Induction and preliminary characterization of intracellular $beta;-glucosidases from a cellulolytic Streptomyces strain

PEREZPONS, J

doi:10.1016/0378-1097(95)00095-m

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“…This finding was consistent with the work of Pérezpons who reported that β-glucosidase activity of Streptomyces sp. QM-B814 was inhibited in the presence of glucose [ 50 ]. The results suggested that, at sufficiently high glucose concentrations, the functional microbial community was altered and contributes to maintaining the operation of the enzyme despite the high glucose content.…”

Section: Discussionmentioning

confidence: 99%

The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting

Zang

Liu

Fan

et al. 2018

Biotechnol Biofuels

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BackgroundCompost habitats sustain a vast ensemble of microbes that engender the degradation of cellulose, which is an important part of global carbon cycle. β-Glucosidase is the rate-limiting enzyme of degradation of cellulose. Thus, analysis of regulation of β-glucosidase gene expression in composting is beneficial to a better understanding of cellulose degradation mechanism. Genetic diversity and expression of β-glucosidase-producing microbial communities, and relationships of cellulose degradation, metabolic products and the relative enzyme activity during natural composting and inoculated composting were evaluated.ResultsCompared with natural composting, adding inoculation agent effectively improved the degradation of cellulose, and maintained high level of the carboxymethyl cellulose (CMCase) and β-glucosidase activities in thermophilic phase. Gene expression analysis showed that glycoside hydrolase family 1 (GH1) family of β-glucosidase genes contributed more to β-glucosidase activity in the later thermophilic phase in inoculated compost. In the cooling phase of natural compost, glycoside hydrolase family 3 (GH3) family of β-glucosidase genes contributed more to β-glucosidase activity. Intracellular β-glucosidase activity played a crucial role in the regulation of β-glucosidase gene expression, and upregulation or downregulation was also determined by extracellular concentration of glucose. At sufficiently high glucose concentrations, the functional microbial community in compost was altered, which may contribute to maintaining β-glucosidase activity despite the high glucose content.ConclusionThis research provides an ecological functional map of microorganisms involved in carbon metabolism in cattle manure–rice straw composting. The performance of the functional microbial groups in the two composting treatments is different, which is related to the cellulase activity and cellulose degradation, respectively.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1045-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%