Induction of Allergic Reactions in Guinea Pigs with Purified House Dust Mite Allergens

A new house dust mite–driven and mast cell–activated model of asthma in the guinea pig

“…To verify that HDM challenge induces allergic activation of mast cells, we quantified the levels of HDM‐specific immunoglobulins following the first challenge and found that HDM‐treated guinea pigs were indeed sensitized to the allergen as shown by increased levels of HDM‐specific IgG 1 and IgG 2 compared with PBS‐treated guinea pigs. The induction of both IgG subclasses in response to allergens, including dust mites, has previously been described in guinea pigs 18,22‐24 . Furthermore, it has been shown that both immunoglobulins can induce the ASM contraction, 22,25 also in line with our study.…”

Back to the future: re-establishing guinea pigin vivoasthma models

“…Accordingly, guinea pig elicits different patterns of allergic reactions. For instance, Yasue et al found that the allergic airway reaction provoked with rDer f 2 was higher than with whole mite extract, nevertheless, a further mite extract-challenge increased the airway resistance in a dose-dependent manner [109]. Interestingly, in the aforementioned study, only 50% of the guinea pigs showed an immediate airway reaction, whereas 87% revealed an immediate cutaneous reaction with erythema towards both allergens [109].…”

“…Moreover, the combination of intraperitoneal and subcutaneous injections together with aerosol boosters cause EAR and OVA-specific IgG 1 and IgE, besides AHR and eosinophilia, but no LAR [39,107]. Several other compounds have been used as allergens in guinea pig models, such as HDM extracts [108,109], purified proteins from mites [109], cockroach extracts [110][111][112], and trimellitic anhydride dust [113,114]. However, there have been very few models developed and AHR was not established.…”

“…For instance, Yasue et al found that the allergic airway reaction provoked with rDer f 2 was higher than with whole mite extract, nevertheless, a further mite extract-challenge increased the airway resistance in a dose-dependent manner [109]. Interestingly, in the aforementioned study, only 50% of the guinea pigs showed an immediate airway reaction, whereas 87% revealed an immediate cutaneous reaction with erythema towards both allergens [109]. Guinea pigs unresponsive to allergen challenges are frequently not included in the studies [26,109,123], even though these subjects might outline a similar phenotype to that observed in humans.…”

“…Interestingly, in the aforementioned study, only 50% of the guinea pigs showed an immediate airway reaction, whereas 87% revealed an immediate cutaneous reaction with erythema towards both allergens [109]. Guinea pigs unresponsive to allergen challenges are frequently not included in the studies [26,109,123], even though these subjects might outline a similar phenotype to that observed in humans. A recent study showed that guinea pigs with no allergen-induced bronchoconstriction do develop AHR and specific IgG 1 responses [107].…”