Induction control of purine catabolism in Schizosaccharomyces pombe

Fluri, R.; Kinghorn, James R.

doi:10.1007/bf00381170

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…In several fungi it has been characterized as a strong inducer of enzymes of purine catabolism, namely, xanthine oxidase and urate oxidase in Aspergillus nidulans (37), Neurospora crassa (27), and Schizosaccharomyces spp. (13), which is consistent with the ability of saprophytes to use uric acid as a substrate, e.g., Histoplasma (23) and Hyphomyces (22) spp. It is now well established that the expression of several structural genes involved in purine uptake and catabolism is subject to uric acid induction and mediated by the product of the positive regulatory gene uaY (9,10,16), including posttranslational modifications of the degrading enzymes (1).…”

Section: Discussionsupporting

confidence: 76%

Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus

Helbig¹,

Steighardt²,

Roos³

2002

Appl Environ Microbiol

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On searching for endogenous, low-molecular-weight effectors of benzodiazepine alkaloid biosynthesis in Penicillium cyclopium uric acid was isolated from ethanolic or autoclaved mycelial extracts of this fungus. The isolation was based on a three-step high-pressure liquid chromatography procedure guided by a microplate bioassay, and uric acid was identified by mass spectrometry and the uricase reaction. Conidiospore suspensions that were treated with this compound during the early phase of outgrowth developed emerged cultures with an enhanced rate of alkaloid production. Uric acid treatment did not increase the in vitro measurable activity of the rate-limiting biosynthetic enzyme, cyclopeptine synthetase. However, these cultures displayed a reduced rate of uptake of the alkaloid precursor L-phenylalanine into the vacuoles of the hyphal cells as assayed in situ. It is suggested that the depressed capacity of vacuolar uptake caused by the contact of outgrowing spores with uric acid liberated from hyphal cells results in an enhanced availability of the precursor L-phenylalanine in the cytoplasm and thus accounts at least in part for the increase in alkaloid production.

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Section: Discussionsupporting

confidence: 76%

Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus

Helbig¹,

Steighardt²,

Roos³

2002

Appl Environ Microbiol

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“…Examination of the various classes of mutants that do not utilize purines has permitted the identification of the true inducers of the pathway. These studies suggest that control is brought about by sequential induction of the enzymes by various intermediates of the pathway (Fluri & Kinghorn, 1985). Uricase is induced by uric acid, allantoinase and allantoicase by allantoic acid and ureidoglycollase by ureidoglycollic acid.…”

Section: Introductionmentioning

confidence: 92%

“…It is clear that induction of uricase was affected by a112 mutations. This was particularly marked with hypoxanthine, which has to be converted to uric acid, the true inducer, before induction can occur (Fluri & Kinghorn, 1985). a112 mutants show less reduction of activity with uric acid itself as the inducer.…”

Section: Growth Responses Of All2 Strainsmentioning

confidence: 99%

“…Uricase, allantoinase, allantoicase and ureidoglycollase are highly inducible and this induction t Correspondence should be sent to the St Andrews address. is brought about by purines (Fluri & Kinghorn, 1985). It is not yet known, except for uricase, whether control is at the level of transcription or translation or both.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro translation studies show that, in the case of uricase, induction control is at the level of transcription (R. Fluri, unpublished work). In contrast, the regulation of urease, the final step in the pathway, is constitutive (Fluri & Kinghorn, 1985). Examination of the various classes of mutants that do not utilize purines has permitted the identification of the true inducers of the pathway.…”

Section: Introductionmentioning

confidence: 99%

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The all2 Gene is Required for the Induction of the Purine Deamination Pathway in Schizosaccharomyces pombe

Fluri¹,

Kinghorn²

1985

Microbiology

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Five mutants were isolated at the all2 gene on the basis of their inability to utilize hypoxanthine as a sole source of nitrogen. These mutants failed to utilize the purines adenine, hypoxanthine, xanthine, uric acid, allantoin and allantoic acid, although they could utilize urea and ammonium. The all2 mutants appeared to be defective in purine induction of uricase, allantoinase, allantoicase and ureidoglycollase activities but retained wild-type activity of the constitutively synthesized urease. The all2 mutations were recessive.

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Degradation of organic nitrogen compounds by yeasts

Large

1986

Yeast

118

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Induction control of purine catabolism in Schizosaccharomyces pombe

Cited by 11 publications

References 12 publications

Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus

Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus

The all2 Gene is Required for the Induction of the Purine Deamination Pathway in Schizosaccharomyces pombe

Degradation of organic nitrogen compounds by yeasts

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