Induction, distribution and modulation of upper airway allergic inflammation in mice

Abstract

The mouse upper airway, specifically in areas containing respiratory epithelium, is a target for OVA-induced allergic inflammation. This selective infiltration of respiratory, but not olfactory, epithelium is, in part, dependent upon IL-5. This model is useful for further dissection of the inflammatory response with genetic manipulations and targeted immunological approaches.

Systemic Administration of Olygodeoxynucleotides with CpG Motifs at Priming Phase Reduces Local Th2 Response and Late Allergic Rhinitis in BALB/c Mice

“…On the other hand, thickening of basement membrane, hyperplasia of nasal glands in inflamed nasal areas and inflammation in the areas of olfactory epithelium in human patients with allergic rhinitis [46] were not found in the present mouse model. Although, we have no explanation for the discrepancy of these histologic changes between human and animal, it must be a property of the species, including anatomical and physiological differences [38].…”

“…Figure 2a shows the right side of transverse section. Areas of rectangles (S; lower portion of the nasal septum, F; the floor, L; the lower lateral walls) of respiratory epithelium were examined, since it has been reported that allergic inflammation was mainly localized in respiratory epithelium of these parts in a murine allergic rhinitis model [38,39]. Paraffin sections (4 mm thickness) were stained with hematoxylin and eosin, alcian blue (AB) and phosphotungstic acid hematoxylin.…”

T‐helper 1 and T‐helper 2 adjuvants induce distinct differences in the magnitude, quality and kinetics of the early inflammatory response at the site of injection

“…This is in agreement with the work of Walls 49 and with studies of aluminium-induced experimental allergy. 50 Furthermore, IL-5 was the only cytokine or chemokine found to be selectively expressed early after injection of Al(OH) 3 . IL-5 is strongly involved in eosinophilopoiesis and has a known synergistic effect on the ability of eotaxins to attract eosinophils.…”

Development of an Animal Model for Wound Healing in Chronic Rhinosinusitis

“…The skulls were then sectioned coronally into the following 3 regions for paraffin embedding: (A) caudal to the incisor teeth, (B) halfway between A and C, and (C) anterior margin of the orbit. 4 Sections were approximately 2 to 3 mm thick and slides were prepared 0.5-µm thick from the face of each section. Hematoxylin-eosin (HE) was used to characterize the inflammatory infiltrate at standardized anatomic locations according to nasal diagrams.…”