Inducible Metabolic Adaptation Promotes Mesenchymal Stem Cell Therapy for Ischemia

Zhu, Hongming; Sun, Aijun; Zou, Yunzeng; Ge, Junbo

doi:10.1161/atvbaha.114.303194

Cited by 51 publications

(42 citation statements)

References 35 publications

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“…Four treatments were done: normoxia A decrease in the concentration of environmental oxygen regulates the expression of many genes [56] and the stress caused by hypoxia provokes an acute increase in the rate of glucose transport. In mammalian cells hypoxia stimulates Glut1 expression and glucose transport [8,57,58] and the Glut1 promoter has hypoxia response elements recognized by HIF-1 (hypoxia inducible factor 1) [59]. The GLUT1 regulation by hypoxia has not been reported in crustaceans but, it is well known that in L. vannamei hypoxia induces a shift from aerobic to anaerobic metabolism with lactate accumulation [3][4][5].…”

Section: Hypoxia Regulates Lvglut1 Expressionmentioning

confidence: 99%

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

et al. 2014

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During hypoxia the shrimp Litopenaeus vannamei accelerates anaerobic glycolysis to obtain energy; therefore, a correct supply of glucose to the cells is needed. Facilitated glucose transport across the cells is mediated by a group of membrane embedded integral proteins called GLUT; being GLUT1 the most ubiquitous form. In this work, we report the first cDNA nucleotide and deduced amino acid sequences of a glucose transporter 1 from L. vannamei. A 1619 bp sequence was obtained by RT-PCR and RACE approaches. The 5´ UTR is 161 bp and the poly A tail is exactly after the stop codon in the mRNA. The ORF is 1485 bp and codes for 485 amino acids. The deduced protein sequence has high identity to GLUT1 proteins from several species and contains all the main features of glucose transporter proteins, including twelve transmembrane domains, the conserved motives and amino acids involved in transport activity, ligands binding and membrane anchor. Therefore, we decided to name this sequence, glucose transporter 1 of L. vannamei (LvGLUT1). A partial gene sequence of 8.87 Kbp was also obtained; it contains the complete coding sequence divided in 10 exons. LvGlut1 expression was detected in hemocytes, hepatopancreas, intestine gills, muscle and pleopods. The higher relative expression was found in gills and the lower in hemocytes. This indicates that LvGlut1 is ubiquitously expressed but its levels are tissue-specific and upon short-term hypoxia, the GLUT1 transcripts increase 3.7-fold in hepatopancreas and gills. To our knowledge, this is the first evidence of expression of GLUT1 in crustaceans.

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Section: Hypoxia Regulates Lvglut1 Expressionmentioning

confidence: 99%

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

et al. 2014

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“…Although the methods described here do not describe the complete impact of NIr light treatment on cellular metabolism in the vestibular sensory epithelium, further application of this strategy can be modified to include other age-associated markers of mitochondrial function 28,29 and/ or cellular metabolic insults such as hypoxia 30 . Ultimately, the ability to describe the vestibular cellular metabolic profile of an individual mouse will allow the investigation of the correlations between balance performance and subcellular processes during ageing, and the impact of therapeutics including NIr treatment.…”

Section: Representative Resultsmentioning

confidence: 99%

Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Zhang

Tung

Mathews

et al. 2015

JoVE

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Strategies for attenuating decline in balance function with increasing age are predominantly focused on physical therapies including balance tasks and exercise. However, these approaches do not address the underlying causes of balance decline. Using mice, the impact of near infrared light (NIr) on the metabolism of cells in the vestibular sensory epithelium was assessed. Data collected shows that this simple and safe intervention may protect these vulnerable cells from the deleterious effects of natural aging. mRNA was extracted from the isolated peripheral vestibular sensory epithelium (crista ampullaris and utricular macula) and subsequently transcribed into a cDNA library. This library was then probed for the expression of ubiquitous antioxidant (SOD-1). Antioxidant gene expression was then used to quantify cellular metabolism. Using transcranial delivery of NIr in young (4 weeks) and older (8 -9 months) mice, and a brief treatment regime (90 sec/day for 5 days), this work suggests NIr alone may be sufficient to improve mitochondrial function in the vestibular sensory epithelium. Since there are currently no available, affordable, non-invasive methods of therapy to improve vestibular hair cell function, the application of external NIr radiation provides a potential strategy to counteract the impact of aging on cellular metabolism inthe vestibular sensory epithelium. Video LinkThe video component of this article can be found at

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“…To improve their retention and survival, researchers performed a series of studies and found that aldehyde dehydrogenase-2 (ALDH2) plays an important role in microenvironment homeostasis after ischemia. 56,57 One million green fluorescent protein (GFP) mesenchymal stem cells were transplanted into mice where limb ischemia model had been achieved by the ligation of femoral artery, and ALDH2-deficient tissue markedly reduced stem cell retention, blood perfusion recovery, and limb salvage effects after vascular injury. Moreover, overexpression of ALDH2 greatly enhanced mesenchymal stem cell survival via increased capillary density, oxidative stress regulation, and energy supply after ischemia, indicating that ALDH2 is a key enzyme for stem cell therapy.…”

Section: Mesenchymal Stem Cells and Hematopoietic Stem Cellsmentioning

confidence: 99%

Vascular Regeneration by Stem/Progenitor Cells

Xie

Fan

2016

ATVB

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Inducible Metabolic Adaptation Promotes Mesenchymal Stem Cell Therapy for Ischemia

Abstract: HP-induced glycogen storage improves MSC survival and therapy in ischemic tissues. Thus, inducible metabolic adaptation in stem cells may be considered as a novel strategy for potentiating stem cell therapy for ischemia.

Cited by 51 publications

References 35 publications

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

The glucose transporter 1 -GLUT1- from the white shrimp Litopenaeus vannamei is up-regulated during hypoxia

Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Vascular Regeneration by Stem/Progenitor Cells

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