Local immune compartments are related to the severity of dextran sodium sulphate induced colitis

Wang, Luman; Jiang, Xuechao; Liu, Xiaoming; Qian, Tingting; Chu, Yiwei

doi:10.5582/bst.2014.01088

Cited by 2 publications

(1 citation statement)

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“…Chemical disruption of the gut mucosa with DSS induces inflammation in the absence of B or T cells, although T cells have been shown to play a role in the recovery phase following induction of mild colitis 26 , 27 . Administration of 3% DSS results in severe colitis with acute inflammation of the colon involving infiltration of granulocytes and macrophages in the mucosa 28 .…”

Section: Discussionmentioning

confidence: 99%

Ceramide Synthase 6 Deficiency Enhances Inflammation in the DSS model of Colitis

Helke

Angel

Lü

et al. 2018

Sci Rep

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Colitis, an inflammatory disease of the digestive tract, is increasing in incidence and prevalence. Intestinal inflammation can occur as a consequence of dysfunctions in sphingolipid metabolism. In this study we used ceramide synthase 6 (CerS6) deficient mice, which have a reduced ability to generate long chain C16-ceramide, to investigate the role of this enzyme in dextran sodium salt (DSS)-induced colitis. While CerS6-deficient mice are protected from T cell mediated colitis, in the T cell independent DSS model lack of CerS6 resulted in a more rapid onset of disease symptoms. CerS6-deficient mice maintained low levels of C16-ceramide after DSS treatment, but the inflammatory lipid sphingosine-1-phosphate was significantly increased in colon tissue. In the absence of CerS6, DSS induced more severe pathology in the colon including enhanced neutrophil infiltration. In vivo analysis of myeloperoxidase activity, an enzyme released from neutrophils, was approximately 2.5-fold higher in CerS6-deficient mice compared to wild type. Differences in intestinal permeability did not account for the increase in neutrophils. Our study suggests that lack of CerS6 expression differentially impacts the development of colitis, depending on the model used.

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Section: Discussionmentioning

confidence: 99%