Induction and micropropagation potential of sugar beet haploids

Abstract

The aim of research was obtaining sugar beet haploids via gyno-genesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cyto-kinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each sub-cultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes

Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)

“…To induce androgenesis in anther cultures were used: B 5 media (Gamborg et al, 1968) with different sucrose content (140 g·L -1 and 100 g·L , supplemented with 0.1 mg·L -1 2,4-D, 0.2 mg·L -1 BAP and 0.5 mg·L -1 IAA, both set with 6.5 g·L -1 agar, pH 5.8. Anther cultures were kept at a temperature of +35 °C for 24 h and then were carried out in the darkness at +24 °C (Nagl et al, 2004). To obtain a larger number of androgenic embryos in the following experiment anthers of "Czerwona Kula" variety were laid out on medium.…”

Sugar Beet Micropropagation

“…Haploids are obtained by ovule culture from unpollinated ovules via process called gynogenesis (10, 52) Percent of haploid induction varies from 0% up to 35%, which strictly depends from genotype and its interaction with amount of citokinins in nutrient medium (51). Obtained haploids can also be multiplied via axillary bud induction and although all genotypes have ability to multiply, after prolonged time in micropropagation there can be detected differences in micropropagation rates (35). One of the very efficient methods for sugar beet dihapliod induction is short-term micropropagation of haploids on medium with colchicine and later cytological selection of autodiploids (25,30).…”

Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro

“…The obtained DH lines are used in breeding and seed production processes to create sugar beet hybrids in many European firms [33]. The development of this technique is being carried out in Germany [38], Sweden [39], Russia [33], Belarus [40], Serbia [41], Denmark [42], Turkey [43][44][45], Poland [46], and Iran [47]. However, the efficiency of gynogenesis induction in sugar beet remains negligible in most scientific studies (ranging from 1% to 15%), and the efficiency of regenerant yield is 40% [39].…”