A new house dust mite–driven and mast cell–activated model of asthma in the guinea pig

Ramos‐Ramírez, Patricia; Noreby, Malin; Liu, Jielu; Ji, Jie; Abdillahi, Suado M; Olsson, Henric; Dahlén, Sven‐Erik; Nilsson, Gunnar; Adner, Mikael

doi:10.1111/cea.13713

Cited by 9 publications

(15 citation statements)

References 42 publications

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“…Briefly, the inflammation score was based on a five-point scoring system: normal = 0; a few cells = 1; one layer of cell ring = 2; two to four layers of cell ring = 3; >4 layers of cell ring = 4. The thickness of the airway wall and smooth muscle layer was calculated by airway wall thickness or smooth muscle layer thickness (μm) per length of basement membrane (mm) respectively ( Li et al, 2019 ; Ramos-Ramírez et al, 2020 ). The mucus score was according to a five-point scoring system, based on the percentage of goblet cells in the epithelium: no goblet cells = 0; <25% = 1; 25–50% = 2; 51–75% = 3; >75% = 4.…”

Section: Methodsmentioning

confidence: 99%

Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity

Jin

Hai-qin

et al. 2022

Front. Pharmacol.

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Metformin has been involved in modulating inflammatory state and inhibiting cell proliferation and angiogenesis. This study aimed to determine whether metformin alleviates airway inflammation and remodeling of experimental allergic asthma and elucidate the underlying mechanism. We sensitized and challenged mice with ovalbumin (OVA) to induce allergic asthma. During the challenge period, metformin was administered by intraperitoneal injection. By histopathological and immunohistochemical analyses, metformin-treated mice showed a significant alleviation in airway inflammation, and in the parameters of airway remodeling including goblet cell hyperplasia, collagen deposition and airway smooth muscle hypertrophy compared to those in the OVA-challenged mice. We also observed elevated levels of multiple cytokines (IL-4, IL-5, IL-13, TNF-α, TGF-β1 and MMP-9) in the bronchoalveolar lavage fluid, OVA-specific IgE in the serum and angiogenesis-related factors (VEGF, SDF-1 and CXCR4) in the plasma from asthmatic mice, while metformin reduced all these parameters. Additionally, the activity of 5′-adenosine monophosphate-activated protein kinase a (AMPKα) in the lungs from OVA-challenged mice was remarkably lower than control ones, while after metformin treatment, the ratio of p-AMPKα to AMPKα was upregulated and new blood vessels in the sub-epithelial area as evidenced by CD31 staining were effectively suppressed. These results indicate that metformin ameliorates airway inflammation and remodeling in an OVA-induced chronic asthmatic model and its protective role could be associated with the restoration of AMPKα activity and decreased asthma-related angiogenesis.

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Section: Methodsmentioning

confidence: 99%

Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity

Jin

Hai-qin

et al. 2022

Front. Pharmacol.

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“…Animals were placed on a heating pad and connected to the exiVent system 12 ( exiVent FX4, SCIREQ Inc, Montreal, Qc, Canada), where airway responses to methacholine (MCh) (1.56 mg/mL to 12.5 mg/mL) were assessed.…”

Section: Airway Responsiveness Testmentioning

confidence: 99%

Polyvinylalcohol-carbazate mitigates acute lung injury caused by hydrochloric acid

Dong

Liu

Quaranta

et al. 2022

Preprint

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Background Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), are important causes of morbidity and mortality in critically ill patients. Gastric contents aspiration is one of the most common causes of ALI/ARDS. To date, there are still no specific and effective pharmacological treatments for ALI/ARDS. Polyvinylalcohol-carbazate (PVAC), a polymer that has the ability to bind endogenous aldehydes, neutralize oxidative stress and inhibit inflammatory factors, may be a potential treatment for ALI/ARDS. Methods A hydrochloric acid (HCl) induced mouse model were employed to assess the effect of PVAC. The changes of lung mechanics, pulmonary edema, histology and immune cells, cytokines, and lipid mediators in bronchioalveolar lavage fluid (BALF) were investigated in HCl-challenged mice. Results In the HCl model, PVAC administration alleviated airway hyperresponsiveness and improved pulmonary edema and damage. In addition, it decreased the recruitment of neutrophils to the lung, and inhibited the increase of IL-6, TNF-α and leukotriene B4. Conclusions PVAC is a potential treatment for ALI/ARDS caused by inhalation of gastric acid.

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“…Other work covered the issue of product availability for venom immunotherapy in the UK, 115 epidemiology of allergic and respiratory disease 116‐119 , 120 , 121 occupational allergies, 122‐124 advances in allergy diagnostics 125‐133 , 134 , 135 , 136 , animal model work 137 138 , 139 , 140 , 141 , investigation of infant airway epithelium, 142 a possible association between tree pollen exposure and reduced lung function 143 and evaluations of allergy service delivery 144‐147 …”

Section: Other Areas Of Interestmentioning

confidence: 99%

Developments in the field of allergy in 2020 through the eyes of Clinical and Experimental Allergy

Boyle

Shamji

2021

Clin Experimental Allergy

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A new house dust mite–driven and mast cell–activated model of asthma in the guinea pig

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 9 publications

References 42 publications

Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity

Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity

Polyvinylalcohol-carbazate mitigates acute lung injury caused by hydrochloric acid

Developments in the field of allergy in 2020 through the eyes of Clinical and Experimental Allergy

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