Induction of apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus

Hu, Yan‐Wei; Liu, Chunyu; Du, Chong-Min; Zhang, Jian; Wu, Wei; Z, Gu

doi:10.1016/j.jep.2009.03.016

Cited by 86 publications

(59 citation statements)

References 28 publications

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“…Plant derived drugs have a profound impact on anticancer research, where drugs like vinblastine, vincristine, taxol and camptothecin have improved survival in patients undergoing chemotherapy for some types of cancer. The continuing search for new anticancer compounds in plants and traditional food is a realistic and promising strategy for its prevention (Hu et al 2009). Numerous chemical groups with anticancer properties are derived from plants including alkaloids, phenylpropanoids and terpenoids (Park et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

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Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor activity. Extract/fractions were prepared and in vitro cytotoxicity was assessed using SRB assay. Most effective fractions were subjected to fluorescence microscopy based acridine orange/ethidium bromide (AO/EB) and Hoechst 33342 staining to determine apoptosis induction and DNA fragmentation assay. Comet and micronuclei assay were performed to assess genotoxicity. Cell cycle analysis was also performed. In vivo anti-tumor potential was evaluated by Ehrlich ascites carcinoma (EAC) model in mice. The alcoholic stem bark extract of M. elengi along with four fractions showed potential in vitro cytotoxicity in SRB assay. Of these, dichloromethane and ethyl acetate fractions were selected for further studies. The fractions revealed apoptosis inducing potential in AO/EB and Hoechst 33342 staining, which was further confirmed by DNA fragmentation assay. Genotoxic potential was revealed by comet and micronuclei assay. Fractions also exhibited specific cell cycle inhibition in G 0 /G 1 phase. In EAC model, ethyl acetate fraction along with the standard (cisplatin) effectively reduced the increase in body weight compared to control and improved mean survival time. Both fractions were able to restore the altered hematological and biochemical parameters. Hence, M. elengi stem bark may be a possible therapeutic candidate having cytotoxic and anti-tumor potential.

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Section: Introductionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

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“…In some Southeast Asian countries, extensive experience and abundant data on the treatment of cancers with traditional Chinese medicines (TCMs) have been documented (Zhang and Zhang 2008;Hu et al 2009). It is believed that formulae combined with multiple herbs under the guidance of theories of TCM could hit multiple targets and reduce the adverse effects Pedro et al 2007).…”

Section: Introductionmentioning

confidence: 99%

In vitro anti-proliferative effects of Zuojinwan on eight kinds of human cancer cell lines

et al. 2013

Cytotechnology

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Zuojinwan (ZJW), a famous Chinese medicinal formula, contains two medicinal herbs Coptis chinese Frach and Evodia rutaecarpa (Juss.) Benth in the ratio of 6: 1. The inhibitory effects of ZJW on eight kinds of human cancer cell lines including SMMC-7721, BEL-7402, BEL-7404, HepG2, A549, NCI-H446, NCI-H460 and HCT-116 cells were evaluated, and the possible mechanism was investigated. The growths of the eight kinds of cancer cells were inhibited by ZJW assessed through MTT assay. Flow cytometry assay revealed a sub-G1 peak with reduced DNA content was formed. The cell cycle was arrested in the G0/G1 phase in ZJW-treated SMMC-7721 and HepG2 cells, and in the S phase for NCI-H460 cells. Significant DNA damage was produced by ZJW assessed with single-cell gel electrophoresis assay. Morphological changes were also observed. Caspase-3 and -9 activities were increased following ZJW treatment. Western blot analysis showed that Bax and Bak protein levels were increased after ZJW treatment, while Bcl-2 and Bcl-xl protein levels were decreased. Our results suggest that ZJW has significant anti-cancer activities due to induction of mitochondria-dependent apoptosis pathway. Therefore, ZJW has the potential to be a novel chemotherapy drug to treat hepatoma, lung cancer and colon cancer by suppressing tumor growth.

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“…Flavonoid compounds found in a large number of plants were known to have anticancer activity (Erhart et al, 2005;Sonoda et al, 2004). In particular, several studies also showed that flavonoid compounds obtained from medicinal plants, such as apigenin, Astragalus complanatus flavonoids, and Lysimachia clethroides Duby flavonoids induced apoptosis in a human cancer cell line (Das et al, 2010;Hu et al, 2009;Liu et al, 2010). Chrysosplenium nudicaule is rich in flavonoids (Shi et al, 2011) and previous study showed flavonoid compound TTF isolated from C. nudicaule could inhibit the growth of the tumor cells from the blood system (Wang et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

A flavonoid compound fromChrysosplenium nudicauleinhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901

Luo

Yang

et al. 2015

Pharmaceutical Biology

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Context: Gastric cancer remains highly prevalent, but treatment options are limited. Natural products have proved to be a rich source of anticancer drugs. Chrysosplenium nudicaule Ledeb. (Saxifragaceae) is a perennial herb that grows in the highlands of China. It has been used as a traditional Chinese medicine to treat digestive diseases for hundreds of years. Recent studies revealed that this herb had anticancer activity, and the flavonoids were speculated to be the effective components. 6,7,3 0 -Trimethoxy-3,5,4 0 -trihydroxy flavone (TTF) and 5,4 0 -dihydroxy-3,6,3 0 trimethoxy-flavone-7-O-b-D-glucoside (DTFG) are flavonoid compounds isolated from Chrysosplenium nudicaule. Objective: This study examined the effect of TTF and DTFG on SGC-7901 human stomach cancer cell in vitro to determine the anticancer and induction of apoptosis properties of TTF. Materials and methods: The proliferation of cells treated with 32, 16, 8, 4, and 2 lg/mL of TTF or DTFG for 24, 48, and 72 h was assessed by the MTT assay. After being treated with TTF, the apoptosis of SGC-7901 cells was assessed by acridine orange staining, ultrastructure, electrophoresis of DNA fragmentation, and flow cytometry. Results: Results indicated that TTF inhibited the growth of cancer cells with an IC 50 value of 8.33 lg/mL after 72 h incubation. However, DTFG showed no inhibitory effect on the growth of the cancer cell. Further studies on TTF also confirmed that it was able to induce apoptosis of SGC-7901 cells at a concentration as low as 4 lg/mL. Discussion and conclusion: The apoptotic effect of TTF makes it a promising candidate for future chemotherapeutic application in treating stomach cancer.

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Induction of apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus

Cited by 86 publications

References 28 publications

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

In vitro anti-proliferative effects of Zuojinwan on eight kinds of human cancer cell lines

A flavonoid compound fromChrysosplenium nudicauleinhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901

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