Xenobiotic monooxygenase activity and the response to inducers of cytochrome P-450 during embryonic and larval development in fish

Binder, Robert L.

doi:10.1575/1912/2414

Cited by 3 publications

(2 citation statements)

References 82 publications

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“…This level of contamination could be responsible for the apparent induction of cytochrome P-4SØ-linked activities in this species as it has been sufficient in some other fish systems (Binder, 1982).…”

Section: No Difference Was Found In Preliminary Reconstitutionmentioning

confidence: 89%

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

Klotz¹

1983

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Section: No Difference Was Found In Preliminary Reconstitutionmentioning

confidence: 89%

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

Klotz¹

1983

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“…Some metabolism assays were conducted in controlled atmospheres rather than under air, according to Binder (1982) with modifications. In these reactions, microsomes, buffer, and substrate were combined and the mixture was bubbled gently with either N2 or CO for 60 seconds.…”

Section: Louis Mo) Analysis By Gc/ecd and Gc/ms Revealed No Hydrophmentioning

confidence: 99%

Tetrachlorobiphenyl metabolism, toxicity, and regulation of cytochrome P50 expression in a marine teleost fish

White¹

1994

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The effects of 3,3',4,4'-tetrachlorobiphenyl (TCB) were examined in the marine fish scup (Stenotomus chrysops), focusing on the interactions between TCB and the CYP1A1 enzyme system. A low TCB dose (0.1 mg/kg) elicited strong and sustained induction of hepatic CYPlA1 mRNA, protein content, and catalytic activity. A high TCB dose (5.0 mg/kg) elicited similar, strong induction of hepatic CYPlA1 mRNA, but not of CYPlA1 protein content or catalytic activity. This post-transcriptional "suppression" at the high TCB dose was specific for CYPlA1, and was not seen with other hepatic enzymes. In vitro studies indicate that hepatic microsomal CYP A1 is inactivated in the presence of TCB plus cofactor, likely due to the production of reactive oxygen species during TCB occupation of the active site. CYPlA1 inactivation by TCB in vitro may explain the TCBelicited suppression of CYPlA1 protein content in vivo.Both TCB doses elicited strong CYPlA1 induction in vascular endothelium of all organs, which was sustained for several weeks. Induction in intestinal epithelia was stronger at the high TCB dose, but induction in epithelia of liver, kidney, and gill were stronger at the low TCB dose. Both TCB doses caused proliferation of endoplasmic reticulum in liver, renal tubule necrosis, depletion of hematopoietic tissue in kidney, hyperplasia of gill epithelia, and increased number of melanomacrophage aggregates in spleen. The high dose induced tail fin erosion, affecting both epithelial and calcified bone tissue. Tissue alterations were more severe at the high TCB dose, and repair of lesions occurred by day 16 at the low dose. The high dose caused mortality of many individuals.The two TCB congeners 3,3',4,4'-TCB and 2,2',5,5'-TCB were each converted to aqueous-soluble metabolites by hepatic microsomes from scup, beluga whale, and pilot whale. Induction response, correlation analysis, and inhibition studies indicate that 3,3',4,4'-TCB is metabolized by scup CYP1Al, and 2,2',5,5'-TCB by the putative scup CYP2B. Correlation analysis and inhibition studies suggest that 3,3',4,4'-TCB is metabolized by cetacean CYP1A. Both cetacean species expressed microsomal proteins that are immunochemically related to mammalian CYP2B forms. Cytochrome P450 systems from both cetacean species are partially characterized here.

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Xenobiotics in gametes of Lake Michigan lake trout (Salvelinus namaycush) induce hepatic monooxygenase activity in their offspring*1

Binder

Lech

1984

Fundamental and Applied Toxicology

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Xenobiotic monooxygenase activity and the response to inducers of cytochrome P-450 during embryonic and larval development in fish

Cited by 3 publications

References 82 publications

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops

Tetrachlorobiphenyl metabolism, toxicity, and regulation of cytochrome P50 expression in a marine teleost fish

Xenobiotics in gametes of Lake Michigan lake trout (Salvelinus namaycush) induce hepatic monooxygenase activity in their offspring*1

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