Induction and Regeneration of Autoplasts from
            <i>Clostridium thermohydrosulfuricum</i>
            JW102 and
            <i>Thermoanaerobacter ethanolicus</i>
            JW200

Peteranderl, Ralph; Canganella, Francesco; Holzenburg, Andreas; Wiegel, Juergen

doi:10.1128/aem.59.10.3498-3501.1993

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…strain JW/SL‐YS485 is resistant to 100 μg ml −1 ampicillin, 50 μg ml −1 chloramphenicol, but sensitive to kanamycin with a MIC of 25 μg ml −1 . To transform Thermoanaerobacterium , the cells were converted to autoplasts as previously described [9] and prepared for electroporation as described in (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance

Mai

Lorenz

Wiegel

2006

FEMS Microbiology Letters

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The industrial application of thermophilic (eu)bacteria is hampered by the lack of genetic systems for these bacteria. We report here the first unequivocal transformation of a Gram‐positive, thermophilic, anaerobic microorganism, Thermoanaerobacterium, with the kanamycin resistance‐mediating plasmid pIKM1. The construct pIKM1 is based on the Escherichia coli–Clostridium acetobutylicum shuttle vector pIMP1 and contains the thermostable kanamycin cassette from S. faecalis plasmid pKD102. Using electrotransformation, plasmid pIKM1 mediated kanamycin resistance in Thermoanaerobacterium sp. strain JW/SL‐YS485 up to 400 μg ml−1 at 48°C and 200 μg ml−1 at 60°C.

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Section: Resultsmentioning

confidence: 99%

Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance

Mai

Lorenz

Wiegel

2006

FEMS Microbiology Letters

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Thermoanaerobacter

Onyenwoke

Wiegel

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Ther.mo.an.ae.ro.bac'ter. Gr. adj. thermos hot; Gr. pref. an not; Gr. n. aer air; M.L. bacter masc. equivalent of Gr. neut. dim. n. bakterion rod staff; N.L. masc. n. Thermoanaerobacter rod which grows in the absence of air at elevated temperatures. Firmicutes / “Clostridia” / Thermoanaerobacterales / Thermoanaerobacteraceae / Thermoanaerobacter All species are obligately anaerobic thermophiles and have a Gram‐positive cell wall , i.e., although the Gram‐stain reaction varies among the individual species and even within strains from the same species, they have a Gram‐positive cell‐wall structure. Most species exhibit a sluggish motility and a peritrichous arrangement of 2–6 flagella (retarded peritrichous flagellation). Cells are rod shaped and occur in various arrangements that vary by species. Many of the Thermoanaerobacter species form pleomorphic cells during the late exponential and stationary growth phases assembling through nonsymmetric cell division chains of alternating rod‐shaped and coccoid cells. Other types of pleomorphic rods are formed, presumably through weakening of the cell wall by lysozyme‐like enzymes that are the remnants of the sporulation process ( Figure 1). Growth temperature optima range from 55–75 °C, with growth ranges of 35–78 ° C and a temperature span over 35 ° C . The pH for growth ranges from pH 25C 4 . 0–9 . 9 , with a pH optimum of 5.8–8.5. Several species also exhibit a wide pH optimum of about 3 units without a sharp peak. All species are able to grow organoheterotrophically using various fermentation pathways including the homoacetogenic or Wood–Ljungdahl pathway, or using inorganic electron acceptors (e.g., Thermoanaerobacter sulfuriphilus using S 0 as an electron acceptor and lactate as an electron donor and carbon source). Some Thermoanaerobacter strains are also able to grow chemolithoheterotrophically by coupling H 2 oxidation to growth (Fardeau et al., 1994). Furthermore, others are facultative chemolithoautotrophs , growing with H 2 + CO 2 (such as Thermoanaerobacter kivui ) or Fe(III) + H 2 + CO 2 (such as Thermoanaerobacter siderophilus ). Generally, typical fermentation products from hexoses are ethanol, acetate, lactate, H 2 , and CO 2 . DNA G + C content ( mol %): 30–39. Type species : Thermoanaerobacter ethanolicus Wiegel and Ljungdahl 1982, 384 VP (Effective publication: Wiegel and Ljungdahl 1981, 348.).

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Thermoanaerobacterium

Onyenwoke¹,

Wiegel²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Ther.mo.an.ae.ro.bac'te.ri.um. Gr. n. thermos hot, Gr. pref. an not, Gr. n. aer air, Gr. n. bakterion a small rod, N.L. neut. n. Thermoanaerobacterium a rod which grows in the absence of air at high temperatures. Firmicutes / “Clostridia” / Thermoanaerobacterales / incertae Sedis ‐ Family III / Thermoanaerobacterium Extreme thermophiles with growth temperature optima between 55 and 70 °C. Cells have Gram‐positive cell‐wall structure , but many strains are Gram‐stain‐negative . Cells are rod‐shaped and motile by peritrichously inserted flagella, except for Thermoanaerobacterium aciditolerans , which has a single, polar flagellum. Endospores are present in some species . Hexagonal S‐layer typically present. However, it may be difficult to visualize because of extracellular hydrolytic enzymes attached to the cells. Obligate anaerobes and catalase‐negative. DNA G + C content ( mol %): 29–46. Type species : Thermoanaerobacterium thermosulfurigenes (Schink and Zeikus 1983a) Lee, Jain, Lee, Lowe and Zeikus 1993a, 48 VP ( Clostridium thermosulfurogenes Schink and Zeikus 1983a, 1156).

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Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200

Cited by 6 publications

References 18 publications

Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance

Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance

Thermoanaerobacter

Thermoanaerobacterium

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