Induction effect of coadministration of soybean isoflavones and sodium nitrite on DNA damage in mouse stomach

Toyoizumi, Tomoyasu; Sekiguchi, Hirotaka; Takabayashi, Fumiyo; Deguchi, Yuya; Masuda, S.; Kinae, Naohide

doi:10.1016/j.fct.2010.06.015

Cited by 14 publications

(12 citation statements)

References 47 publications

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“…Thus, this study has demonstrated that non-react isoflavones at high concentrations have the ability to clear free radicals in stomach. In studies where daidzein, genistein, and NaNO 2 were given individually, no lymphocytic infiltration was induced [14]. Similarly, in our study the inflammatory response was less prominentin rest of all the groups.…”

Section: Discussionsupporting

confidence: 69%

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Changes in Ki-67 and Bcl-2 Expression in Rat Gastric Mucosa Secondary to Soybean Feeding: An Assessment of Proliferative and Apoptotic Activity of Flavonoids.

Sivrikoz¹,

Keçeci

Sanal³

et al. 2018

JNB

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Background: Gastric cancer is a common malignancy and nutritional factors are believed to play an important role in its development. Isoflavones in soybean products are an important nutritional source, especially in some Asian Countries, having protective role against development of gastric cancer.In our study, we have prepared soybean extracts via different methodologies and assessed their apoptotic role and proliferation index in gastric mucosa of the rodents.Methodology: We have fed 2 months old 64 rats for a period of 1 month with soybean extracts prepared by 3different methodologies and in 6 different concentrations. Subsequently, the rodents were subjected to euthanasia and their gastric mucosal linings were processed accordingly for H & E, Bcl-2, and Ki 67 staining. Thereafter, the specimens were evaluated under light microscopy.Results: In all the experimental groups fed with soybean extracts, weak staining was observed with Bcl-2, moresignificantly in the group of rodents fed with 100 mg/kg and 200 mg/kg ethanol extract as well as in the group fed with high ethyl acetate extract (p<0.05). There was no difference between the groups with respect to Ki 67 staining and inflammation rate (p>0.05).Conclusions: We have discovered that soybean extracts and specifically genistin and daidzein induces apoptosis at high concentrations. This observation makes us believe that soybeans have a protective role in the development of gastric cancer.

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Section: Discussionsupporting

confidence: 69%

“…More importantly, endogenous nitrate production is increased in the stomach during helicobacter pylori infection. This in turndecreases the gastric vitamin C concentration and leads to overproduction of N-nitroso compounds [14].…”

Section: Discussionmentioning

confidence: 99%

Changes in Ki-67 and Bcl-2 Expression in Rat Gastric Mucosa Secondary to Soybean Feeding: An Assessment of Proliferative and Apoptotic Activity of Flavonoids.

Sivrikoz¹,

Keçeci

Sanal³

et al. 2018

JNB

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“…Briefly, ten μl of 8 × 10 3 cells/mL cell suspension was mixed with 90 μl of 0.5% low melting point agarose and the mixture was spread on the MAS coated glass slide. After immersion in lysing solution, slides were incubated for 30 min at 37 °C with or without formadidopyrimidine-DNA glycosylase (FPG) protein (Sigma-Aldrich, St Louis, MO) in order to confirm oxidative DNA damage [12]. Thereafter, each slide was then placed in alkaline electrophoresis buffer to allow for DNA unwinding.…”

Section: Methodsmentioning

confidence: 99%

Effect of physicochemical character differences on the genotoxic potency of kaolin

