Induction of apoptosis in Vero cells by<i>Aeromonas veronii</i>biovar<i>sobria</i>vacuolating cytotoxic factor

Martins, Luciano Moura; Catani, Cleide Ferreira; Falcón, Rosabel; Carbonell, Gleize Villela; Azzoni, Adriano A.; Yano, Tomomasa

doi:10.1111/j.1574-695x.2006.00176.x

Cited by 16 publications

(19 citation statements)

References 25 publications

(44 reference statements)

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“…sobria. The toxin was recently partially purified and appears to be a 60-kDa nonhemolytic enterotoxin that acts as a serine protease and causes apoptosis in Vero cells (199). It can be neutralized partially by antibodies produced against aerolysin.…”

Section: Organotrophic Diseasementioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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SUMMARY Over the past decade, the genus Aeromonas has undergone a number of significant changes of practical importance to clinical microbiologists and scientists alike. In parallel with the molecular revolution in microbiology, several new species have been identified on a phylogenetic basis, and the genome of the type species, A. hydrophila ATCC 7966, has been sequenced. In addition to established disease associations, Aeromonas has been shown to be a significant cause of infections associated with natural disasters (hurricanes, tsunamis, and earthquakes) and has been linked to emerging or new illnesses, including near-drowning events, prostatitis, and hemolytic-uremic syndrome. Despite these achievements, issues still remain regarding the role that Aeromonas plays in bacterial gastroenteritis, the extent to which species identification should be attempted in the clinical laboratory, and laboratory reporting of test results from contaminated body sites containing aeromonads. This article provides an extensive review of these topics, in addition to others, such as taxonomic issues, microbial pathogenicity, and antimicrobial resistance markers.

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Section: Organotrophic Diseasementioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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“…Some of these examples include the works on the cloning, expression, and purification of the virulence-associated protein D from Xylella fastidiosa (Catani et al, 2004), a very important citric fruit pathogen associated to orange plantation production and financial harm in Brazil, where the cloning technology was applied while characterizing the virulence factors associated to the orange trees and fruit diseases and also the purification of the Vacuolating Cytotoxic Factor (VCF) of Aeromonas veronii biovar sobria (Martins et al, 2007), an enterotoxic cytotoxin associated to cases of human enteric diseases. IMAC showed to be extremely important to the development of these scientific works as in the first case we described proteins structured to present poly histidine tags and in the second case we presented a toxin having natural ion metallic affinity to cooper.…”

Section: Examples Of Imac Applied In Our Workmentioning

confidence: 99%

Ion Metallic Affinity Chromatography and Purification of Bacterial Toxin

Martins¹,

Yano²

2012

Affinity Chromatography

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“…Surprisingly, the pathogenic yersiniae can induce apoptosis, necrosis, and pyroptosis depending on the host-cell type infected (Monack et al, 1997; Ruckdeschel et al, 1997, 1998, 2001; Bergsbaken and Cookson, 2007; Zheng et al, 2012). Similarly, Aeromonas species are also able to induce apoptosis (through various caspase activation) (Galindo et al, 2004b, 2006a,b; Martins et al, 2007; Su et al, 2007; Sierra et al, 2007, 2010). …”

Section: Introductionmentioning

confidence: 99%

Modulation of host immune defenses by Aeromonas and Yersinia species: convergence on toxins secreted by various secretion systems

Rosenzweig

Chopra

2013

Front. Cell. Infect. Microbiol.

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Like other pathogenic bacteria, Yersinia and Aeromonas species have been continuously co-evolving with their respective hosts. Although the former is a bonafide human pathogen, the latter has gained notararity as an emerging disease-causing agent. In response to immune cell challenges, bacterial pathogens have developed diverse mechanism(s) enabling their survival, and, at times, dominance over various host immune defense systems. The bacterial type three secretion system (T3SS) is evolutionarily derived from flagellar subunits and serves as a vehicle by which microbes can directly inject/translocate anti-host factors/effector proteins into targeted host immune cells. A large number of Gram-negative bacterial pathogens possess a T3SS empowering them to disrupt host cell signaling, actin cytoskeleton re-arrangements, and even to induce host-cell apoptotic and pyroptotic pathways. All pathogenic yersiniae and most Aeromonas species possess a T3SS, but they also possess T2- and T6-secreted toxins/effector proteins. This review will focus on the mechanisms by which the T3SS effectors Yersinia outer membrane protein J (YopJ) and an Aeromonas hydrophila AexU protein, isolated from the diarrheal isolate SSU, mollify host immune system defenses. Additionally, the mechanisms that are associated with host cell apoptosis/pyroptosis by Aeromonas T2SS secreted Act, a cytotoxic enterotoxin, and Hemolysin co-regulated protein (Hcp), an A. hydrophila T6SS effector, will also be discussed.

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Induction of apoptosis in Vero cells byAeromonas veroniibiovarsobriavacuolating cytotoxic factor

Cited by 16 publications

References 25 publications

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

Ion Metallic Affinity Chromatography and Purification of Bacterial Toxin

Modulation of host immune defenses by Aeromonas and Yersinia species: convergence on toxins secreted by various secretion systems

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