Induction of autophagy via the TLR4/NF-κB signaling pathway by astragaloside Ⅳ contributes to the amelioration of inflammation in RAW264.7 cells

Ying, Yanling; Sun, Chongde; Zhang, Siqi; Chen, Bojun; Yu, Jing-Ze; Liu, Feiyu; Wen, Jing; Hou, Jiong; Han, Si-si; Yan, Jin; Yang, Zhongshan; Xiong, Lei

doi:10.1016/j.biopha.2021.111271

Cited by 23 publications

(12 citation statements)

References 29 publications

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“…TLR4 is the upstream regulator of the NF-κB signaling pathway [22]. TLR4 promotes the release of cytokines, chemokines, and costimulatory molecules which are proinflammatory transcription factors, playing a crucial role in inflammation [23][24][25][26]. Nevertheless, in STAD, the mechanism of ASCL2 in inflammation via TLR4 activation is not fully understood.…”

Section: Discussionmentioning

confidence: 99%

Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma

Zheng

Cai

et al. 2022

World J Surg Onc

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Background To investigate the role of achaete-scute complex-like 2 (ASCL2) in stomach adenocarcinoma (STAD), we analyze whether ASCL2 suppression could retard cancer development and further observe the relevance between ASCL2 and inflammation via Toll-like receptor 4 (TLR4) activation in STAD, both in vitro and in vivo. Methods Proliferation, development, inflammation, and apoptosis in STAD are observed using sh-ASCL2 lentivirus via TLR4 activation in vitro and in vivo. The relationship between ASCL2 and inflammation is analyzed. Western blotting of ASCL2 with the target protein of immune-associated cells is performed. The prognosis of STAD and associated ASCL2 mutation are analyzed. Results The ASCL2 level in STAD tumor tissues is increased, compared to normal tissues, and brings a worse prognosis. The ASCL2 shows a negative correlation with inflammation, and TLR4 reveals a positive correlation with gastric cancer. ASCL2 expression is high in MGC803 cells. Sh-ASCL2 could reduce STAD development by decreasing proliferation, tumor volume, and biomarker levels and increasing apoptosis in vitro and in vivo. The inflammatory role of ASCL2 is regulated through TLR4 activation. ASCL2 levels may be related to CNTNAP3, CLIP1, C9orf84, ARIH2, and IL1R2 mutations; positively correlated with M2 macrophage and T follicular helper cell levels; negatively correlated with neutrophil, dendritic cell, monocyte, CD8 T cell, and M1 macrophage levels; and involved in STAD prognosis. Conclusions The ASCL2 may adjust inflammation in STAD through TLR4 activation and may be associated with related immune cells. ASCL2 is possibly an upstream target factor of the TLR4 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma

Zheng

Cai

et al. 2022

World J Surg Onc

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“…Ying et al reported that the inhibition of macrophage autophagy exacerbated inflammatory injury and inflammatory cytokine release, while Astragaloside IV induced macrophage autophagy by inhibiting the TLR4/NF-kB pathway, thereby reducing the inflammatory response and lung injury (156). The administration of NF-kB inhibitor further contributed to protein expression of autophagy, this suggested that the TLR4/NF-kB signaling pathway negatively regulates autophagy (156). Wang et al found that the SIRT6 activator UBCS039 enhanced autophagy and M2 polarization in macrophages of a sepsis-induced ALI model, thereby reducing lung injury, while autophagy inhibitors eliminated this effect (157).The results found that SIRT6 overexpression restrained mTOR pathway activation, suggesting involvement of the mTOR pathway in SIRT6regulated macrophage autophagy and M2 polarization (157).…”

Section: Macrophage Autophagy Aggravates Ali/ardsmentioning

confidence: 99%

“…Ying et al. reported that the inhibition of macrophage autophagy exacerbated inflammatory injury and inflammatory cytokine release, while Astragaloside IV induced macrophage autophagy by inhibiting the TLR4/NF-kB pathway, thereby reducing the inflammatory response and lung injury ( 156 ). The administration of NF-κB inhibitor further contributed to protein expression of autophagy, this suggested that the TLR4/NF-κB signaling pathway negatively regulates autophagy ( 156 ).…”

Section: The Modulation Of Macrophage Autophagy Is a Promising Strate...mentioning

confidence: 99%

Progress in preclinical studies of macrophage autophagy in the regulation of ALI/ARDS

2022

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Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a critical clinical syndrome with high morbidity and mortality that poses a major challenge in critical care medicine. The development of ALI/ARDS involves excessive inflammatory response, and macrophage autophagy plays an important role in regulating the inflammatory response in ALI/ARDS. In this paper, we review the effects of autophagy in regulating macrophage function, discuss the roles of macrophage autophagy in ALI/ARDS, and highlight drugs and other interventions that can modulate macrophage autophagy in ALI/ARDS to improve the understanding of the mechanism of macrophage autophagy in ALI/ARDS and provide new ideas and further research directions for the treatment of ALI/ARDS.

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“…The source plants are mainly distributed in Shanxi, Inner Mongolia and Gansu in China (Fu et al, 2014). AR has immune‐enhancing (Wang et al, 2012), anti‐tumor (Li, He, et al, 2020; Li, Sun, et al, 2020), anti‐inflammatory (Ying et al, 2021), hepatoprotective (Zou et al, 2021), antioxidant (Wang, Ding, et al, 2021), antiviral (Liang et al, 2019) and other effects, and is widely used in drugs, cosmetics, food, health products and beverages (Napolitano et al, 2013). AR appears most frequently in herbal prescriptions for the prevention of New Coronary Pneumonia (COVID‐19) and has received a lot of attention from scholars (Chinsembu, 2020).…”

Section: Introductionmentioning

confidence: 99%

Combining network pharmacology with chromatographic fingerprinting and multicomponent quantitative analysis for the quality evaluation of Astragali Radix

Guo

Sha

et al. 2022

Biomedical Chromatography

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Nowadays, cultivated variants and adulterants of Astragali Radix (AR) have flooded the market, causing the quality assurance of AR to be challenging. To address this issue, we combined network pharmacology with chromatographic fingerprinting and multicomponent quantitative analysis for the quality evaluation of AR. Specifically, through network pharmacology, a complete understanding of the active components and pharmacological activities of AR was established. In addition, establishing fingerprint profiles and multicomponent quantitation by high-performance liquid chromatography (HPLC) is convenient and comprehensive, and can more fully reflect the overall situation of the distribution of various chemical components. To evaluate and differentiate AR from different origins, hierarchical cluster analysis and principal component analysis were performed. The result showed that AR acts synergistically through multiple targets and pathways. The content of chemical components in AR from different origins varied significantly. Combining network pharmacology and multicomponent quantification results, astragaloside II and IV and formononetin can be used as quality markers for the quality control of AR. This study provides a comprehensive and reliable strategy for the quality evaluation of AR and identifies its quality markers to ensure the quality of the herb.

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Induction of autophagy via the TLR4/NF-κB signaling pathway by astragaloside Ⅳ contributes to the amelioration of inflammation in RAW264.7 cells

Cited by 23 publications

References 29 publications

Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma

Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma

Progress in preclinical studies of macrophage autophagy in the regulation of ALI/ARDS

Combining network pharmacology with chromatographic fingerprinting and multicomponent quantitative analysis for the quality evaluation of Astragali Radix

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