et al. 2017

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BackgroundKaolin is white clay mineral with the chemical composition Al2Si2O5(OH)4, and many varieties of kaolins having different crystal structures are utilized in industrial, cosmetic and medical fields. To evaluate the effect of physicochemical character differences on the genotoxicity of kaolin, two types of kaolin, kaolin-S with smooth, sphere-shaped crystals, and kaolin-P with clusters of thin pseudohexagonal plates, were used in the study.ResultsICR mice were intratracheally instilled with the kaolins (0.05 and 0.2 mg/mouse), and comet assay was performed on their lungs. Both kaolins showed DNA damage in the lungs of the mice, however the DNA damaging potency was much higher with kaolin-P than that with kaolin-S.In order to clarify the mechanisms for the different genotoxic potency, we examined the incorporation rate and ROS generation of these two types of kaolin in alveolar epithelial A549 and macrophage-like RAW264 cells, using flow cytometric (FCM) analysis. Kaolin-P showed a higher incorporation rate into the mammalian cells and ROS generation than that of kaolin-S. Especially, RAW264 cells aggressively incorporated kaolins, and generated ROS, whereas almost no ROS generation was observed in A549 cells. In addition, inflammatory cytokines were quantified, using the ELISA method, to understand further genotoxic potency differences of kaolins. Concentrations of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the media were increased by exposure to both kaolins, but in the case of kaolin-P, these inflammatory cytokines were significantly elevated. Based on these findings, differences of genotoxic potency may contribute to incorporation rates into immune cells. Furthermore, it is likely that immune cells and epithelial cells might closely interact with each other for the appearance of genotoxocity in vivo. In order to clarify the interaction between epithelial and immune cells, A549 and RAW264 were co-cultured and RAW264 cells only were exposed to kaolins, then subsequently A549 was applied to FCM analysis and comet assay. DNA damage observed in the A549 cells markedly increased in the presence of kaolin-exposed RAW264 cells compared to the single culture.ConclusionFrom these observations, it is suggested that mechanisms of kaolin genotoxicity against epithelial cells are through the activation of macrophage cells. Therefore, it is thought that interactions between epithelial and immune cells would be very important for evaluation of the genotoxicity of fine particulate matter. We also showed here that co-culture models of epithelial and immune cells could be used as suitable models for evaluation of lung genotoxicity of fine particulate matter, including nanomaterials, as in vivo mimicking systems.

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“…[27] In addition, nitrite has a potential clinical risk. [11,12,23] Importantly, it was observed that H 2 -induced nitrate production and prevented nitrite accumulation in tomato fruit was in a time-dependent fashion (Figure 1d-e). With eggplant, lettuce, daylily flower, spinach, apple and carrot as experimental materials, further time-course experiments showed that H 2 -prevented nitrite accumulation in these plant materials during storage, demonstrating that H 2 was a universal preservation agent (Supplementary Figure S4).…”

Section: Resultsmentioning

confidence: 81%

“…[8,9] Besides the signaling role of NO in the human body, these RNS can also react with body tissues and trigger enzyme inactivation, DNA lesions, lipid peroxidation, damage different organs, and can be considered as one of the most important human dietary carcinogenic factors. [10][11][12] Nitrite, in particular, can easily react with amides and amines to produce carcinogenic N-nitroso compounds. [13][14][15][16][17] According to some epidemiological and toxicological studies, N-nitroso compounds may potentially increase the risk of esophageal and stomach cancer.…”

Section: Introductionmentioning

confidence: 99%

Nitrite accumulation during storage of tomato fruit as prevented by hydrogen gas

Zhang

Zhao

Cheng

et al. 2019

International Journal of Food Properties

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The adverse effects of intake nitrite upon human health are well known. However, eating fruits and vegetables is one of the main pathways to absorb nitrite because of nitrogen assimilation in plants. This study demonstrated that during storage of tomato fruit, the production of endogenous hydrogen (H 2 ) was decreased, in parallel with nitrite accumulation and the senescence rate. Furthermore, exogenously applied H 2 could delay the decreased fruit H 2 production and senescence, but importantly, nitrite accumulation was blocked. Consistently, the activities and transcripts of nitrate reductase (NR; catalyzing the synthesis of nitrite) and nitrite reductase (NiR; responsible for the reduction of nitrite to ammonium), were either inhibited or increased, respectively, by 0.585 mM H 2 . Decreased or increased nitrite synthesis was observed when tungstate (an inhibitor of NR) or 2,6-dichloroindophenol sodium salt (a putative inhibitor of H 2 synthesis) were applied separately. Time-course analysis revealed that the decrease in vitamin C, a well-known nitrite scavenger, was blocked by H 2 . Overall, this study strongly revealed that nitrite accumulation during storage of tomato fruit was prevented by H 2 . This study describes potential applications for H 2 in agriculture and food industry, especially in the preservation of fruit and vegetable products.

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Induction effect of coadministration of soybean isoflavones and sodium nitrite on DNA damage in mouse stomach

Cited by 14 publications

References 47 publications

Changes in Ki-67 and Bcl-2 Expression in Rat Gastric Mucosa Secondary to Soybean Feeding: An Assessment of Proliferative and Apoptotic Activity of Flavonoids.

Changes in Ki-67 and Bcl-2 Expression in Rat Gastric Mucosa Secondary to Soybean Feeding: An Assessment of Proliferative and Apoptotic Activity of Flavonoids.

Effect of physicochemical character differences on the genotoxic potency of kaolin

Nitrite accumulation during storage of tomato fruit as prevented by hydrogen gas

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