaks and on expression of heat-shock protein:a natural field study

Xenobiotic monooxygenase activity and the response to inducers of cytochrome P-450 during embryonic and larval development in fish

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