<i>Thermoanaerobacterium</i>

Onyenwoke, Rob U.; Wiegel, Juergen

doi:10.1002/9781118960608.gbm00755

Cited by 6 publications

(5 citation statements)

References 129 publications

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“…In the genome of strain 3443-3Ac T the genes that we predict to encode HDCR subunits are not located in a single gene cluster. Genome analysis revealed the presence et al (1998), Romano et al (2010), Shang et al (2013), Onyenwoke and Wiegel (2015), and Lopez et al (2017)], Thermoanaerobacter [data from Wagner et al (2008), Tomas et al (2013), and Onyenwoke and Wiegel (2015)], and Caldanaeobacter [data from Kim et al (2001), Kozina et al (2010), and Fardeau et al (2015)].…”

Section: Genomic Determinants Of Acetogenesis In Strain 3443-3ac Tmentioning

confidence: 99%

Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium

et al. 2023

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One of the important current issues of bioenergetics is the establishment of the thermodynamic limits of life. There is still no final understanding of what is the minimum value of the energy yield of a reaction that is sufficient to be used by an organism (the so-called “biological quantum of energy”). A reasonable model for determination of the minimal energy yield would be microorganisms capable of living on low-energy substrates, such as acetogenic prokaryotes. The most prominent metabolic feature of acetogens is autotrophic growth with molecular hydrogen and carbon dioxide as the substrates, which is hardly competitive in environments. Most probably, that is why only facultative autotrophic acetogens have been known so far. Here, we describe the first obligately autotrophic acetogenic bacterium Aceticella autotrophica gen. nov., sp. nov., strain 3443-3AcT. Phylogenetically, the new genus falls into a monophyletic group of heterotrophic bacteria of the genera Thermoanaerobacterium, Thermoanaerobacter, and Caldanaerobacter (hereinafter referred to as TTC group), where the sole acetogenic representative has so far been the facultatively autotrophic Thermoanaerobacter kivui. A. autotrophica and T. kivui both are acetogens employing energy-converting hydrogenase (Ech-acetogens) that are likely to have inherited the acetogenesis capacity vertically from common ancestor. However, their acetogenic machineries have undergone different adjustments by gene replacements due to horizontal gene transfers from different donors. Obligate autotrophy of A. autotrophica is associated with the lack of many sugar transport systems and carbohydrate catabolism enzymes that are present in other TTC group representatives, including T. kivui.

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Section: Genomic Determinants Of Acetogenesis In Strain 3443-3ac Tmentioning

confidence: 99%

Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium

et al. 2023

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“…T. thermosaccharolyticum (previously known as Clostridium thermosaccharolyticum ) is a similar hemicellulose-fermenting species with a pH optimum of 7.0 or higher 10 , 11 , and has long been recognized as a potential species of industrial utility. In 1981, strain HG-4 was grown with C. thermocellum to produce 25.3 g/L ethanol from model substrates and 9.7 g/L ethanol from corn stover 12 .…”

Section: Introductionmentioning

confidence: 99%

“…More than 1 billion pounds of 1,2-propanediol are produced annually in the United States 15 , but fermentation from renewable feedstocks is not yet cost-competitive with its chemical synthesis 16 . Many bacteria, including T. thermosaccharolyticum, produce 1,2-propanediol from the sugar rhamnose 11 , 16 . Efforts were made to engineer 1,2-propanediol production from glucose in E. coli , with a maximum titer of 4.5 g/L 17 .…”

Section: Introductionmentioning

confidence: 99%

Growth-uncoupled propanediol production in a Thermoanaerobacterium thermosaccharolyticum strain engineered for high ethanol yield

Herring

Ajie

Lynd

2023

Sci Rep

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Cocultures of engineered thermophilic bacteria can ferment lignocellulose without costly pretreatment or added enzymes, an ability that can be exploited for low cost biofuel production from renewable feedstocks. The hemicellulose-fermenting species Thermoanaerobacterium thermosaccharolyticum was engineered for high ethanol yield, but we found that the strains switched from growth-coupled production of ethanol to growth uncoupled production of acetate and 1,2-propanediol upon growth cessation, producing up to 6.7 g/L 1,2-propanediol from 60 g/L cellobiose. The unique capability of this species to make 1,2-propanediol from sugars was described decades ago, but the genes responsible were not identified. Here we deleted genes encoding methylglyoxal reductase, methylglyoxal synthase and glycerol dehydrogenase. Deletion of the latter two genes eliminated propanediol production. To understand how carbon flux is redirected in this species, we hypothesized that high ATP levels during growth cessation downregulate the activity of alcohol and aldehyde dehydrogenase activities. Measurements with cell free extracts show approximately twofold and tenfold inhibition of these activities by 10 mM ATP, supporting the hypothesized mechanism of metabolic redirection. This result may have implications for efforts to direct and maximize flux through alcohol dehydrogenase in other species.

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“…Therefore, it has ferromagnetism and high biocompatibility, and is expected to be used as a magnetic resonance imaging (MRI) contrasting agent and for target drug delivery, for example. [16][17][18] The general methods of MNP synthesis are thermal decomposition 19) and coprecipitation. 20) Thermal decomposition is a method in which iron organic precursors such as Fe(CO) 5 and Fe(C 5 H 7 O 2 ) 3 are decomposed in deoxygenated high-temperature solvents including a reducing agent and a stabilizer.…”

Section: Introductionmentioning

confidence: 99%

Chemical reaction process for magnetite nanoparticle synthesis by atmospheric-pressure DC glow-discharge electrolysis

Yamazaki

Shirai

Nakagawa

et al. 2018

Jpn. J. Appl. Phys.

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We investigated the reaction process in magnetite nanoparticle (MNP) synthesis by glow-discharge electrolysis in atmospheric air combined with iron electrolysis using NaCl aqueous solution as electrolyte. The iron electrolysis supplies Fe2+ in the solution, and electrons from the glow discharge induce liquid-phase reactions. We found experimentally that the concentration of dissolved oxygen (DO) in the solution is a key parameter in MNP synthesis. The excess oxidation of ferrous iron species at a high DO concentration causes the generation of hematite nanoparticles, while MNPs are mainly synthesized at a low DO concentration. Simple rate equations were solved to investigate the liquid-phase reaction process. The calculated results showed that the DO concentration reasonably changed the ratio between ferrous and ferric iron species, which will be important for the chemical composition of synthesized nanoparticles. The solution pH, which is locally increased by the glow discharge, affects the liquid-phase reaction process, especially through the hydroxylation of ferrous iron species.

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Thermoanaerobacterium

Cited by 6 publications

References 129 publications

Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium

Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium

Growth-uncoupled propanediol production in a Thermoanaerobacterium thermosaccharolyticum strain engineered for high ethanol yield

Chemical reaction process for magnetite nanoparticle synthesis by atmospheric-pressure DC glow-discharge electrolysis

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