Induction and Activation of Adaptive Immune Populations During Acute and Chronic Phases of a Murine Model of Experimental Colitis

Hall, Lindsay J.; Faivre, Émilie; Quinlan, Aoife; Shanahan, Fergus; Nally, Ken; Melgar, Silvia

doi:10.1007/s10620-010-1240-3

Cited by 92 publications

(85 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results correlate with recent studies indicating that CD4 ϩ T cells become activated in the periphery in the DSS-induced colitis model and home to the colon during the first 3-7 days of DSS administration (55,56). Furthermore, Hall et al (43) showed that adaptive immune populations such as CD3 ϩ and B cells are also activated in the late acute phase of DSS-induced colitis. We show that AS101 treatment, starting either at or after disease onset, prevents the …”

Section: Figure 7 As101 Prevents the Migration Of ␣4␤7supporting

confidence: 92%

“…Because the severity of clinical symptoms in our protocol (e.g. intestinal bleeding) started to increase 2 days after DSS administration, together with the fact that the onset of the inflammatory and apoptotic process in the colon of DSStreated mice starts 24 h after DSS administration (43,44), we decided to check the effect of AS101 compound given 2 days after DSS administration.…”

Section: As101 Reduces Clinical Symptoms Of Dss-induced Murinementioning

confidence: 99%

See 1 more Smart Citation

Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis

Halpert

Eitan

Voronov

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: AS101, a novel small tellurium compound, is a potent anti-inflammatory/anti-apoptotic immunomodulator. Results: AS101 prevents leukocyte migration and protects the mucosal barrier in murine colitis. Conclusion: AS101 exerts multifunctional anti-inflammatory/anti-apoptotic activity in experimental inflammatory bowel diseases. Significance: This is the first report showing significant effect of tellurium-based compound for the treatment of inflammatory bowel diseases.

show abstract

Section: Figure 7 As101 Prevents the Migration Of ␣4␤7supporting

confidence: 92%

Section: As101 Reduces Clinical Symptoms Of Dss-induced Murinementioning

confidence: 99%

Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis

Halpert

Eitan

Voronov

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Male mice, C57BL/6OlaHsD (Harlan, UK) aged 10-12 weeks, were housed in groups of five per cage on a 12 h light/dark cycle. Mice were given 3% DSS (45 kDa; TdB Consultancy, Uppsala, Sweden, prepared fresh daily) in their drinking water for 4 days as described previously [47]. In the control group animals received water.…”

Section: Animals and Dextran Sodium Sulfate (Dss)-induced Colitismentioning

confidence: 99%

A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression

Zhdanov

Okkelman

Collins

et al. 2015

Biochimica et Biophysica Acta (BBA) - Bioenergetics

Self Cite

View full text Add to dashboard Cite

Abnormal accumulation of oncometabolite fumarate and succinate is associated with inhibition of mitochondrial function and carcinogenesis. By competing with α-ketoglutarate, oncometabolites also activate hypoxia inducible factors (HIFs), which makes oncometabolite mimetics broadly utilised in hypoxia research. We found that dimethyloxalylglycine (DMOG), a synthetic analogue of α-ketoglutarate, commonly used to induce HIF signalling, inhibits O2 consumption in cancer cell lines HCT116 and PC12, well before activation of HIF pathways. A rapid suppression of cellular respiration was accompanied by a decrease in histone H4 lysine 16 acetylation and not abolished by double knockdown of HIF-1α and HIF-2α. In agreement with this, production of NADH and state 3 respiration in isolated mitochondria were down-regulated by the de-esterified DMOG derivative, N-oxalylglycine. Exploring the roles of DMOG as a putative inhibitor of glutamine/α-ketoglutarate metabolic axis, we found that the observed suppression of OxPhos and compensatory activation of glycolytic ATP flux make cancer cells vulnerable to combined treatment with DMOG and inhibitors of glycolysis. On the other hand, DMOG treatment impairs deep cell deoxygenation in 3D tissue culture models, demonstrating a potential to relieve functional stress imposed by deep hypoxia on tumour, ischemic or inflamed tissues. Indeed, using a murine model of colitis, we found that O2 availability in the inflamed colon tissue rapidly increased after application of DMOG, which could contribute to the known therapeutic effect of this compound. Overall, our results provide new insights into the relationship between mitochondrial function, O2 availability, metabolic reprogramming and associated diseases.

show abstract

“…They showed that adaptive immune responses are induced during colitis (21). Thus, we asked whether the local GALT immune cells are recruited and activated during mild (1% DSS treated) or severe colitis (3.5% DSS treated).…”

Section: Resultsmentioning

confidence: 99%

Local immune compartments are related to the severity of dextran sodium sulphate induced colitis

Wang¹,

Jiang²,

Liu³

et al. 2014

BST

View full text Add to dashboard Cite

Induction and Activation of Adaptive Immune Populations During Acute and Chronic Phases of a Murine Model of Experimental Colitis

Cited by 92 publications

References 29 publications

Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis

Multifunctional Activity of a Small Tellurium Redox Immunomodulator Compound, AS101, on Dextran Sodium Sulfate-induced Murine Colitis

A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression

Local immune compartments are related to the severity of dextran sodium sulphate induced colitis

Contact Info

Product

Resources

About